Frontiers in Aquaculture Biotechnology

1st Edition - November 26, 2022
Editors: W.S. Lakra, Mukunda Goswami, Vance L. Trudeau
Language: English
Paperback ISBN:
9 7 8 - 0 - 3 2 3 - 9 1 2 4 0 - 2
eBook ISBN:
9 7 8 - 0 - 3 2 3 - 9 9 8 2 1 - 5

Frontiers in Aquaculture Biotechnology presents a broad-spectrum of topics, covering different key aspects of aquaculture. With the rising importance of aquaculture research,… Read more

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Frontiers in Aquaculture Biotechnology presents a broad-spectrum of topics, covering different key aspects of aquaculture. With the rising importance of aquaculture research, evidence-based information is integral in advancing this field. This book provides a solid resource of information on DNA barcoding for fish species authentication and seafood labelling and cell culture, including stem cell culture, in vitro research using fish cell lines such as in vitro fish meat, reproductive biotechnology, including surrogate technology, gene editing and genetically modified aquaculture species, biofloc technology, and omic technologies such as proteomics, artificial intelligence and biobanking.

This book will be a valuable resource to students, researchers a nd entrepreneurs interested in a better understanding of this emerging field of aquaculture.

Cover image
Title page
Table of Contents
Copyright
List of contributors
Chapter 1. Biotechnology in modern aquaculture: Innovations, advancements, and challenges
1. Introduction
2. Chromosome manipulations
3. Genomics in aquaculture
4. Proteomics
5. Gene editing
6. DNA barcoding
7. Fish cell and stem cell culture
8. Vaccines in aquaculture
9. Biofloc/bioremediation
10. Biobank, cell line repository, and state fish
11. Concept and declaration of State Fish
12. Conclusion
Chapter 2. Opportunities and challenges in aquaculture biotechnology
1. Introduction
2. Genetic engineering using gene editing tools
3. Biotechnology in fish breeding
4. Transgenesis
5. Chromosome engineering
6. Cryopreservation of gametes or gene banking
7. Optimized feed and effective health protection
8. Traceability, residue analysis, and value chain in aquaculture
9. General challenges
10. Other challenges
11. Conclusion
Chapter 3. Genetically improved aquaculture species in Bangladesh: advances and challenges
1. Introduction
2. Breeding plans and genetic stock improvement of carp species
3. Breeding plans and generic stock improvement of tilapia species
4. Impacts of genetically improved aquaculture strains in aquaculture
5. Major strategies, challenges, and way forward
6. Conclusions
Chapter 4. Application of molecular markers in aquaculture
1. Introduction
2. Molecular markers in aquaculture
3. Molecular markers in aquatic species
4. Future prospects of using DNA markers
5. Conclusions
Chapter 5. An update on induced breeding methods in fish aquaculture and scope for new potential techniques
1. Introduction
2. Need for induced breeding in fish aquaculture
3. Historical developments in induced breeding methods
4. Fish gonadotropins and gonadotropin-based techniques
5. Fish GnRHs and GnRH-based techniques
6. Some common inducing agents used in fish aquaculture
7. Kisspeptins and potential role as an induced breeding agent
8. Neurohypophysial peptides and potential role as inducing agents
9. Conclusions and future perspectives
10. Appendix
Chapter 6. Biofloc technology in aquaculture
1. Introduction
2. Basic concepts of biofloc technology
3. Operational parameters to optimize biofloc systems
4. Benefits of biofloc production systems
5. Recent advances in biofloc research
6. Perspectives
7. Conclusions
Chapter 7. Monosex population in aquaculture
1. Introduction
2. Sex determination in fish
3. Methods for production of monosex population
4. Current status of monosex production technology in commercially important aquaculture species
5. Conclusion
Chapter 8. Applications of cell lines and cell culture systems of finfish and shellfishes to attain sustainability in aquaculture
1. Introduction
2. Isolation and study of viral pathogens
3. Cytopathic effects
4. Primary isolation of viruses
5. Method of extraction of virus from tissue
6. The absolute requirements
7. Fish cell lines for the study of intracellular bacterial pathogens
8. Fish cell lines for virus vaccine preparation
9. Fish cell lines for developing antiviral chemotherapy
10. Fish nutrition and metabolism with respect to feed formulation
11. Aquaculture environment quality assessment for sustainable aquaculture production
12. Embryonic stem cell and transgenesis
13. Organoid culture of fish pituitary and tissue engineering
14. Crustacean cell lines
15. Molluscan cell lines
16. Conclusion
Chapter 9. An ocean of opportunity: challenges and opportunities for developing cultivated seafood
1. Unique advantages and challenges
Chapter 10. Recent advances in fish disease diagnosis, therapeutics, and vaccine development
1. Introduction
2. Disease problems in fish culture
3. Disease diagnostic techniques
4. Preventive and control measures
5. Therapeutics in fish disease management
6. Vaccines for fish diseases
7. General principles of fish vaccination
8. Recent advances in vaccine developments
9. Methods of vaccine delivery
10. Nanovaccines—nanoparticle-based vaccine delivery
11. Vaccination strategies
12. Conclusion
Chapter 11. Principles of genome editing and its applications in fisheries
1. Introduction
2. Genome editing technologies
3. Genome editing in aquaculture: an up-to-date status
4. Harnessing causative variants of quantitative trait locus
5. Mimicking beneficial genes/alleles from unlike species or strains by genome editing
6. Creating new variants from scratch based on trait knowledge
7. Challenges of applying genome editing
8. Commercialized CRISPR-edited fish
9. Concluding remarks
Chapter 12. Ultraviolet-B radiation, a potential threat to the aquatic organisms, and its mitigation
1. Introduction
2. Larval survival rate
3. Impact of UV-B radiation on the eyes, gills, and skin
4. Physiological responses of fish
5. Mitigation of UV-B radiation
Chapter 13. Applications of biotechnology in aquaculture nutrition and fisheries
1. Introduction
2. Biotechnology in aquaculture production
3. Biotechnology in fish health management
4. Biotechnology in fish breeding
5. Biotechnology in water quality management
6. Conclusion
Chapter 14. Fish DNA barcoding: advances and challenges
1. Introduction
2. An overview on barcoding project
3. The fish barcode of life campaign
4. Mitochondrial DNA cytochrome oxidase 1 marker
5. DNA barcoding as a fish identification tool
6. Commonly used primers in DNA barcoding of ichthyofauna taxa
7. Advances in fish DNA barcoding
8. Challenges facing fish DNA barcoding
9. Conclusions
Chapter 15. Recent advances in marine biotechnology
1. Introduction and background
2. Evolution of marine biotechnology and marine biodiscovery
3. Biotechnological process
4. Marine metagenomic and transgenic approaches
5. Biotechnological advances to extend the use of ocean bioresources for industrial applications
6. Seaweeds and products: valuable sources of high-value compounds and nutraceutical products
7. Marine-derived pharmaceuticals
8. Bioremediation
9. Role of marine biotechnology in biofouling
10. Applications of marine biotechnology
11. Marine biotechnological conservation
12. In vitro fish flesh production system
13. Publications and patents in the subject area of marine biotechnology
14. Leading countries undertaking marine biotechnological research
15. Marine microorganism: a potential biofactory to derive high-value compounds
16. Marine biotechnology and marine population dynamics
17. Advances in marine biotechnology in renewable energy sector
18. Marine biomaterials and nanoparticle conjugate from marine source: promising opportunities in clinical applications
19. Conclusions
Chapter 16. Seaweed biotechnology implications to aquaculture
1. Introduction
2. Protoplast isolation and fusion
3. Chemicals
4. Chimerism
5. Grafting
6. Haploid cell/spore culture
7. Parthenocarpy
8. Genetic transformation
9. Genetic marker
10. Algal breeding
11. Tissue culture/micropropagation
12. Way forward
Chapter 17. Transcriptome analysis: implication in characterization of genes involved in biosynthesis of economically valuable products from seaweeds
1. Introduction
2. Polysaccharide biosynthesis
3. Pigments and secondary metabolite synthesis
4. Gap area
5. Conclusion and future perspective
Chapter 18. Proteomic approaches and their applications in salmonid health research
1. Introduction
2. Proteomic approaches in salmonid fish health research
3. Conclusion
Chapter 19. Proteo-metabolomic technologies in context of fishery/aquacultural research and applications
1. Introduction
2. Basic terminologies related to proteomics and metabolomics
3. Proteomic and metabolomic workflow
4. Techniques/approaches used for proteomic analysis
5. Current proteomic and metabolomic studies for fishery/aquaculture and authors' perspective
6. Conclusions
Chapter 20. Biobank and genetic conservation of aquatic resources
1. Introduction
2. Conservation strategies
3. Mode of conserving fish germplasm
4. Management of declining population
5. Management of endangered species
6. Conservation strategies
7. Live germplasm resource centers
8. Cryobank
9. Sperm preservation
10. Oocyte and embryo preservation
11. Tissue repository
12. DNA bank
13. Web-based tools
14. Fish cell line repository
15. Initiatives of ICAR-NBFGR for fish genetic resource conservation through biobank
16. Future perspectives
Index

W.S. Lakra

Dr. W. S. Lakra, D. Sc. has over 30 years of experience in fisheries and aquaculture research and management with a specialization in genetics, biotechnology, aquaculture and biodiversity conservation. He is a former Director of ICAR-Central Institute of Fisheries Education, Mumbai and National Bureau of Fish Genetic Resources, Lucknow. He is an internationally acclaimed scientist and has made outstanding contributions in the development of molecular markers including DNA barcodes and cell culture systems from major aquaculture species for biodiversity conservation and biotechnological applications. Dr. Lakra is a Fellow of National Academy of Sciences, India and National Academy of Agricultural Sciences. He has many prestigious awards to his credit and authored more than 350 scientific publications including several books. His name has also figured among the Top 2% most influential scientists worldwide based on the Scopus publications impact as per the report published by the Stanford University, USA recently.

Affiliations and expertise

Ex-Vice Chancellor, ICAR-CIFE, Mumbai, India

Mukunda Goswami

Dr. Mukunda Goswami is presently working as a Principal Scientist at ICAR-Central Institute of Fisheries Education (CIFE), Mumbai. He did his PG and Ph. D. from CIFE, Mumbai and Post-Doctoral research from University of Ottawa, Canada. Dr. Goswami previously worked at ICAR-NBFGR, Lucknow as a Senior Scientist where he was actively involved in fish molecular and biotechnological research including cell culture and DNA Barcoding. He has developed and characterized many fish cell lines including a National Repository of Fish Cell Lines in India. He has also delivered several lectures on fish cell line development and culture techniques in addition to his regular teaching academic courses for PG and Ph. D. students . Presently, he is focusing on whole cut cell-based/cultivated seafood. He has published more than 70 research papers in high impact factor international journals. He is the recipient of many national and international fellowships and awards.

Affiliations and expertise

Central Institute of Fisheries Education, Indian Council of Agricultural Research, India

Vance L. Trudeau

Dr. Trudeau is known worldwide for his work on the neurobiology of reproduction and growth using fish (and frogs) as models. He has ~ 13 years of interdisciplinary research. He is President of a Scientific Society, International Symposium on Fish Endocrinology, serves as an editorial board member on several scientific journals on Fish Endocrinology. He has published hundreds of articles and supervised over ~150 thesis / grad students.

Affiliations and expertise

University Research Chair in Neuroendocrinology, Faculty of Science, Department of Biology, Ottawa, Canada

Life Sciences

Physical Sciences & Engineering

Social Sciences & Humanities

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Frontiers in Aquaculture Biotechnology

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W.S. Lakra

Mukunda Goswami

Vance L. Trudeau