Growth, Nutrition, and Metabolism of Cells In Culture V2

List of Contributors

Preface

Contents of Volume I


1. General Introduction

Text

References


2. Use of Perfusion Systems for Growth of Cell and Tissue Cultures

I. Introduction

II. Stationary Culture Perfusion Systems

III. Rolling Bottle Perfusion Culture Systems

IV. Suspension Culture Perfusion Systems

V. Performance Aspects and Applications of Perfusion Systems

VI. Conclusions

References


3. Cultivation of Muscle Tissue

I. Introduction

II. Culture Methods

III. Experimental Analysis of Myogenesis In Vitro

References


4. Cultivation of Nerve Tissue

I. Historical Perspective

II. Methods

III. Nutritional and Metabolic Requirements for Maintenance or Growth, and Metabolic Characteristics of Nervous Tissue in Culture

IV. Experimental Neurophathology in Culture

V. Conclusion

References


5. Cultivation of Hematopoietic Cells

I. Introduction

II. Early Studies on Culture of Lymphoid Cells

III. Sources of Established Lines

IV. Factors Affecting Isolation

V. Cultural Characteristics

VI. Nutritional and Metabolic Requirements

VII. Products of Hemic Cell Lines

VIII. Other Studies

IX. Comment

References


6. Hormone Synthesis and Function In Vitro

I. Introduction

II. Energy Metabolism by Hormone-Secreting Endocrine Tissue

III. Culture Methods

IV. Hormone-Secreting Tissues and Their Actions In Vitro

V. Physiological Considerations in Hormone Action

References


7. Cultivation of the Mammalian Embryo

I. Introduction

II. Techniques

III. Physical Environment

IV. Nutritional Substrates

V. Typical Media

VI. Assessing Results

VII. Conclusions

References


8. Cultivation of Cells from Poikilothermic Vertebrates

I. Introduction

II. Nutritional Requirements for Cell Culture

III. Temperature Requirements for Cell Culture

IV. Biochemical Studies of Poikilothermic Vertebrate Cells

V. Summary

References


9. Cultivation of Arthropod Cells

I. Introduction

II. Nutritional Requirements of Arthropod Cells

III. Problems in Media Design

IV. Culture Techniques

V. Applications of Arthropod Tissue Culture

References


10. Cultivation of Plant Cells

I. Introduction

II. Methods

III. Maintenance and Preservation of Cultures

IV. Cultivation of Single Cells

V. Mutants

VI. Protoplasts

VII. Haploid Cell Cultures

VIII. Growth of Plant Cells in Culture

IX. Stability of Cultures

X. Characterization of Plant Cell Lines

XI. Nutrition

XII. Morphogenesis in Plant Cell Cultures

XIII. Metabolism

XIV. Regulation of Metabolism

References

Author Index

Subject Index