
Handbook of Current and Novel Protocols for the Treatment of Infertility
- 1st Edition - September 6, 2023
- Imprint: Academic Press
- Editors: Michael H. Dahan, Human M. Fatemi, Nikolaos P. Polyzos, Juan A. Garcia-Velasco
- Language: English
- Paperback ISBN:9 7 8 - 0 - 3 2 3 - 8 5 6 8 7 - 4
- eBook ISBN:9 7 8 - 0 - 3 2 3 - 9 0 1 0 6 - 2
The field of Assisted Reproductive Technology (ART) is rapidly evolving and stimulation protocols, fertility strategies and aspects of infertility treatments are constantly being… Read more

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Request a sales quoteThe field of Assisted Reproductive Technology (ART) is rapidly evolving and stimulation protocols, fertility strategies and aspects of infertility treatments are constantly being updated as advances and new discoveries are made.
Handbook of Current and Novel Protocols for the Treatment of Infertility is a valuable resource of well-organized, comprehensive scientific data with practical guides and step-by-step protocols for infertility management. Written by world-wide experts, this book discusses different practice patterns and approaches used internationally and presents innovative topics including preimplantation genetic testing, time lapse imaging and the role of artificial intelligence in ART.
This book provides up-to-date, evidence-based guidance on daily practice and is an important resource for infertility providers, including trainees or experienced clinicians, embryologist, and is geared for both specialist in reproductive medicine and gynecologists interested in increasing their knowledge in the field of reproductive endocrinology and intertility.
Handbook of Current and Novel Protocols for the Treatment of Infertility is a valuable resource of well-organized, comprehensive scientific data with practical guides and step-by-step protocols for infertility management. Written by world-wide experts, this book discusses different practice patterns and approaches used internationally and presents innovative topics including preimplantation genetic testing, time lapse imaging and the role of artificial intelligence in ART.
This book provides up-to-date, evidence-based guidance on daily practice and is an important resource for infertility providers, including trainees or experienced clinicians, embryologist, and is geared for both specialist in reproductive medicine and gynecologists interested in increasing their knowledge in the field of reproductive endocrinology and intertility.
- Presents protocols for infertility management and new developments in practical techniques and understanding, including discussions on in vitro maturation, in vitro fertilization and ovarian stimulation
- Discusses innovative topics such as the role of artificial intelligence in infertility management, protocols using progesterone to prevent ovulation, dual-stim protocols, random start protocols, complications in IVF, and management of these complications
- Chapter written by well-known experts on infertility management from different parts of the world, thus providing a worldwide perspective
Physicians practicing fertility care, IVF specialist, embryologist, infertility specialists, reproductive endocrinologists, specialist in reproductive medicine, lab technicians, gynecologists and obstetricians
- Cover image
- Title page
- Table of Contents
- Copyright
- Dedication
- Dedication
- Dedication
- Dedication
- List of contributors
- Preface
- Chapter 1. The evaluation of the female infertility patient
- History assessment
- Physical exam
- Pelvic ultrasound
- Evaluation of ovarian reserve
- Ovulation assessment
- Tubal patency evaluation
- Other investigations
- Conclusion
- Chapter 2. The fertility evaluation of the male partner
- Overview
- Clinical history
- Physical examination
- Semen analysis
- Hormonal evaluation
- Genetic studies
- Conditions with special considerations
- Chapter 3. Sperm DNA fragmentation and male infertility: a comprehensive review for the clinicians
- Sperm genetics
- How do sperm genetics become dysfunctional?
- Single- or double-strand breaks: does it really matter?
- Which test to assess sperm chromatin integrity and SDF?
- Etiologies and risk factors for SDF
- The impact of SDF on male reproductive outcomes
- How to manage patients with high SDF?
- Clinical practice guidelines and recommendations on SDF testing
- Limitations of sperm SDF testing in clinical practice?
- Updates in the recent research on SDF (2019–21)
- Clinical scenarios on SDF management
- Conclusion
- Chapter 4. The evaluation and management of recurrent pregnancy loss
- Introduction
- Incidence and prevalence
- Etiology and risk factors
- Evaluation of RPL
- Prevention
- Treatment
- Future directions
- Conclusion
- Chapter 5. The role of surgery in infertility, an evidence-based approach
- Introduction
- Chapter 6. Ovarian stimulation and intrauterine insemination
- Introduction
- Definition and indications
- Ovarian stimulation protocols
- Final remarks
- Chapter 7. Infertility care of the PCOS patient
- Introduction
- Preconception risk factors
- Weight loss and obesity
- Pharmacologic treatment strategies
- Letrozole
- Selective estrogen receptor modulators
- Gonadotropins
- Considerations for administration of ovulation induction agents
- In vitro fertilization/Intracytoplasmic sperm injection
- In vitro maturation
- Inositol
- Laparoscopic ovarian drilling
- Acupuncture, herbal remedies, and traditional Chinese medicine
- Conclusion
- Chapter 8. The GnRH antagonist protocol
- Introduction
- GnRH antagonist protocols
- Cetrorelix versus ganirelix
- GnRH antagonist fixed protocol
- GnRH antagonist flexible protocol
- Fixed versus flexible GnRH antagonist protocol
- Pretreatment with oral contraceptive pill in a GnRH antagonist cycle
- Elevated progesterone at initiation of stimulation in a GnRH antagonist cycle
- FSH dose in GnRH antagonist protocols
- Initiation of FSH dose in GnRH antagonist protocols
- Administration of antagonist in the early follicular phase
- Delayed start GnRH antagonist protocol
- Luteal GnRH antagonist administration
- Criteria for triggering final oocyte maturation
- GnRH antagonists and OHSS
- The modified natural cycle
- Use of GnRH antagonists in ovarian stimulation for intrauterine insemination
- Luteal phase support in antagonist cycles
- Alternative to GnRH antagonists LH suppression regimens
- Chapter 9. The long gonadotropin releasing hormone agonist IVF protocol
- Introduction
- A brief history of IVF attempts
- Gonadotropin-releasing hormone agonists
- Conclusions
- Chapter 10. Double stimulation in the same ovarian cycle (DuoStim) and luteal phase start IVF protocols
- Introduction
- Theories of folliculogenesis
- Definition of poor prognosis patients
- An unconventional ovarian stimulation protocol: luteal phase stimulation
- DuoStim for poor prognosis patients: the evidence up to date
- Framework of a DuoStim protocol
- Clinical indications for DuoStim
- DuoStim: is the second stimulation in the same ovarian cycle conducted in the luteal phase pf the cell cycle?
- Conclusions
- Chapter 11. The mild stimulation in vitro fertilization protocol
- Mild ovarian stimulation for IVF
- Chapter 12. The use of progestins to prevent the LH surge in IVF cycles
- Alternative protocols for ovarian stimulation
- Mechanism of pituitary suppression by progestins
- Progestins
- PPOS protocols: outcomes and schemes of treatment
- Advantages and limits
- Chapter 13. Triggering final oocyte maturation. The role of human chorionic gonadotropin, gonadotropin-releasing hormone agonist, and dual trigger
- Introduction
- Human chorionic gonadotropin, a surrogate to the naturally occurring LH surge
- hCG versus GnRHa trigger
- Triggering final follicular maturation in patients not at risk to develop severe OHSS
- GnRHa and hCG in patients at risk to develop severe OHSS
- Conclusions
- Chapter 14. Pathogenesis and management in OHSS
- Introduction
- Pathogenesis
- OHSS management
- Mild OHSS
- Moderate OHSS
- Severe and critical OHSS
- Critical OHSS
- Resolution and prognosis
- Chapter 15. Complications of in vitro fertilization (excluding ovarian hyperstimulation syndrome) and their management
- Introduction
- Complications of ovarian stimulation: specific aspects and management
- Complications of the egg retrieval procedure
- Intermediate/long-term complications of IVF
- Conclusion
- Chapter 16. In vitro maturation of immature cumulus–oocyte complexes collected from antral follicles
- What is oocyte in vitro maturation?
- Clinical outcomes of standard IVM as an artificial reproductive technology
- Selection of appropriate patients suitable for IVM treatment
- Standard IVM: clinical and laboratory protocols
- Chapter 17. Stimulated in vitro oocyte maturation
- Introduction
- Oocyte physiology
- Potential indications for IVM
- IVM in the era of antagonist protocol with agonist trigger
- IVM instead of natural cycle IVF
- IVM instead of FSH/IUI
- IVM and fertility preservation for cancer
- IVM and resistant ovary syndrome
- Protocols
- Patient preparation
- Fertilization
- Aspiration technique and culture
- Embryo transfer
- Safety concerns
- Conclusion
- Chapter 18. Fertility preservation
- Introduction
- Indications
- Cryopreservation techniques
- Clinical approach
- Conclusion
- Chapter 19. Preimplantation genetic testing
- The origins of PGT
- Different kinds of PGT and their indications
- Biopsy techniques
- PGT-M: molecular analysis
- Comprehensive chromosome testing techniques
- Clinical evidence supporting PGT-A
- Chromosomal mosaicism, intermediate copy numbers, and segmental aneuploidies
- Alternative sources of DNA
- Chapter 20. Nutritional supplements and other adjuvants in fertility care
- Introduction
- Growth hormone
- Dehydroepiandrosterone
- Chapter 21. Maximizing fertility outcomes in poor ovarian response patients
- Introduction
- Treatment
- Adjuvant treatments
- Challenges in determining evidence-based interventions
- Conclusion
- Chapter 22. Endometrial receptivity
- Introduction
- Relevance and concept of endometrial receptivity
- Mechanisms of endometrial receptivity
- Transcriptomics in bulk tissue
- Transcriptomics at the single-cell level
- Future directions
- Conclusions
- Chapter 23. Impact of the microbiome in fertility
- Microbiome
- Microbiome and infertility
- Microbiome and gynecological diseases
- Microbiome, obstetrics, and the offspring
- Seminal microbiome
- Treatment possibilities
- Conclusion
- Chapter 24. How to manage recurrent implantation failure, what do we know?
- Definition and incidence
- Treatment options in RIF
- Prognosis
- Conclusion
- Chapter 25. How to set up an andrology laboratory for a fertility center?
- Introduction
- The design
- Equipment
- Quality assurance
- Accreditation
- Air quality
- Hygienic maintenance before use
- Quality control check for automated and manual sperm counting methods
- Cryopreservation
- Cryostorage standards and requirements
- Cryopreservation of infectious samples
- Sperm functioning tests
- Chapter 26. How to set up an in vitro fertilization laboratory
- The planning team
- Regulatory requirements and professional guidelines
- Cleanroom design and space requirements
- Chapter 27. Introduction to the in vitro fertilization laboratory: management of sperm, oocytes, and embryos
- A holistic perspective of the IVF laboratory
- Equipment
- Quality management
- Gamete retrieval and preparation—oocytes
- Gamete retrieval and preparation—sperm
- Standard/conventional insemination
- Intracytoplasmic sperm injection
- Embryo culture
- Embryo grading and selection
- Embryo transfer
- Summary
- Chapter 28. Introduction to the IVM laboratory
- Oocyte maturation process
- Oocyte identification
- IVM of immature oocytes, culture medium for IVM and supplements
- Culture time for assessing in vitro maturation of immature oocytes
- Insemination of mature oocytes produced from IVM cycles
- Optimal ICSI timing
- Culture of IVM embryos and embryo transfer
- Cryopreservation of oocytes and embryos obtained from IVM cycles
- Most challenging aspects to introduce IVM into the routine laboratory
- Chapter 29. Time-lapse imaging
- Introduction
- Background of time-lapse systems
- Implementation of artificial intelligence in assisted reproduction laboratories
- Conclusions
- Chapter 30. Troubleshooting the in vitro fertilization laboratory
- Introduction
- Process mapping and process control
- Using root cause analysis
- Is the origin of a problem always found?
- Proactive troubleshooting
- The value of quality
- Take home messages
- Index
- Edition: 1
- Published: September 6, 2023
- Imprint: Academic Press
- No. of pages: 464
- Language: English
- Paperback ISBN: 9780323856874
- eBook ISBN: 9780323901062
MD
Michael H. Dahan
Michael H. Dahan is American board certified in Obstetrics and Gynecology and Reproductive Endocrinology as well as in Infertility, and serves as professor in the department of Obstetrics and Gynecology at McGill University, Canada. With over 140 peer reviewed articles published, he is recognized as one of the leaders in the field of fertility. He has pioneered many of the current novel treatments in In Vitro Fertilization and is a member of the Poseidon group as expert in poor ovarian response. Dr Dahan is the Editor-in-Chief of the Journal of Clinical and Experimental Obstetrics & Gynecology (CEOG) and former professor at Stanford University in California and Washington University in St Louis.
Affiliations and expertise
Professor, Department of Obstetrics and Gynecology, McGill University, CanadaHF
Human M. Fatemi
Human M. Fatemi has been a professor at the Brussels University and is the director of middle east operations for IVI, a company exclusively devoted to human Assisted Reproduction Technology. He has authored over 80 scientific papers in infertility and has been an invited speaker at 150 conferences worldwide. He completed medical school as Magna Cum Laude in 1999 at the Vrije Universiteit Brussel, in Belgium. In 2008, he completed his Ph.D. on the endocrinology of the luteal phase in stimulated and substituted cycles. Also in 2008, he was recognized as a subspecialist in Reproductive Medicine and Surgery by ESHRE (European society of Human Reproduction) and EBCOG (European Board and College of Obstetrics and Gynecology).
Affiliations and expertise
Medical Director, IVI Middle East, United Arab EmiratesNP
Nikolaos P. Polyzos
Nikolaos Polyzos is the Clinical and Scientific Director of the Department of Reproductive Medicine of Dexeus University Hospital in Barcelona, Spain, and a Professor of Reproductive Endocrinology in the Department of Clinical Medicine in the University of Aarhus, Denmark. He has served as a Medical Director in the Centre for Reproductive Medicine in Brussels until 2017 and as a Professor in Brussels until 2019. Professor Polyzos is the principal investigator in several large pioneering multinational trials and a key opinion leader in the field of Reproductive Medicine for the treatment of poor ovarian responders in IVF programs, prediction of ovarian response and premature ovarian ageing, with more than 150 invited lectures in international congresses. He is a Section Editor in reproductive Biomedecine Online, member of the ESHRE guideline development group for ovarian stimulation and has served as an Editorial Consultant for the PIER (Physician’s Information and Education Resource) of the American College of Physicians (ACP).
Affiliations and expertise
Director, Department of Reproductive Medicine, Quirón Dexeus University Hospital, SpainJG
Juan A. Garcia-Velasco
Juan García-Velasco is Director of IVI Madrid. He is also Professor of Obstetrics and Gynaecology at Rey Juan Carlos University, Madrid, Spain, where he is Director of their Master’s Degree Programme in Human Reproduction. He graduated from University Medical School, Madrid, in 1990 and received his Obstetrics and Gynecology certification from La Paz Hospital, Madrid, in 1995. He completed his PhD in Medicine from Autonoma University, Madrid, in 1995 and from 1997 to 1998 he studied at Yale University, New Haven, CT, under a Reproductive Endocrinology and Infertility Fellowship. His main research interests have been in IVF and endometriosis. He is the Principal Investigator of projects funded by the Ministry of Education and Ministry of Health, Spain, and has received awards from the Spanish Fertility Society, Spanish Society of Obstetrics and Gynecology, and the European Society of Human Reproduction and Embryology. He has published over 200 peer‐reviewed articles as well as 22 book chapters on human reproduction, endometriosis and hypo‐ and hyper‐ovarian stimulation response and is the author/editor of several books.
Affiliations and expertise
Professor of Obstetrics and Gynecology, Rey Juan Carlos University, Madrid, Spain
Director IVI RMA - Madrid, SpainRead Handbook of Current and Novel Protocols for the Treatment of Infertility on ScienceDirect