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Guide to Research Techniques in Neuroscience

2nd Edition - February 27, 2015

Authors: Matt Carter, Jennifer C. Shieh

Paperback ISBN:

9 7 8 - 0 - 1 2 - 8 0 0 5 1 1 - 8

eBook ISBN:

9 7 8 - 0 - 1 2 - 8 0 0 5 9 7 - 2

Neuroscience is, by definition, a multidisciplinary field: some scientists study genes and proteins at the molecular level while others study neural circuitry using… Read more

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Neuroscience is, by definition, a multidisciplinary field: some scientists study genes and proteins at the molecular level while others study neural circuitry using electrophysiology and high-resolution optics. A single topic can be studied using techniques from genetics, imaging, biochemistry, or electrophysiology. Therefore, it can be daunting for young scientists or anyone new to neuroscience to learn how to read the primary literature and develop their own experiments.

This volume addresses that gap, gathering multidisciplinary knowledge and providing tools for understanding the neuroscience techniques that are essential to the field, and allowing the reader to design experiments in a variety of neuroscience disciplines.

Foreword to the Second Edition
Foreword to the First Edition
Preface to the Second Edition
Preface to the First Edition
Introduction
Chapter 1. Whole-Brain Imaging
- Structural Brain Imaging Techniques
- Functional Brain Imaging Techniques
- Functional Imaging Experimental Design and Analysis
- Conclusion
Chapter 2. Animal Behavior
- Considerations for Choosing and Performing a Behavioral Assay
- Rodent Behavioral Paradigms
- Drosophila Behavioral Paradigms
- Caenorhabditis elegans Behavioral Paradigms
- Nonhuman Primate Behavioral Paradigms
- Conclusion
Chapter 3. Stereotaxic Surgeries and In Vivo Techniques
- Stereotaxic Surgeries
- Implants for Long-Term Access to the Brain
- Manipulating Neural Activity In vivo
- Conclusion
Chapter 4. Electrophysiology
- A Brief Review of the Electrical Properties of Neurons
- The Electrophysiology Rig
- Categories of Electrophysiology Recordings
- Electrophysiology Tissue Preparations
- Manipulating Neural Activity during Electrophysiology Experiments
- Conclusion
Chapter 5. Microscopy
- Essential Principles of Microscopy
- Light Microscopy
- Fluorescence Microscopy
- Electron Microscopy
- Preparing and Interpreting Microscopy Data
- Conclusion
Chapter 6. Visualizing Neural Structure
- Tissue Preparation
- Visualizing Morphology
- Visualizing Gene and Protein Expression
- Visualizing Circuitry
- Conclusion
Chapter 7. Visualizing Neural Function
- Static Markers of Activity
- Visualizing Neural Activity
- Visualizing Protein Function
- Conclusion
Chapter 8. Manipulating Neural Activity
- Experimental Logic of Manipulating Neural Activity
- Physical Manipulation
- Electrical Manipulation
- Pharmacological Manipulation
- Genetic Manipulation
- Pharmacogenetic Manipulation
- Optogenetic Manipulation
- Neuromodulation Techniques Used in Humans
- Conclusion
Chapter 9. Identifying Genes and Proteins of Interest
- A Brief Review of Genes and Proteins
- Genetic Screens
- In silico Screens
- Molecular Screens
- Conclusion
Chapter 10. Molecular Cloning and Recombinant DNA Technology
- Isolating DNA Fragments
- Cloning DNA
- Identifying DNA
- Conclusion
Chapter 11. Gene Delivery Strategies
- Physical Gene Delivery
- Chemical Gene Delivery
- Viral Gene Delivery
- Conclusion
Chapter 12. Making and Using Transgenic Organisms
- Transgenes
- The Transgenic Construct
- Binary Transgenic Systems
- Making Transgenic Organisms
- Conclusion
Chapter 13. Manipulating Endogenous Genes
- Classical Gene Targeting Methods
- Nuclease-Based Gene Targeting Methods
- Disrupting Gene Products
- Conclusion
Chapter 14. Cell Culture Techniques
- Cell Culture Equipment and Reagents
- Immortalized Cell Lines
- Primary Cell and Tissue Culture
- Stem Cell Cultures
- Manipulating Cells in Culture
- Conclusion
Chapter 15. Biochemical Assays and Intracellular Signaling
- Introduction to Signal Transduction and Intracellular Signaling
- Fundamental Tools Used to Study Proteins
- Investigating Protein Expression
- Investigating Protein–Protein Interactions
- Investigating Posttranslational Modifications
- Investigating Protein–DNA Interactions
- Conclusion
Glossary
Index