High-Performance Liquid Chromatography

Contents

Conspectus

Preface

Reversed-Phase Chromatography of Proteins and Nucleic Acids: Practical Considerations

I. Introduction

II. Equipment Requirements

III. Column Selection

IV. Mobile-Phase Effects

V. Other Practical Considerations

VI. Hydrophobic Interaction Chromatography

VII. RP-HPLC of Oligonucleotides

VIII. Summary

References

Mixed-Interaction Stationary Phases in HPLC

I. Introduction

II. Concept of Mixed-Interaction Chromatography

III. Biopolymer Separations

IV. Low-Pressure Chromatography

V. High-Performance Liquid Chromatography

VI. High-Performance Mixed-Mode Chromatography

VII. Conclusions

References

Liquid Chromatography/Electrochemistry

I. Introduction

II. Basic Principles of LCEC

III. Practical Operational Considerations in LCEC

IV. Multipotential Detection in LCEC

V. Selected Applications of Liquid

Chromatography/Electrochemistry

References

High-Performance Liquid Chromatography Separations of Large Molecules: A General Model

I. Introduction

II. Small-Molecule Retention

III. Small-Molecule Band Broadening

IV. Large-Molecule HPLC: Retention Relationships

V. Large-Molecule HPLC: Band Broadening

VI. Separation and Resolution

VII. Non-Well-Behaved Samples

VIII. The Design of Columns for Macromolecular HPLC

IX. Other Models of Macromolecular HPLC

X. Conclusions

Appendix I. Use of "Normal versus Superficial" Mobile-Phase Velocity: Effect on C Term of Knox Equation

Appendix II. Examples of Calculations by the Model of Table VIII

Glossary of Symbols

References

Index