Growth, Nutrition, and Metabolism of Cells In Culture V2
- 1st Edition - January 1, 1972
- Imprint: Academic Press
- Editor: George Rothblat
- Language: English
- Hardback ISBN:9 7 8 - 0 - 1 2 - 5 9 8 3 0 2 - 0
- Paperback ISBN:9 7 8 - 0 - 1 2 - 4 1 4 6 3 4 - 1
- eBook ISBN:9 7 8 - 0 - 3 2 3 - 1 4 9 5 3 - 2
Growth, Nutrition, and Metabolism of Cells in Culture, Volume 2, summarizes the state of knowledge of the growth, nutrition, and metabolism of various types of cell cultures. The… Read more
Purchase options
Institutional subscription on ScienceDirect
Request a sales quoteGrowth, Nutrition, and Metabolism of Cells in Culture, Volume 2, summarizes the state of knowledge of the growth, nutrition, and metabolism of various types of cell cultures. The chapters are both detailed and comprehensive enough for the specialist and broad enough to provide a general background for the nonspecialist. The present volume deals with specialized mammalian, plant, and invertebrate cell systems and techniques. The book begins by tracing the history of the development of tissue culture. This is followed by separate chapters on the use of perfusion systems in cell and tissue culture; and the cultivation of muscle tissue, nerve tissue, and hematopoietic cells. Subsequent chapters discuss the use of cell culture to study mechanisms of hormone action; the cultivation of mammalian embryos; cultivation of cells from poikilothermie vertebrates; and the cultivation of arthropod cells and plant cells. This book will be valuable resource for investigators who routinely use cell culture techniques, as well as students and individuals in associated areas of cell and molecular biology.
List of Contributors
Preface
Contents of Volume I
1. General Introduction
Text
References
2. Use of Perfusion Systems for Growth of Cell and Tissue Cultures
I. Introduction
II. Stationary Culture Perfusion Systems
III. Rolling Bottle Perfusion Culture Systems
IV. Suspension Culture Perfusion Systems
V. Performance Aspects and Applications of Perfusion Systems
VI. Conclusions
References
3. Cultivation of Muscle Tissue
I. Introduction
II. Culture Methods
III. Experimental Analysis of Myogenesis In Vitro
References
4. Cultivation of Nerve Tissue
I. Historical Perspective
II. Methods
III. Nutritional and Metabolic Requirements for Maintenance or Growth, and Metabolic Characteristics of Nervous Tissue in Culture
IV. Experimental Neurophathology in Culture
V. Conclusion
References
5. Cultivation of Hematopoietic Cells
I. Introduction
II. Early Studies on Culture of Lymphoid Cells
III. Sources of Established Lines
IV. Factors Affecting Isolation
V. Cultural Characteristics
VI. Nutritional and Metabolic Requirements
VII. Products of Hemic Cell Lines
VIII. Other Studies
IX. Comment
References
6. Hormone Synthesis and Function In Vitro
I. Introduction
II. Energy Metabolism by Hormone-Secreting Endocrine Tissue
III. Culture Methods
IV. Hormone-Secreting Tissues and Their Actions In Vitro
V. Physiological Considerations in Hormone Action
References
7. Cultivation of the Mammalian Embryo
I. Introduction
II. Techniques
III. Physical Environment
IV. Nutritional Substrates
V. Typical Media
VI. Assessing Results
VII. Conclusions
References
8. Cultivation of Cells from Poikilothermic Vertebrates
I. Introduction
II. Nutritional Requirements for Cell Culture
III. Temperature Requirements for Cell Culture
IV. Biochemical Studies of Poikilothermic Vertebrate Cells
V. Summary
References
9. Cultivation of Arthropod Cells
I. Introduction
II. Nutritional Requirements of Arthropod Cells
III. Problems in Media Design
IV. Culture Techniques
V. Applications of Arthropod Tissue Culture
References
10. Cultivation of Plant Cells
I. Introduction
II. Methods
III. Maintenance and Preservation of Cultures
IV. Cultivation of Single Cells
V. Mutants
VI. Protoplasts
VII. Haploid Cell Cultures
VIII. Growth of Plant Cells in Culture
IX. Stability of Cultures
X. Characterization of Plant Cell Lines
XI. Nutrition
XII. Morphogenesis in Plant Cell Cultures
XIII. Metabolism
XIV. Regulation of Metabolism
References
Author Index
Subject Index
- Edition: 1
- Published: January 1, 1972
- No. of pages (eBook): 459
- Imprint: Academic Press
- Language: English
- Hardback ISBN: 9780125983020
- Paperback ISBN: 9780124146341
- eBook ISBN: 9780323149532
Read Growth, Nutrition, and Metabolism of Cells In Culture V2 on ScienceDirect