Gene Probes for Bacteria
- 1st Edition - December 2, 2012
- Imprint: Academic Press
- Editor: Alberto Macario
- Language: English
- Paperback ISBN:9 7 8 - 0 - 1 2 - 4 1 4 3 5 2 - 4
- eBook ISBN:9 7 8 - 0 - 3 2 3 - 1 4 6 6 9 - 2
Gene Probes for Bacteria focuses on the preparation and use of nucleic acid probes for identifying bacteria in clinical specimens and in other samples of practical or scientific… Read more
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Gene Probes for Bacteria focuses on the preparation and use of nucleic acid probes for identifying bacteria in clinical specimens and in other samples of practical or scientific interest. Organized into 17 chapters, this book addresses which nucleic acid probes are available; how and when to utilize them; what to expect in terms of results obtained with their use; and how to prepare probes. Each chapter is composed of comprehensive manuscript covering different microorganisms. A few bacterial species are treated in more than one chapter to provide a multidimensional picture of important microbes, and to compile the knowledge gained from different laboratories using the same or different probes or methods. This reference material will be of great use to professionals, technicians, and R&D directors in universities, federal and state-dependent service and research institutes, and private and industrial laboratories.
Contributors
Preface
Introduction
1 The Use of Nonradioactive DNA Probes for Rapid Diagnosis of Sexually Transmitted Bacterial Infections
I. Introduction
II. Background
III. Results and Discussion
IV. Conclusions
V. Gene Probes versus Antisera and Monoclonal Antibodies
VI. Prospects for the Future
VII. Summary
VIII. Materials and Methods
References
2 Detection of Chlamydia trachomatis with DNA Probes
I. Introduction
II. Background
III. Results and Discussion
IV. Conclusions
V. Gene Probes versus Monoclonal Antibodies
VI. Prospects for the Future
VII. Summary
VIII. Materials and Methods
References
3 Construction of DNA Probes for the Identification of Haemophilus ducreyi
I. Introduction
II. Background
III. Results and Discussion
IV. Conclusions
V. Gene Probes versus Antisera and Monoclonal Antibodies
VI. Prospects for the Future
VII. Summary
VIII. Materials and Methods
References
4 Detection of Diarrheogenic Escherichia coli Using Nucleotide Probes
I. Introduction
II. Background
III. Results and Discussion
IV. Conclusions
V. Gene Probes versus Immunological Assays
VI. Prospects for the Future
VII. Summary
VIII. Materials and Methods
References
5 DNA Probes for Escherichia coli Isolates from Human Extraintestinal Infections
I. Introduction
II. Background
III. Results and Discussion
IV. Conclusions
V. Gene Probes Compared with Other Methods of Detection
VI. Prospects for the Future
VII. Summary
VIII. Materials and Methods
References
6 Identification of Enterotoxigenic Escherichia coli by Colony Hybridization Using Biotinylated LTIh, STIa, and STIb Enterotoxin Probes
I. Introduction
II. Background
III. Results and Discussion
IV. Conclusions
V. Gene Probes versus Antisera and Monoclonal Antibodies
VI. Prospects for the Future
VII. Summary
References
7 Early days in the Use of DNA Probes for Mycobacterium tuberculosis and Mycobacterium avium Complexes
I. Introduction
II. Background
III. Results and Discussion
IV. Conclusions
V. Gene Probes versus Antisera and Monoclonal Antibodies
VI. Prospects for the Future
VII. Summary
VIII. Materials and Methods
References
8 Att Sites, Tox Gene, and Insertion Elements as Tools for the Diagnosis and Molecular Epidemiology of Corynebacterium diphtheriae
I. Introduction
II. Background
III. Results and Discussion
IV. Conclusions and Prospects for the Future
V. Summary
VI. Materials and Methods
References
9 Nucleic Acid Probes for Bacteroides Species
I. Introduction
II. Background
III. Results and Discussion
IV. Conclusions
V. Gene Probes versus Monoclonal Antibodies
VI. Prospects for the Future
VII. Summary
VIII. Materials and Methods
References
10 Nucleic Acid Probes for Campylobacter Species
I. Introduction
II. Background
III. Results and Discussion
IV. Conclusions
V. Gene Probes versus Antisera and Monoclonal Antibodies
VI. Prospects for the Future
VII. Summary
VIII. Materials and Methods
References
11 Detection of Leptospira, Haemophilus, and Campylobacter Using DNA Probes
I. Introduction
II. Leptospirosis
III. Haemophilus
IV. Campylobacter
V. Conclusions
VI. Prospects for the Future
VII. Summary
VIII. Materials and Methods
References
12 Nucleic Acid Probes for the Identification of Salmonella
I. Introduction
II. Background
III. Results and Discussion
IV. Conclusions
V. Gene Probes versus Antisera and Monoclonal Antibodies
VI. Prospects for the Future
VII. Summary
References
13 Gene Probes for Detection of Food-Borne Pathogens
I. Introduction
II. Background
III. Results and Discussion
IV. Conclusions
V. Hybridization Assay versus Conventional Assays
VI. Prospects for the Future
VII. Summary
VIII. Materials and Methods
References
14 The Use of Gene and Antibody Probes in Identification and Enumeration of Rumen Bacterial Species
I. Introduction
II. Background
III. Results and Discussion
IV. Conclusions
V. Gene Probes versus Antisera and Monoclonal Antibodies: Prospects for the Future
VI. Summary
VII. Materials and Methods
References
15 Gene Probe Detection of Human and Cell Culture Mycoplasmas
I. Introduction
II. Background
III. Results and Discussion
IV. Conclusions
V. Gene Probes versus Antisera and Monoclonal Antibodies
VI. Prospects for the Future
VII. Summary
VIII. Materials and Methods
References
16 Detection of TEM β-Lactamase Genes Using DNA Probes
I. Introduction
II. Background
III. Results and Discussion
IV. Conclusions
V. Gene Probes versus Antisera and Monoclonal Antibodies
VI. Prospects for the Future
VII. Summary
VIII. Materials and Methods
References
17 SIA Technology for Probing Microbial Genes
I. Introduction
II. Background
III. Results and Discussion
IV. Conclusions
V. Prospects for the Future
VI. Summary
VII. Materials and Methods
References
Index
- Edition: 1
- Published: December 2, 2012
- Imprint: Academic Press
- Language: English
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