
Guide to Plant Single-Cell Technology
Functional Genomics and Crop Improvement
- 1st Edition - November 25, 2024
- Imprint: Academic Press
- Editor: Jen-Tsung Chen
- Language: English
- Paperback ISBN:9 7 8 - 0 - 4 4 3 - 2 3 7 3 6 - 2
- eBook ISBN:9 7 8 - 0 - 4 4 3 - 2 3 7 3 7 - 9
Guide to Plant Single-Cell Technology: Functional Genomics and Crop Improvement summarizes the current status of single-cell technology in plants involving food and energy cr… Read more

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Request a sales quoteGuide to Plant Single-Cell Technology: Functional Genomics and Crop Improvement summarizes the current status of single-cell technology in plants involving food and energy crops. Presenting methods and applications of emerging high-throughput technologies performed using the single-cell platform it includes an emphasis on single-cell RNA sequencing and eventually towards single-cell omics, which are highly complementary and effective for profiling the plant cell subject to either environmental factors or pathogenic threats. These technologies can advance the exploration of plant physiology as well as precision crop breeding for future anti-stress and high-yield plants and achieve sustainable agriculture.
The book covers crop improvement and breeding strategies involving single-cell technology to produce future stress-tolerant and high-yield plants, which have better performances on growth, and development to achieve enhanced production of foods and biomass.
Guide to Plant Single-Cell Technology: Functional Genomics and Crop Improvement will be a valuable reference resource for academics and researchers in plant and crop sciences.
- Focuses on plant molecular profiling using single-cell technology and the integration with functional genomics
- Discusses the current methods and challenges of single-cell RNA sequencing in plants
- Summarizes the emerging findings of plant single-cell technology
- Presents advanced high-throughput technologies for plant omics
- Title of Book
- Cover image
- Title page
- Table of Contents
- Copyright
- List of contributors
- About the editor
- Preface
- Chapter 1. An overview of single-cell high-throughput technology in plants
- Abstract
- Chapter outline
- 1.1 The need for and emergence of single-cell high-throughput technologies
- 1.2 Current methods and advanced technologies in single-cell (epi)genomics
- 1.3 To infinity and beyond: future challenges in the single-cell world
- Acknowledgments
- References
- Chapter 2. Understanding single-cell multi-omics for high resolution cellular interactome mapping
- Abstract
- Chapter outline
- 2.1 Introduction
- 2.2 Advancement in single-cell epigenomics
- 2.3 Advancement in single-cell transcriptomics
- 2.4 Advancement in spatial transcriptomics
- 2.5 Advancement in single-cell proteomics
- 2.6 Challenges in plant single-cell omics
- Author contributions
- Conflicts of Interest
- Acknowledgments
- References
- Chapter 3. Single cell technology for studying plant growth and development
- Abstract
- Chapter outline
- 3.1 Introduction
- 3.2 Diversified approaches related to single-cell omics in plants
- 3.3 Implementations of single-cell technologies for crop improvement
- 3.4 Conclusions and future way-out
- References
- Chapter 4. Single-cell technology for studying plant–microbe interactions
- Abstract
- Chapter outline
- 4.1 Introduction
- 4.2 Single-cell technologies for studying plant–microbe interactions
- 4.3 Applications of single-cell technologies to plant–microbe interactions
- 4.4 Advantages and challenges of single-cell technologies
- 4.5 Conclusions and future directions
- Acknowledgments
- References
- Chapter 5. Single-cell technology for disease-resistant plants
- Abstract
- Chapter outline
- 5.1 Introduction
- 5.2 Plant defense system mechanism
- 5.3 Plant disease resistance complexities
- 5.4 Different single-cell technologies
- 5.5 Role of single-cell technology for disease-resistant plants
- 5.6 The significance of tissue heterogeneity for disease-resistant plants
- 5.7 Conclusion
- References
- Chapter 6. Single-cell technology for plant systems biology
- Abstract
- Chapter outline
- 6.1 Introduction
- 6.2 Technological innovations benefit plant single-cell omics
- 6.3 Biological considerations in plant single-cell omics
- 6.4 In silico approach to analyze single-cell omics datasets
- 6.5 Conclusions and perspectives
- References
- Chapter 7. Single-cell technology for plant biotechnology
- Abstract
- Chapter outline
- 7.1 Introduction
- 7.2 Plant biotechnological approaches used in different eras
- 7.3 Single-cell isolation techniques
- 7.4 Exploring plant single cell and single cell type
- 7.5 Single-cell analysis in biotechnology, systems biology, and biocatalysis
- 7.6 Methodologies in single-plant cell proteomics
- 7.7 Applications of single-plant cell proteomics
- 7.8 Challenges and future directions
- 7.9 Methodologies in single-plant cell metabolomics
- 7.10 Applications of single-plant cell metabolomics
- 7.11 Challenges and future directions
- 7.12 Applications in biotechnology
- 7.13 Challenges and future perspectives
- 7.14 Conclusion
- References
- Further reading
- Chapter 8. Single-cell technology for crop breeding
- Abstract
- Chapter outline
- 8.1 Introduction
- 8.2 Current global status of the use of single-cell technology in crop breeding
- 8.3 Developing stress-tolerant plant variety via single-cell technology
- 8.4 Components of single-cell technology
- 8.5 Application of single-cell technology in crop breeding
- 8.6 Challenges and prospects
- 8.7 Conclusion
- References
- Chapter 9. Single-cell technology for plant metabolomics
- Abstract
- Chapter outline
- 9.1 Introduction
- 9.2 Single-cell omics technologies
- 9.3 Single-cell omics applications in plant research
- 9.4 Data analysis and interpretation
- 9.5 Integration of single-cell metabolomics with other omics approaches
- 9.6 Challenges and limitations
- 9.7 Conclusion and future directions
- References
- Chapter 10. Single-cell technology for plant natural product research
- Abstract
- Chapter outline
- 10.1 Introduction
- 10.2 Single-cell technology approaches
- 10.3 Plant cell culture technology for pharmaceutically active natural product synthesis and supply
- 10.4 Plant cell culture: a revolutionary approach to natural product formation
- 10.5 Single-cell technology unveils the wonders of plant natural products
- 10.6 Production of natural products
- 10.7 Problems and challenges
- 10.8 Perspectives and future directions
- 10.9 Conclusion
- References
- Chapter 11. Single-cell omics to dissect molecular features in plant root development
- Abstract
- Chapter outline
- 11.1 Introduction
- 11.2 Discovering new cell identities
- 11.3 Root vasculature at cellular resolution
- 11.4 Regeneration of the root apical meristem
- 11.5 Postembryonic formation of lateral roots
- 11.6 Organogenesis of nitrogen fixing nodules
- 11.7 Future perspectives
- Funding
- AI disclosure
- References
- Chapter 12. Single-cell technology for improvement in significant crops
- Abstract
- Chapter outline
- Overview
- 12.1 Introduction
- 12.2 Single-cell transcriptomics for crop research
- 12.3 Single-cell approaches for stress response in crops
- 12.4 Multiomics strategies for trait mapping and gene discovery
- 12.5 Challenges and opportunities in multiomics research
- References
- Chapter 13. Annotation of single-cell clusters using marker genes within and across species
- Abstract
- Chapter outline
- 13.1 Introduction
- 13.2 Overview of published single-cell data in plants
- 13.3 Data analytic pipelines for single-cell analysis
- 13.4 Cross-species comparison and annotation of single-cell data
- 13.5 Challenges and limitations
- 13.6 Future directions
- References
- Chapter 14. Machine learning prediction of novel gene functions with single-cell sequencing data
- Abstract
- Chapter Outline
- 14.1 Introduction
- 14.2 History and significance of machine learning in plant functional genomics
- 14.3 Methodology
- 14.4 Results
- 14.5 Conclusion
- References
- Index
- Edition: 1
- Published: November 25, 2024
- Imprint: Academic Press
- No. of pages: 400
- Language: English
- Paperback ISBN: 9780443237362
- eBook ISBN: 9780443237379
JC
Jen-Tsung Chen
Dr. Jen-Tsung Chen is a professor of cell biology at the National University of Kaohsiung in Taiwan. He also teaches genomics, proteomics, plant physiology, and plant biotechnology. Dr. Chen’s research interests include bioactive compounds, chromatography techniques, plant molecular biology, plant biotechnology, bioinformatics, and systems pharmacology. He is an active editor of academic books and journals to advance the exploration of multidisciplinary knowledge involving plant physiology, plant biotechnology, nanotechnology, ethnopharmacology, systems biology, and drug discovery. He serves as an editorial board member and a guest editor in several reputed journals. Dr. Chen published books in collaboration with international publishers on diverse topics such as drug discovery, herbal medicine, medicinal biotechnology, nanotechnology, bioengineering, plant functional genomics, plant speed breeding, CRISPR-based plant genome editing, and artificial intelligence. In 2023, Dr. Chen has been included in the World's Top 2% Scientists by Stanford University.