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G Protein-Coupled Receptors
Signaling, Trafficking and Regulation
1st Edition - February 26, 2016
Editor: Arun K. Shukla
Hardback ISBN:9780128035955
9 7 8 - 0 - 1 2 - 8 0 3 5 9 5 - 5
eBook ISBN:9780128036129
9 7 8 - 0 - 1 2 - 8 0 3 6 1 2 - 9
G-Protein-Coupled Receptors: Signaling, Trafficking, and Regulation, a new volume in the Methods in Cell Biology series continues the legacy of this premier serial with quality… Read more
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G-Protein-Coupled Receptors: Signaling, Trafficking, and Regulation, a new volume in the Methods in Cell Biology series continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in G-Protein-Coupled Receptors, and includes sections on such topics signaling, trafficking and regulation.
Covers the increasingly appreciated cell biology field of G-protein-coupled receptors
Includes both established and new technologies
Contributed by experts in the field
Covers topics such as signaling, trafficking, and regulation
Researchers and students in cell, molecular and developmental biology
Series Editors
Preface
Section I: Trafficking, Localization and Imaging of GPCRs
Chapter 1. Localization and signaling of GPCRs in lipid rafts
Introduction
1. Localization of GPCRs in Lipid Rafts
2. GPCR Signaling in Lipid Rafts
Chapter 2. Imaging GPCRs trafficking and signaling with total internal reflection fluorescence microscopy in cultured neurons
1. Image Acquisition
2. Image Analysis
3. Final Comments
Chapter 3. Trafficking of ciliary G protein-coupled receptors
Introduction
1. Rhodopsin as a Ciliary GPCR
2. Ciliary GPCRs in Other Neurons
3. Olfactory Sensory Neuron Ciliary GPCRs
4. Dynamic Trafficking of Ciliary GPCRs
Conclusions and Future Directions
Chapter 4. Single-molecule resolution of G protein-coupled receptor (GPCR) complexes
Introduction
1. Imaging Methodologies to Study Single GPCR Molecules
2. Methods for Photoactivated Dye-Localization Microscopy
Conclusions and Perspectives
Chapter 5. Quantification of the mRNA expression of G protein-coupled receptors in human adipose tissue
Introduction
1. Materials
2. Methods
Notes
Section II: Signaling and Regulation of GPCRs
Chapter 6. Studying the regulation of endosomal cAMP production in GPCR signaling
Introduction
1. Materials
2. Experimental Procedures
3. Discussion
Chapter 7. Olfactory receptor signaling
Introduction
1. Specificity
2. Dimerization
3. Perception
4. Olfactory Transduction
5. Mechanisms of Odorant Adaptation
6. Perspectives
Chapter 8. Assessing Smoothened-mediated Hedgehog signaling in zebrafish
Introduction
1. Analysis of Smoothened-mediated Hh Signaling in Zebrafish
Summary
Chapter 9. GPCRs and actin–cytoskeleton dynamics
Introduction
1. GPCRs and G Proteins
2. Actin–cytoskeleton Dynamics
3. GPCR/G Protein Signaling and Actin Polymerization
4. Cellular Processes Controlled by Actin–cytoskeleton Rearrangements Induced by GPCRs
5. The Cross Talk of GPCR Signaling with the Homeostatic Hippo and TGF-β Signaling Pathways
6. Protocols to Evaluate Actin–cytoskeleton Rearrangements
7. Method to Evaluate the Actin–Cytoskeleton Dynamics Modulated by GPCR Signaling in Hepatic Cells
8. Protocols for IF Assays
Conclusions
Section III: Cellular Assays for GPCRs
Chapter 10. GPCR-radioligand binding assays
1. Types of Binding Assays
2. Components of Binding Assays—Considerations for GPCR Binding Assay Design
Abbreviations
Chapter 11. Tracking GPCR biosynthesis and degradation using a nonradioactive pulse chase methodology
Introduction
1. Materials Required
2. Basic Methodology
3. General Workflow of Experiments
4. Results
5. Discussion
Chapter 12. Tango assay for ligand-induced GPCR–β-arrestin2 interaction: Application in drug discovery
1. Background and Rationale
2. Various β-Arrestin Recruitment Assays
3. Principle of Tango Assay
4. Reagents and Methods
5. Data Analysis
6. Results and Data Interpretation
7. Summary and Future Perspectives
Chapter 13. Resonance Energy Transfer-Based Approaches to Study GPCRs
Introduction
1. RET Approaches
2. Ligand Binding
3. Ligand-Induced Conformational Changes
4. GPCR–G Protein Interaction
5. Second Messenger Production
6. β-Arrestin Recruitment
7. Receptor Internalization
8. Receptor Oligomerization
9. Summary and Outlook
Chapter 14. Quantitative analysis of G-protein-coupled receptor internalization using DnaE intein-based assay
Introduction
1. Materials and Methods
2. Discussion
Summary
Chapter 15. Cellular and subcellular context determine outputs from signaling biosensors
Introduction and Rationale
1. Materials and Instrumentation Required
2. Basic Methodology
3. Experimental Strategies
4. Discussion
Section IV: Structural and Computational Investigation of GPCRs
Chapter 16. Protease-activated receptors (PARs) in cancer: Novel biased signaling and targets for therapy
Introduction
1. Novel Signaling of PARs Endowing Critical PH-Domain-Binding Motifs
2. PARs and Human Placenta
3. Future Directions
Chapter 17. Computational methods for studying G protein-coupled receptors (GPCRs)
Introduction
1. Prediction of Receptor Structure
2. Studies of Ligand–Receptor Interactions
3. Biased Signaling
4. Allosterism
5. Dimerization
Chapter 18. Comparing Class A GPCRs to bitter taste receptors: Structural motifs, ligand interactions and agonist-to-antagonist ratios
Introduction
1. Sequence Is Less Conserved Than Structure: Sequence Alignments and GPCR Signature Motifs
2. Ligand–Receptor Interactions in TAS2Rs Compared to Class A GPCRs
Conclusion and Outlook
Chapter 19. What can simulations tell us about GPCRs: Integrating the scales
Introduction
1. Multiscale Simulations: Accuracy, Advantages, and Limitations
2. Analyzing the Dynamics of GPCR–Ligand Interactions
3. Can Current Simulation Techniques Allow Us to Study GPCR Activation?
4. The Dynamic Interplay between the Membrane and Receptors
5. GPCR Organization in the Cell Membrane
Conclusions
Volumes in Series
Index
No. of pages: 502
Language: English
Published: February 26, 2016
Imprint: Academic Press
Hardback ISBN: 9780128035955
eBook ISBN: 9780128036129
AS
Arun K. Shukla
Dr. Arun K. Shukla is a world leader in the field of GPCR biology and he is currently a Professor in the Department of Biological Sciences and Bioengineering at the Institute of Technology, Kanpur in India. Dr. Shukla’s research program is focused on understanding the structure, function and regulation of G protein-coupled receptors with a long-term of designing novel therapeutics with minimized side-effects.