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High-Performance Liquid Chromatography
Advances and Perspectives
- 1st Edition - January 5, 1987
- Editor: Csaba Horváth
- Language: English
- Hardback ISBN:9 7 8 - 0 - 1 2 - 3 1 2 2 0 4 - 9
- Paperback ISBN:9 7 8 - 1 - 4 8 3 2 - 4 0 1 3 - 8
- eBook ISBN:9 7 8 - 1 - 4 8 3 2 - 6 1 8 8 - 1
High-Performance Liquid Chromatography: Advances and Perspectives, Volume 4 is an authoritative publication that deals with the fundamentals, instrumentation, and applications of… Read more
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Request a sales quoteHigh-Performance Liquid Chromatography: Advances and Perspectives, Volume 4 is an authoritative publication that deals with the fundamentals, instrumentation, and applications of high-performance liquid chromatography. The volume contains articles on practical aspects of reversed-phase chromatography in the study of biopolymer separations; characterization of stationary phases and the development of various packing materials; electrochemical detection; and the fundamentals of chromatographic behavior of large molecules. Chromatographers, chemists, and researchers in the field of chemical analysis will find this book an interesting read.
Contents
Conspectus
Preface
Reversed-Phase Chromatography of Proteins and Nucleic Acids: Practical Considerations
I. Introduction
II. Equipment Requirements
III. Column Selection
IV. Mobile-Phase Effects
V. Other Practical Considerations
VI. Hydrophobic Interaction Chromatography
VII. RP-HPLC of Oligonucleotides
VIII. Summary
References
Mixed-Interaction Stationary Phases in HPLC
I. Introduction
II. Concept of Mixed-Interaction Chromatography
III. Biopolymer Separations
IV. Low-Pressure Chromatography
V. High-Performance Liquid Chromatography
VI. High-Performance Mixed-Mode Chromatography
VII. Conclusions
References
Liquid Chromatography/Electrochemistry
I. Introduction
II. Basic Principles of LCEC
III. Practical Operational Considerations in LCEC
IV. Multipotential Detection in LCEC
V. Selected Applications of Liquid
Chromatography/Electrochemistry
References
High-Performance Liquid Chromatography Separations of Large Molecules: A General Model
I. Introduction
II. Small-Molecule Retention
III. Small-Molecule Band Broadening
IV. Large-Molecule HPLC: Retention Relationships
V. Large-Molecule HPLC: Band Broadening
VI. Separation and Resolution
VII. Non-Well-Behaved Samples
VIII. The Design of Columns for Macromolecular HPLC
IX. Other Models of Macromolecular HPLC
X. Conclusions
Appendix I. Use of "Normal versus Superficial" Mobile-Phase Velocity: Effect on C Term of Knox Equation
Appendix II. Examples of Calculations by the Model of Table VIII
Glossary of Symbols
References
Index
- No. of pages: 332
- Language: English
- Edition: 1
- Published: January 5, 1987
- Imprint: Academic Press
- Hardback ISBN: 9780123122049
- Paperback ISBN: 9781483240138
- eBook ISBN: 9781483261881
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