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Imaging in Virus Research
- 1st Edition, Volume 116 - July 25, 2023
- Editors: Stefan Finke, Dmitry Ushakov
- Language: English
- Hardback ISBN:9 7 8 - 0 - 4 4 3 - 1 5 8 1 4 - 8
- eBook ISBN:9 7 8 - 0 - 4 4 3 - 1 5 8 1 5 - 5
Imaging in Virus Research, Volume 116 in the Advances in Virus Research series highlights new advances in the field, with this new volume presenting interesting chapters writ… Read more
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Request a sales quoteImaging in Virus Research, Volume 116 in the Advances in Virus Research series highlights new advances in the field, with this new volume presenting interesting chapters written by an international board of authors. Topics of interest in this release include Recent developments of advanced fluorescence microscopy methods for biological applications, Fluorescence microscopy to image virus entry: Probing different angles, RSV and cytoplasmic inclusion bodies, Advanced Imaging of HIV-1 fusion and virus-host lipid interactions, Spatiotemporal orchestration of virus morphogenesis, Imaging applied to study interactions between poxviruses and their host cells, Viral capsid structures, Tissue Clearing and 3D-Imaging of Virus Infections, and much more.
- Provides the authority and expertise of leading contributors from an international board of authors
- Presents the latest release in the Advances in Virus Research serial
- Updated release includes the latest information on Imaging in Virus Research
Researchers, students, and academics in the field of virus research
- Cover
- Title page
- Table of Contents
- Series Page
- Copyright
- Contributors
- Chapter One: Spatial resolution of virus replication: RSV and cytoplasmic inclusion bodies
- Abstract
- 1: Introduction
- 2: Characterizing RSV IBs
- 3: Conclusions
- References
- Further reading
- Chapter Two: From the beginnings to multidimensional light and electron microscopy of virus morphogenesis
- Abstract
- 1: Introduction: Imaging viral morphogenesis in three dimensions (3D)
- 2: Imaging viral morphogenesis in 3D using EM
- 3: Correlative light- and electron microscopic approaches for imaging viral morphogenesis
- 4: Novel methods and future directions
- References
- Chapter Three: Tissue optical clearing and 3D imaging of virus infections
- Abstract
- 1: Introduction—3D fluorescence imaging
- 2: 3D imaging of optically cleared tissues in virus research
- 3: 3D tissue imaging of virus vectors for neuronal tracing, gene- and oncolytic therapy
- 4: 3D tissue imaging in virus and pathogenicity research
- 5: 3D image analysis
- 6: Conclusions
- 7: Outlook
- Acknowledgment
- References
- Chapter Four: Advanced fluorescence microscopy in respiratory virus cell biology
- Abstract
- 1: Introduction
- 2: Fluorescence microscopy methods to study respiratory virus replication
- 3: Respiratory syncytial virus
- 4: Influenza A virus
- 5: Severe acute respiratory syndrome coronavirus 2
- 6: Perspective
- Acknowledgments
- References
- Chapter Five: Shedding light on reovirus assembly—Multimodal imaging of viral factories
- Abstract
- 1: Introduction
- 2: Reoviridae and avian reovirus
- 3: Fluorescent imaging of viral factories
- 4: Holotomographic phase microscopy
- 5: Confocal Raman microscopy
- 6: Electron microscopy
- 7: Concluding remarks
- Acknowledgments
- References
- No. of pages: 258
- Language: English
- Edition: 1
- Volume: 116
- Published: July 25, 2023
- Imprint: Academic Press
- Hardback ISBN: 9780443158148
- eBook ISBN: 9780443158155
SF
Stefan Finke
Prof. Dr. Stefan Finke, is the Head of the Institute and Laboratory Head of the Institute of Molecular Virology and Cell Biology (IMVZ) of Friedrich-Loeffler-Institut, the Federal Research Institute for Animal Health of Germany.
Affiliations and expertise
Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, GermanyDU
Dmitry Ushakov
Dmitry Ushakov is a Head of Imaging and Bioinformatics laboratory at Friedrich Loeffler Institute, Riems Island. His current research is centered on understanding the fundamental principles governing virus-host interactions and associated immune cell responses. He obtained his PhD in Biochemistry at the University of North Texas Health Science Center at Fort Worth.
In his early research carrier at Imperial College London, Hanover Medical School and University of Leeds, he carried out development of advanced fluorescence imaging methods with modalities ranging from high-resolution imaging, to ratiometric imaging, such as fluorescence lifetime imaging microscopy, to super-resolution fluorescence microscopy. He applied them to studies across scales investigating biological motility, organelle traffic and signalling in immune, epithelial and muscle cells. He later became interested in cell behavior in complex 3D environment of biological tissues in homeostasis and in response to stress or infection. Working at King’s College London and the Crick Institute, he developed a high-throughput immune cell screening platform, which combined confocal microscopy with automated multi-parameter quantitative 3D image analysis.
Recently his laboratory established novel approaches for tissue optical clearing, volumetric imaging and 3D image analysis enabling quantification of large tissue volumes and whole organs in different animals. This technology has been applied to study infection by a number of neurotropic and respiratory viruses, such as Rabies virus and SARS-CoV-2.
Affiliations and expertise
Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, GermanyRead Imaging in Virus Research on ScienceDirect