High-Performance Liquid Chromatography

Contents

List of Contributors

Conspectus

Preface

Evolution of Liquid Chromatography: A Historical Overview

I. The Field as We See It Today

II. The Beginnings

III. The Dormant Period

IV. The Rebirth of Chromatography

V. Variants of Chromatography

VI. High-Performance Liquid Chromatography

VII. Postword

References

Practical Operation of Bonded-Phase Columns in High-Performance Liquid Chromatography

I. Introduction

II. Types of Bonded-Phase Chromatography

III. Characteristics of Silica Gel

IV. Preparation of Bonded Phases

V. Phase Chain Length and Its Effect

VI. Columns for Bonded-Phase Chromatography

VII. Columns for Normal Bonded-Phase Chromatography

VIII. Modes of Reverse-Phase Chromatography

IX. Columns for Reverse-Phase Chromatography

X. Ion-Pair Chromatography

XI. Other Experimental Variables in Bonded-Phase

Chromatography

XII. Conclusions

References

Secondary Chemical Equilibria in High-Performance Liquid Chromatography

I. Introduction

II. Fundamental Concepts

III. Chemical Principles of Ionic Equilibria

IV. Chromatography

V. Conclusion

References

Gradient Elution

I. Introduction

II. A Simplified Picture of Separation in Gradient Elution

III. Experimental Considerations

IV. Designing and Improving a Gradient Separation

V. Theoretical Relationships

VI. Conclusion

List of Symbols

References

Index