Guide to Protein Purification
- 1st Edition, Volume 182 - February 28, 1990
- Imprint: Academic Press
- Editors: Murray P. Deutscher, John N. Abelson, Melvin I. Simon
- Language: English
- eBook ISBN:9 7 8 - 0 - 0 8 - 0 8 8 2 9 9 - 4
Guide to Protein Purification, designed to serve the needs of the student, experienced researcher and newcomer to the field, is a comprehensive manual that provides all the up-to-… Read more
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Request a sales quoteGuide to Protein Purification, designed to serve the needs of the student, experienced researcher and newcomer to the field, is a comprehensive manual that provides all the up-to-date procedures necessary for purifying, characterizing, and handling proteins and enzymes in one source.
@introbul:Key Features
@bul:* Detailed procedures newly written for this volume
* Extensive practical information
* Rationale and strategies for protein and enzyme purification
* Personal perspectives on enzyme purification by eminent researchers
@introbul:Among the Topics Covered
@bul:* General methods for handling proteins and enzymes
* Extraction, subcellular fractionation, and solubilization procedures
* Comprehensive purification techniques
* Specialized purification procedures
* Protein characterization
* Immunological procedures
* Computer analysis of protein structure
@bul:* Detailed procedures newly written for this volume
* Extensive practical information
* Rationale and strategies for protein and enzyme purification
* Personal perspectives on enzyme purification by eminent researchers
@introbul:Among the Topics Covered
@bul:* General methods for handling proteins and enzymes
* Extraction, subcellular fractionation, and solubilization procedures
* Comprehensive purification techniques
* Specialized purification procedures
* Protein characterization
* Immunological procedures
* Computer analysis of protein structure
Protein biochemists, enzymologists, analytical biochemists, graduate students, and postdoctorates in these disciplines, cell and molecular biologists, biotechnologists, and industrial researchers working with protein products.
(Section Headings): Developing Purification Procedures. General Methods for Handling Proteins and Enzymes. Optimization of Starting Materials. Preparation of Extracts and Subcellular Fractionation. Solubilization Procedures. Purification Procedures: Bulk Methods. Purification Procedures: Chromatographic Methods. Purification Procedures: Electrophoretic Methods. Specialized Purification Procedures. Characterization of Purified Proteins. Immunological Procedures. Additional Techniques. Concluding Remarks. Appendix: Personal Perspectives on Enzyme Purification. Author Index. Subject Index.
- Edition: 1
- Volume: 182
- Published: February 28, 1990
- Imprint: Academic Press
- No. of pages: 894
- Language: English
- eBook ISBN: 9780080882994
MD
Murray P. Deutscher
Affiliations and expertise
University of Connecticut Health Center, Farmington, Connecticut, U.S.A.JA
John N. Abelson
Affiliations and expertise
California Institute of Technology, Division of Biology, Pasadena, U.S.A.MS
Melvin I. Simon
Affiliations and expertise
The Salk Institute, La Jolla, CA, USARead Guide to Protein Purification on ScienceDirect