Growth, Nutrition, and Metabolism of Cells in Culture, Volume 2, summarizes the state of knowledge of the growth, nutrition, and metabolism of various types of cell cultures. The chapters are both detailed and comprehensive enough for the specialist and broad enough to provide a general background for the nonspecialist. The present volume deals with specialized mammalian, plant, and invertebrate cell systems and techniques. The book begins by tracing the history of the development of tissue culture. This is followed by separate chapters on the use of perfusion systems in cell and tissue culture; and the cultivation of muscle tissue, nerve tissue, and hematopoietic cells. Subsequent chapters discuss the use of cell culture to study mechanisms of hormone action; the cultivation of mammalian embryos; cultivation of cells from poikilothermie vertebrates; and the cultivation of arthropod cells and plant cells. This book will be valuable resource for investigators who routinely use cell culture techniques, as well as students and individuals in associated areas of cell and molecular biology.