Genetic Engineering and Genome Editing for Zinc Biofortification of Rice
- 1st Edition - June 23, 2023
- Imprint: Academic Press
- Editors: B.P. Mallikarjun Swamy, Anca Macovei, Kurniawan Rudi Trijatmiko
- Language: English
- Paperback ISBN:9 7 8 - 0 - 3 2 3 - 8 5 4 0 6 - 1
- eBook ISBN:9 7 8 - 0 - 3 2 3 - 8 5 4 0 7 - 8
Genetic Engineering and Genome Editing for Zinc Biofortification of Rice provides the first single-volume comprehensive resource on genetic engineering approaches, including novel… Read more
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Request a sales quoteGenetic Engineering and Genome Editing for Zinc Biofortification of Rice provides the first single-volume comprehensive resource on genetic engineering approaches, including novel genome editing techniques, to be carried out in rice, a staple crop for much of the world’s population. While zinc biofortification can be achieved through conventional breeding, genetic engineering, and agronomic practices, this book presents the latest developments, based on real-world experience and with targeted application. Dietary zinc deficiency can lead to negative health outcomes, including increased risk of stunting, respiratory diseases, diarrhea and mortality during childhood, and preterm births in pregnancy. Although providing access to diverse diet is an ideal solution to alleviate zinc deficiency, it may not be a viable solution in developing countries. Zinc biofortification, a process of increasing zinc concentration in the edible part of food crops, has been proposed as a sustainable approach that can reach people living in remote rural areas, complementing other interventions, such as supplementation and industrial fortification. Providing a complete view of the need for, and means of, zinc biofortification in rice, sections in this book discuss state-of-the-art scientific advances, and then goes further, placing them in their proper scientific, regulatory, and socioeconomic contexts. Thus, Genetic Engineering and Genome Editing for Zinc Biofortification of Rice is a valuable resource for graduate students, researchers, educators, librarians, and professionals in various kinds of institutions which focused on agriculture, environmental science, and plant science.
- Compiles the state-of-the-art information to allow fast-track understanding and application of zinc content improvement
- Discusses multiple strategic and methodology approaches
- Includes discussion of the socioeconomic implications of improved rice nutritional value
University graduate students (PhD), researchers and educators, Libraries and professionals of universities and various kinds of institutes focused on Agriculture, environmental science, plant science (crop improvement, plant breeding and genetics, plant molecular biology, plant protection, quality improvement)
- Cover image
- Title page
- Table of Contents
- Copyright
- List of contributors
- Foreword
- Preface
- Chapter 1. Molecular mechanisms leading to grain Zn accumulation in rice
- 1. Introduction
- 2. Conclusions
- Chapter 2. Molecular links between iron and zinc biofortification in rice
- 1. Introduction
- 2. Strategies of Fe and Zn uptake in rice
- 3. Long-distance Fe and Zn transport in rice
- 4. Fe and Zn biofortification in rice
- 5. Conclusions
- Chapter 3. Ionomics-based imaging, localization and quantification of zinc and other micronutrients in rice grains for biofortification research
- 1. Introduction
- 2. Role of ionomics in Zn biofortification in rice
- 3. Ionomics for high-throughput elemental analysis integrated with advanced technologies
- 4. Overview of elemental profiling in rice and other cereal crops
- 5. Destructive methods of element profiling
- 6. Nondestructive methods of element profiling
- 7. Ionomics in Zn biofortification—selected case studies
- 8. Future prospects for ionomics research in rice biofortification studies
- 9. Summary and conclusion
- Chapter 4. Recent advances in precise plant genome editing technology
- 1. Introduction
- 2. CRISPR/Cas system
- 3. CRISPR reagent delivery methods in plants
- 4. Dominance of NHEJ-mediated genome editing in somatic cells
- 5. Precise genome editing tools
- 6. Conclusion and future perspective
- 7. Conflict of interest
- Chapter 5. Practical protocol for design and construction of a transformation vector for prime editing in rice
- 1. Introduction
- 2. Design of transformation vector for prime editing in rice
- 3. Construction of transformation vector for prime editing in rice
- 4. Protocols
- Chapter 6. Stages of development of genetically modified (GM) plants
- 1. Introduction
- 2. Gene identification and crop transformation
- 3. Efficacy trials
- 4. Trait integration
- 5. Safety assessment of GM plants for food/feed safety
- 6. Seed production and product release
- 7. Stewardship
- 8. Conclusion
- Chapter 7. Nicotianamine enhances zinc transport to seeds for biofortification
- 1. Introduction
- 2. The role of nicotianamine (NA) in plant Zn and Fe homeostasis
- 3. Zn and NA transporters involved in Zn xylem and phloem loading
- 4. NAS genes as promising targets for Zn biofortification
- 5. Future prospects of Zn biofortification by NA
- 6. Conclusion
- Chapter 8. Zinc biofortification of rice by engineering metal transporter genes
- 1. Introduction
- 2. Zn transporters in rice plants
- 3. Zn biofortification through engineering expression of transporters
- 4. Concluding remarks and future directions
- Chapter 9. Reducing cadmium content in zinc biofortified rice through genetic manipulation
- 1. Introduction
- 2. Source of Cd contamination
- 3. Estimation of cadmium
- 4. Genetic variations of Cd accumulation in rice
- 5. Agronomic/crop management practices on Cd availability and accumulation
- 6. Association between Zn and Cd on crop growth and development
- 7. Current progress in low Cd high Zn biofortified rice
- 8. Development of high Zn biofortified rice with low Cd
- 9. Genes, transporters, and chelators associated with Cd and Zn
- 10. Transgenic and gene editing approach in low Cd high Zn biofortification
- 11. Future perspective
- Chapter 10. Improving bioavailability of zinc in rice grains by reducing antinutrients through genetic engineering
- 1. Phytic acid as an antinutrient
- 2. Managing Zn bioavailability through manipulating PA content
- 3. Discovery of genetic determinants
- 4. Genetic control of PA content
- 5. Future target of lpa rice
- Chapter 11. Leveraging intellectual property for the development of genome edited crops by public research institutes
- 1. Introduction
- 2. Definitions
- 3. Genome-editing projects for impact are long-term projects
- 4. Freedom to operate assessment on a country-specific basis
- 5. Material transfer agreements, donor agreements, and potential IP issues
- 6. FTO for R&D vs FTO for commercial use
- 7. Leveraging the public research institute intellectual property
- 8. Project-specific IPR landscape report and attorney legal opinion
- 9. Negotiating a licensing agreement with the owner of IP assets
- 10. Conclusion
- Chapter 12. Prospects and challenges associated with GM biofortified crops
- 1. Introduction
- 2. Malnutrition and micronutrient fortification
- 3. Transgenic crops biofortified with iron and zinc
- 4. Transgenic crops with vitamin A biofortification
- 5. Beyond biofortified crops
- 6. The regulatory approval process for biofortified crops developed using biotechnology
- 7. Consumer influence of the regulation of new breeding technologies
- 8. Conclusions
- 9. Future potential and prospects
- Chapter 13. Pathway from laboratory to market: regulatory approval process during the development of genetically modified crops—Philippine perspective
- 1. Introduction
- 2. The Philippine regulatory system for the entry and commercial use of GM crops
- 3. Implementation experience under JDC1, s2016
- 4. Important considerations in setting up the Philippine regulatory approval process for GM crops
- 5. Conclusion
- Chapter 14. Economic feasibility of genetically modified zinc-fortified rice
- 1. Introduction
- 2. Cost of developing and disseminating GM Zn-fortified rice
- 3. Benefits of GM zinc-fortified rice
- 4. Cost-effectiveness and benefit-cost of GM Zn-fortified rice in the Philippines
- 5. Conclusions
- Conflict of interest
- Declarations of interest
- Ethical approval
- Submission of revised manuscript
- Annex
- Index
- Edition: 1
- Published: June 23, 2023
- Imprint: Academic Press
- No. of pages: 240
- Language: English
- Paperback ISBN: 9780323854061
- eBook ISBN: 9780323854078
BS
B.P. Mallikarjun Swamy
PhD in Genetics, Osmania University, Hyderabad, India
MSc in Genetics and Plant Breeding, University of Agricultural Sciences, Dharwad, Karnataka, India;
BSc (Agriculture), University of Agricultural Sciences, Bangalore, Karnataka, India
Professional Experience:
Currently Senior Scientist I-Senior Breeder, IRRI, Los Banos, Laguna, Philippines
Scientist II- Rice Breeder, IRRI, Los Banos, Laguna, Philippines
Scientist I-Rice Breeder, at IRRI, Los Banos, Laguna, Philippines
Postdoctoral Fellow - at IRRI, Los Banos, Laguna, Philippines
Visiting Research Fellow - at IRRI, Los Banos, Laguna, Philippines
Senior Research Fellow (CSIR)- Indian Institute of Rice Research, Hyderabad, India
Junior Research Fellow (CSIR)- Indian Institute of Rice Research, Hyderabad, India
Senior Research Fellow (ICAR) –Indian Institute of Rice Research, Hyderabad, India
Main research topics: Breeding for healthier rice varieties, high beta-carotene, iron and zinc.
Affiliations and expertise
Currently Senior Scientist I-Senior Breeder, IRRI, Los Banos, Laguna, PhilippinesAM
Anca Macovei
Dr. Anca Macovei earned a degree in Biology and a Masters Degree in Plant Genetic Engineering at the ‘Babes-Bolyai’ University in Cluj-Napoca (Romania) followed by a PhD Degree in Genetics and Molecular Biology at the University of Pavia (Italy). Post-doc fellowships were at the International Center for Genetic Engineering and Biotechnology (ICGEB), New Delhi, India and International Rice Research Institute (IRRI), Manila, Philippines Currently, an Assistant Professor at the Department of Biology and Biotechnology (Plant Biotechnology Laboratory), University of Pavia (Italy), main research topics are molecular profiling of seed quality, plant DNA Damage Response (DDR).
Affiliations and expertise
Assistant Professor, Department of Biology and Biotechnology (Plant Biotechnology Laboratory), University of Pavia, ItalyKT
Kurniawan Rudi Trijatmiko
Dr. Kurniawan Rudi Trijatmiko earned a degree in Agriculture at the Gadjah Mada University in Yogyakarta (Indonesia) in 1994. Master Degree in Biotechnology at the Bogor Agricultural University (Indonesia) in 2000. PhD Degree in Plant Sciences at the Wageningen University (Netherlands) in 2005.Following Post-doc fellowships at the International Rice Research Institute (IRRI), Los Baños, Philippines (2009-2011) and Project Scientist at IRRI (2014-2016). Dr.Trijatmiko is currently working as a Scientist I at the Genetic Transformation Laboratory, IRRI with focus on main research topics: engineering rice for high beta-carotene, iron and zinc.
Affiliations and expertise
Scientist I, Genetic Transformation Laboratory, International Rice Research Institute, Los Banos, Laguna, PhilippinesRead Genetic Engineering and Genome Editing for Zinc Biofortification of Rice on ScienceDirect