Genome Engineering

1st Edition, Volume 52 - April 26, 2023
Imprint: Academic Press
Editors: Volker Gurtler, Michael Calcutt
Language: English
Hardback ISBN:
9 7 8 - 0 - 1 2 - 8 2 3 5 4 0 - 9
eBook ISBN:
9 7 8 - 0 - 1 2 - 8 2 3 5 4 2 - 3

Genome Engineering, Volume 52 in the Methods in Microbiology series, highlights new advances in the field with this new volume presenting interesting chapters written by an in… Read more

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Genome Engineering, Volume 52 in the Methods in Microbiology series, highlights new advances in the field with this new volume presenting interesting chapters written by an international board of authors. Topics covered include Whole genome transplantation in Mollicutes, Natural transformation as a tool in Acinetobacter baylyi: evolution by amplification of gene copy number, Natural transformation as a tool in Acinetobacter baylyi: streamlined engineering and mutational analysis, Methods to characterize cryptic biosynthetic gene clusters in Streptomyces, Genome engineering in bacteria: current and prospective applications, Tools for activation and identification of cryptic biosynthesis pathways in Streptomyces.

Cover image
Title page
Table of Contents
Series Page
Copyright
Contributors
Preface
References
Section I: Genome transformation
Chapter 1: Genome transplantation in Mollicutes
Abstract
1: The historical scientific context associated with genome transplantation
2: Mollicutes—The perfect model organisms for the establishment of GT
3: Baker's yeast—An engineering platform for microbial genomes
4: The GT protocol
5: Future perspectives
6: Ethical considerations
Acknowledgements
Appendix
References
Section II: Recombineering and engineering
Chapter 2: Genome engineering in bacteria: Current and prospective applications
Abstract
1: Non-CRISPR methods for bacterial genome editing
2: CRISPR-Cas systems and mechanisms
3: CRISPR as an adaptive immune mechanism in prokaryotes
4: Molecular mechanism
5: CRISPR-based genome editing methods
6: Current applications of CRISPR-Cas for genome engineering in bacteria
7: Alternative delivery methods for CRISPR-Cas: ribonucleoprotein complexes (RNPs) and lipid nanoparticles
8: Summary, conclusions and future directions
Acknowledgements
References
Chapter 3: Towards a circular bioeconomy: Engineering biology for effective assimilation of cellulosic biomass
Abstract
1: Introduction
2: Design
3: Build
4: Test
5: Learn and general considerations
6: Conclusions
References
Chapter 4: Recombineering
Abstract
1: Introduction
2: History and development of recombineering
3: Molecular tools for recombineering
4: Steps involved in a typical recombineering experiment
5: Uses of recombineering
6: Regulation and expression of recombineering gene
7: Recombineering in various systems
8: Future prospects of recombineering
References
Further reading
Section III: CRISPR
Chapter 5: Applications of CRISPR/Cas9 in the field of microbiology
Abstract
1: Overview of CRISPR/Cas9 biology
2: Applications of CRISPR/Cas9
3: Recent uses of CRISPR/Cas9-based technologies in microbiology
4: Techniques utilizing CRISPR/Cas9
5: Challenges in the field of CRISPR/Cas9 system
6: Conclusion
References
Chapter 6: Genome engineering in Aspergillus niger
Abstract
1: Introduction
2: Materials
3: Methods
4: Notes
References
Section IV: Transformation
Chapter 7: Natural transformation as a tool in Acinetobacter baylyi: Evolution by amplification of gene copy number
Abstract
1: Introduction
2: General considerations
3: Material and equipment
4: Experimental procedures
5: Summary and concluding remarks
Acknowledgements
References
Chapter 8: Natural transformation as a tool in Acinetobacter baylyi: Streamlined engineering and mutational analysis
Abstract
1: Introduction
2: General considerations
3: Material and equipment
4: Experimental procedures
5: Summary and concluding remarks
Acknowledgements
References

Volker Gurtler

Dr Gurtler is a Molecular biologist with extensive industry experience and expertise in DNA analysis for the identification and typing of organisms. He is known internationally as the author of the original methodology of ribotyping. He is Advisor to Geneious (Bioinformatics software company) and previous head of Molecular Biology at Austin Health Microbiology, currently Adjunct Professor at RMIT University, Editor of Journal of Microbiological Methods, Editor of Heliyon and Series Editor of Methods in Microbiology. Dr Gurtler is currently working in collaboration with Assoc. Prof Danilla Grando on ribosome analysis of E. coli isolates and association with virulence. He visits RMIT regularly to advise on experimental methods and help PhD students use Geneious Bioinformatic software.

Affiliations and expertise

Adjunct Professor, RMIT University, Melbourne, Australia

Michael Calcutt

Dr. Michael (Mick) Calcutt is a Professor and the Director of Graduate Studies for the Molecular Pathogenesis and Therapeutics Program at the Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri. He completed his BSc. in the University of Sheffield, UK, and his Ph.D in the University of Leicester, UK. Dr. Calcutt's research focuses on aspects of the molecular genetics and pathogenesis of Mycoplasmas, a diverse genus of cell wall-less bacteria that includes many important pathogens of food animals and humans. In the absence of a cell wall, the single limiting membrane represents the unique and critical interface between the pathogen and host. Analysis of the repertoire, function and potential antigenic variation of surface membrane proteins is one of the primary research interests in the laboratory, together with understanding chromosomal dynamics and the role of mobile genetic elements in such plasticity. Increasingly, whole genome sequencing and comparative genomic approaches are being exploited to systematically assess the deduced surface proteome, horizontal gene transfer and accessory gene pool that likely contribute to the pathobiology of these organisms.

Affiliations and expertise

Professor,Veterinary Pathobiology, Molecular Microbiology and Immunology, Missouri State University, USA

Life Sciences

Physical Sciences & Engineering

Social Sciences & Humanities

Health