Handbook of Immunoassay Technologies

Approaches, Performances, and Applications

2nd Edition - February 4, 2025
Imprint: Academic Press
Editors: Sandeep K. Vashist, John H.T. Luong
Language: English
Paperback ISBN:
9 7 8 - 0 - 3 2 3 - 9 5 5 0 9 - 6
eBook ISBN:
9 7 8 - 0 - 3 2 3 - 9 5 5 1 0 - 2

Handbook of Immunoassay Technologies: Approaches, Performances, and Applications, Second Edition unravels the role of immunoassays in the biochemical sciences. During the last f… Read more

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Handbook of Immunoassay Technologies: Approaches, Performances, and Applications, Second Edition unravels the role of immunoassays in the biochemical sciences. During the last four decades, a wide range of immunoassays has been developed, ranging from the conventional enzyme-linked immunosorbent assays to the smartphone-based point-of-care formats. The book discusses how advances in rapid biochemical procedures, novel biosensing schemes, fully integrated lab-on-a-chip platforms, prolonged biomolecular storage strategies, device miniaturization and interfacing, and emerging smart system technologies that have paved the way for next-generation immunoassays.

Revised and updated, the second edition of Handbook of Immunoassay Technologies: Approaches, Performances, and Applications covers all the relevant, timely, and important developments in the field. This edition offers new content on topics such as antibody production for immunodiagnostics, multiplex immunoassays, chemiluminescent immunoassays, immunoassays for newborn screening, and immunoassays of viruses like SARS-CoV-2, HIV, Ebola, and Hepatitis C. The addition of these new topics as well as up-to-date content make the second edition a valuable and comprehensive resource on immunoassays.

Title of Book
Cover image
Title page
Table of Contents
Copyright
List of contributors
Preface
Objectives
Scope
Target audience
Book organization
Chapter 1. Immunoassays: an overview
Abstract
1 Overview of immunoassays
2 Antibody structure
3 Need for immunoassays
4 Immunoassay formats
5 Conclusions and future trends
References
Chapter 2. Antibody immobilization and surface functionalization chemistries for immunodiagnostics
Abstract
1 Introduction
2 Surface functionalization chemistries
3 Antibody immobilization chemistries
4 Surface characterization
5 Conclusions, challenges, and future trends
References
Chapter 3. Monoclonal antibody generation by phage display: history, state-of-the-art, and future
Abstract
1 Introduction
2 Phage display selection
3 Antibody libraries
4 In vitro selection of antibodies for specific applications
5 Site-specific conjugation and modification of antibody functionality
6 Conclusion and outlook
References
Chapter 4. Bioanalytical requirements and regulatory guidelines for immunoassays
Abstract
1 Introduction
2 Bioanalytical requirements for an immunoassay
3 Critiques and outlook
4 Conclusions
References
Chapter 5. Enzyme-linked immunoassays
Abstract
1 Introduction
2 Conventional enzyme-linked immunoassays
3 Emerging enzyme-linked immunoassays
4 Portable analyzer–based immunoassays
5 Critiques and outlook
6 Conclusions
References
Chapter 6. Chemiluminescent immunoassays (CLIA)
Abstract
1 Introduction
2 Chemiluminescent immunoassay mechanisms
3 Commercial chemiluminescence immunoassay analyzers
4 Commercial and potential aspects of CLIA
5 Conclusions
References
Chapter 7. Lateral flow immunoassays
Abstract
1 Introduction
2 Advances in lateral flow immunoassays
3 Challenges and future directions
4 Conclusions
Acknowledgments
References
Chapter 8. Paper-based immunoassays
Abstract
1 Introduction
2 Paper-based immunoassays: strategies and detection principles
3 Development of the paper-based immunoassays devices
4 Conclusions
References
Chapter 9. Acoustic wave–based immunoassays
Abstract
1 Introduction
2 Clinical diagnostics
3 Detection of microbial pathogens and toxins
4 Detection of parasites
5 Detection of viruses
6 Quartz crystal microbalance and surface acoustic wave-based electronic noses
7 Quartz crystal microbalance and surface acoustic wave immunoassays in environmental monitoring and allergens detection
8 Integrated acoustic wave immunosensors for point of care
9 Commercial acoustic wave immunosensors
10 Market potential and conclusions
Acknowledgments
References
Chapter 10. Interferometry-based immunoassays
Abstract
1 Introduction—general context
2 Principles of operation
3 Sensor surface functionalization
4 Conclusions and future perspectives
References
Chapter 11. Nanomaterial- and micromaterial-based immunoassays
Abstract
1 Introduction
2 Micromaterial-based immunoassay
3 Colorimetric immunoassay
4 Electrochemical immunoassay
5 Fluorescent immunoassay
6 Conclusion
References
Chapter 12. Microcantilever-based sensors
Abstract
1 Introduction
2 Microcantilevers and their modes of operation
3 Detection methods
4 Bending behavior of microcantilevers
5 Fabrication of microcantilevers
6 Microcantilever-based sensors
7 Electronic nose
8 Nanocantilevers
9 Commercial availability
10 Conclusions and future trends
References
Chapter 13. Quartz crystal microbalance–based sensors
Abstract
1 Introduction
2 Detection of biomolecules
3 Detection of bacteria
4 Detection of volatile organic compounds
5 Detection of chemical analytes
6 Detection of gaseous analytes
7 Special analytical applications
8 Other analytical applications
9 Conclusions and future trends
References
Further reading
Chapter 14. Electrochemical immunosensors fundamentals and applications in clinical diagnostics
Abstract
1 Introduction
2 Electrochemical techniques in immunosensing
3 Advanced approaches in electrochemical immunosensors
4 Biorecognition elements in immunosensors
5 Electrochemical immunosensors
6 Conclusions and outlook
Acknowledgments
References
Chapter 15. Lab-on-a-chip immunoassays
Abstract
1 Introduction
2 Lab-on-a-chip immunoassays
3 Critiques and outlook
4 Conclusions
References
Chapter 16. Smartphone-based immunoassays
Abstract
1 Introduction
2 Signal detection in smartphone-based immunoassays
3 Applications of smartphone-based immunoassays
4 Conclusions, challenges, and future trends
References
Chapter 17. Multiplex immunoassays
Abstract
1 Introduction
2 Multiplex immunoassay formats
3 Multiplex immunoassays
4 Critiques and outlook
5 Conclusions
References
Chapter 18. Immunoassays for newborn screening
Abstract
1 Introduction
2 TSH immunoassay
3 Immunoassay for 17-OHP
4 Immunoassay for PAP
5 Conclusion
References
Chapter 19. Biotin interference in immunoassays and strategies to tackle it
Abstract
1 Introduction
2 Levels of biotin interferences
3 Mitigation of biotin interference
4 Modification of the assay procedure
5 Immunoassay format redesign
6 Detection of biotin
7 Conclusions and future possibilities
References
Chapter 20. In vitro diagnostic immunoassays for SARS-CoV-2
Summary
1 Introduction
2 Immunoassays for SARS-CoV-2
3 Targets of detecting SARS-CoV-2
4 Detection formats
5 Challenges and future directions
6 Conclusions
References
Chapter 21. Immunoassays of human immunodeficiency virus (HIV)
Abstract
1 Introduction
2 Detection targets of HIV
3 Nucleic acid amplification technology (NAT)
4 Immunoassays
5 Confirmatory HIV test
6 Assays using nanomaterials
7 Trends and concluding remarks
References
Further reading
Chapter 22. Immunoassays of hepatitis C virus (HCV)
Abstract
1 Introduction
2 Detection of HCV core antigen (HCVcAg)
3 Detection of anti-HCV antibodies
4 Conclusion
References
Chapter 23. Immunoassays for Ebola virus disease
Abstract
1 Introduction
2 Characteristics of Ebolavirus
3 Laboratory diagnosis methods
4 Concluding remarks
References
Chapter 24. Immunoassays of botulism neurotoxins (BoNTs) of Clostridia
Abstract
1 Introduction
2 Key features of botulinum neurotoxins
3 Immunoassays
4 Assays for endopeptidase (EP)
5 Other biosensing platforms
6 Trends and future possibilities
7 Conclusions
References
Chapter 25. Immunoassays of biological warfare agents
Abstract
1 Introduction
2 General aspects of the immunoassays
3 Immunoassays of biological agents
4 Trends and future possibilities
5 Conclusions
References
Chapter 26. Lateral flow immunoassays with improved detection sensitivity
Abstract
1 Introduction
2 Principles of lateral flow immunoassays
3 Detection schemes
4 Conclusions
References
Chapter 27. Immunoassays: future prospects and possibilities
Abstract
1 Immunoassays: trends and prospects
2 Evolving healthcare
3 Challenges
4 Future directions
References
Index

Sandeep K. Vashist

Dr. Sandeep K. Vashist is the Senior Global IVD Product Director at Fapon Biotech Inc., Germany. He held senior R&D and management roles in large IVD giants, such as Bristol-Myers Squibb, Revvity (Immunodiagnostic Systems), and Trivitron Healthcare (Labsystems Diagnostics). Besides, he has been the Chief of the Research Advisory Board at Sensing Self Pte Ltd., Singapore, an Advisory Board Member at Calciscon AG, Switzerland, and a Board Member at Antrum Biotech, South Africa. Sandeep invented and pioneered many CE-certified and/or FDA-approved IVD kits, devices, and technologies. He has 12 patents, over 300 publications, 3 books, and over 9000 international citations. He is the Editor and Reviewer of several journals, funding agencies, and international organizations. Sandeep is an IVD veteran with over two decades of rich global IVD experience.

Affiliations and expertise

Senior Global IVD Product Director at Fapon Biotech Inc., Germany

John H.T. Luong

Prof John H.T. Luong was the Walton Fellow associated with the School of Chemistry of University College Cork, Ireland. He was the Professor and Division Head of Biomedical Engineering at Nanyang Technological University, Singapore. He was also a distinguished Visiting Professor at the University of Zhengzhou (China), a Visiting Professor at Nanocore, National University of Singapore, Bar-Ilan University (Ramat Gan-Israel), Institut d'Electronique, de Microélectronique et de Nanotechnologie, Université de Lille (France), and Delhi Technological University, India. He has received several awards for his excellent contribution to research, innovation, and technology transfer. He is on the Editorial Board of several Journals, the Chief Editor of MDPI Journal of Functional Biomaterials-Antimicrobials, and the Associate Editor of MDPI Macromolecule. He has 10 patents and 350 publications with over 31,000 citations with an h-index of 89. He is the expert/reviewer/advisor/consultant for many international funding agencies and enterprises.

Affiliations and expertise

Walton Fellow and Adjunct Professor, Department of Chemistry, University College Cork, Ireland

Life Sciences

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Social Sciences & Humanities

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