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Helicase Enzymes Part B

  • 1st Edition - August 12, 2022
  • Latest edition
  • Editor: Michael Trakselis
  • Language: English

Methods in Enzymology serial highlights new advances in the field with this new volume presenting interesting chapters. Each chapter is written by an international board of author… Read more

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Description

Methods in Enzymology serial highlights new advances in the field with this new volume presenting interesting chapters. Each chapter is written by an international board of authors.

Key features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in Methods in Enzymology serials
  • Updated release includes the latest information on Helicase Enzymes

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Table of contents

1. Crosslinking immunoprecipitation of Upf1 in yeast
Kristian Baker

2. Biochemical Analysis of the SARS-CoV-2 Nsp13 Helicase Implicated in COVID-19
Robert Brosh

3. Bulk phase biochemistry of the Pif1 helicase
Matthew Bochman

4. Translational control by helicases during cellular stress
Tim Bolger

5. Measuring DDX3-dependent protein synthesis in vitro and in cells
Stephen Floor

6. Purification of Mtr4 and Mtr4-mediated complexes
Sean Johnson

7. Plan of attack for studying DEAD-box proteins
Katrin Karbstein

8. Reconstitution, purification, and activity characterization for RNA-guided helicase-nuclease machines in Type I CRISPR-Cas systems
Ailong Ke

9. Helicase mediated vectorial folding of G-quadruplex
Sua Myong

10. Detecting G4 quadruplex unwinding
Katrin Paeschke

11. Monitoring RNA and ATP-dependent conformational changes in RIG-I-like receptors using fluorescence microscopy
Anna Marie Pyle

12. ATP utilization measurements in RNA unwinding and RNA chaperone activities by helicase proteins
Rick Russell

13. Hydrogen-Deuterium Exchange Mass Spectrometry of RNA Helicases
Sheena D'Arcy

14. Biochemical characterization of human MTR4-RNA exosome and adaptor complexes
Christopher D. Lima

15. Study of RNA helicases with magnetic tweezers
Herve Le Hir

16. Single-molecule FRET to analyze the dynamics of RNA helicases
Dagmar Klostermeier

17. Transcriptional regulation by a RecQ helicase
Sudha Sharma

18. In vitro characterization of the DEXH helicase Dhr1 from yeast
Arlen Johnson

Product details

  • Edition: 1
  • Latest edition
  • Published: August 16, 2022
  • Language: English

About the editor

MT

Michael Trakselis

Michael grew up in a western suburb of Chicago called Aurora, IL, or better known as Wayne’s World. He got his BS in Psychology and Chemistry at the University of Illinois, Urbana-Champaign. After which, he employed as a technician working on analytical method development at Abbott Laboratories. From 1998-2002 he was a PhD student at Penn State University working in Stephen Benkovic lab on the Bacteriophage T4 replisome assembly and dynamics. In 2003, he started a postdoctoral fellowship in Ron Laskey’s laboratory at the University of Cambridge/Hutchison MRC in the UK. In 2006, he started his independent career at the University of Pittsburgh Chemistry department. Starting 2014, he moved his laboratory to Baylor University, where he currently lives with his family. There, the Trakselis laboratory is currently studying aspects of DNA replication and repair spanning from the very in vitro biochemical to the very in vivo cell and human biology.
Affiliations and expertise
Associate Professor and Director of Graduate Affairs, Baylor University, TX, USA

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