Fluorine Metabolism, Transport and Enzymatic Chemistry
- 1st Edition, Volume 696 - April 19, 2024
- Imprint: Academic Press
- Editor: Randy Stockbridge
- Language: English
- Hardback ISBN:9 7 8 - 0 - 4 4 3 - 2 3 6 4 3 - 3
- eBook ISBN:9 7 8 - 0 - 4 4 3 - 2 3 6 4 4 - 0
Fluorine Metabolism, Transport and Enzymatic Chemistry, Volume 696 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting in… Read more
Purchase options
Institutional subscription on ScienceDirect
Request a sales quoteFluorine Metabolism, Transport and Enzymatic Chemistry, Volume 696 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting topics on Fluorine Metabolism, Transport, and Enzymatic Chemistry. Chapters in this new release include Determination of fluoride sensitivity and resistance in oral biofilm models, NMR methods to detect fluoride binding and transport by membrane proteins, Biotransformation of fluorinated drugs and xenobiotics by the model fungus Cunninghamella elegans, Measurement of fluoride efflux from lipid vesicles and cells, Defluorination of PFAS by Acidimicrobium sp. A6 and Potential Applications for Remediation, and more.
Additional chapters cover Computational approaches to investigate F- binding, selectivity and transport in F- exporters, Defluorination as a key trait to gauge the biodegradability of fluorinated pollutants: challenges and opportunities, Patch-Clamp Electrophysiology of Fluoride Channels in Saccharomyces cerevisiae, and Synthesis of fluorophenylalanine-encoded proteins in HEK cells.
Additional chapters cover Computational approaches to investigate F- binding, selectivity and transport in F- exporters, Defluorination as a key trait to gauge the biodegradability of fluorinated pollutants: challenges and opportunities, Patch-Clamp Electrophysiology of Fluoride Channels in Saccharomyces cerevisiae, and Synthesis of fluorophenylalanine-encoded proteins in HEK cells.
- Provides the authority and expertise of leading contributors from an international board of authors
- Presents the latest release in Methods in Enzymology series
- Updated release includes the latest information on Fluorine Metabolism, Transport, and Enzymatic Chemistry
Academic, government and industrial sectors
- Cover image
- Title page
- Table of Contents
- Series Page
- Copyright
- Contributors
- Preface
- Section 1 Fluoride detection, transport and resistance
- Chapter One: Electrophysiology of fluoride channels in the yeasts Saccharomyces cerevisiae and Candida albicans
- Abstract
- 1 Introduction
- 2 Preparation of S. cerevisiae and C. albicans spheroplasts for patch-clamping
- 3 Patch recording on S. cerevisiae and C. albicans
- 4 Summary
- Acknowledgments
- References
- Chapter Two: NMR methods to detect fluoride binding and transport by membrane proteins
- Abstract
- 1 Introduction
- 2 Sample preparation
- 3 19F transport assay by solution NMR
- 4 Fluoride binding in fluoride transporters detected by solid-state NMR
- 5 Conclusion
- Acknowledgments
- References
- Chapter Three: Lanthanum-fluoride electrode-based methods to monitor fluoride transport in cells and reconstituted lipid vesicles
- Abstract
- 1 Introduction
- 2 Working with fluoride: safety measures and experimental considerations
- 3 The fluoride electrode
- 4 An electrode-based assay to measure fluoride efflux from proteoliposomes
- 5 An electrode-based assay to measure fluoride influx into cells
- 6 Conclusions
- Acknowledgment
- References
- Chapter Four: Microwell fluoride assay screening for enzymatic defluorination
- Abstract
- 1 Introduction
- 2 General overview of method
- 3 Method details
- 4 Experimental examples
- 5 Constraints and interferences
- 6 High-throughput screening
- 7 Summary and conclusions
- Acknowledgments
- References
- Chapter Five: Direct fluoride monitoring using a fluorogenic RNA-based biosensor
- Abstract
- 1 Introduction
- 2 General method
- 3 Biosensor production and purification
- 4 Free fluoride detection in simple or complex media
- 5 Summary and conclusions
- Acknowledgments
- References
- Chapter Six: Computational approaches to investigate fluoride binding, selectivity and transport across the membrane
- Abstract
- 1 Background
- 2 CLCF: a case study in umbrella sampling
- 3 Fluc: a case study in computational electrophysiology
- 4 Summary and conclusions
- Data availability
- References
- Chapter Seven: Measurement and analysis of microbial fluoride resistance in dental biofilm models
- Abstract
- 1 Introduction
- 2 Preparation of media and solutions
- 3 Three-species biofilm on polystyrene stalks
- 4 Three-species biofilm on hydroxyapatite (HA) discs in a feast-and-famine model
- 5 Case study: Preparation and analysis of fluoride-sensitive S. mutans in three-species biofilm
- 6 Summary
- Acknowledgements
- References
- Section 2 Enzymatic fluorination
- Chapter Eight: Preparation, assay, and application of 4-fluorothreonine transaldolase from Streptomyces sp. MA37 for β-hydroxyl amino acid derivatives
- Abstract
- 1 Introduction
- 2 Preparation and assay of FTaseMA
- 3 Chemoenzymatic synthesis of βHAAs
- 4 Summary and conclusions
- Acknowledgments
- References
- Chapter Nine: Synthesis of fluorinated amino acids by low-specificity, promiscuous aldolases coupled to in situ fluorodonor generation
- Abstract
- 1 Introduction
- 2 Production and purification of His6×-tagged LTAs
- 3 In situ generation of 2-fluoroacetaldehyde from 2-fluoroethanol
- 4 In vitro production of 4-F-Thr via aldol addition
- 5 Cleavage of 4F-Thr via retro-aldol reaction
- 6 Amino acid quantification by reverse-phase HPLC
- 7 Growth coupled assay for probing in vivo enzyme activity
- 8 Summary
- Acknowledgments
- References
- Chapter Ten: Radical fluorine transfer catalysed by an engineered nonheme iron enzyme
- Abstract
- 1 Introduction
- 2 Materials
- 3 Protocols
- 4 Summary
- Acknowledgments
- References
- Section 3 Biodegradation of organofluorine compounds
- Chapter Eleven: Biotransformation of fluorinated drugs and xenobiotics by the model fungus Cunninghamella elegans
- Abstract
- 1 Introduction
- 2 Fungal cultivation
- 3 Biotransformation of fluorinated xenobiotics
- 4 Analysis of metabolites by 19F NMR spectroscopy
- 5 Analysis of metabolites by gas chromatography–mass spectrometry
- 6 Analysis by metabolites by liquid chromatography–mass spectrometry (LC–MS)
- 7 Fluoride ion measurement
- 8 Heterologous expression of C. elegans CYPs in Pichia pastoris
- 9 Summary and conclusions
- References
- Chapter Twelve: Defluorination of PFAS by Acidimicrobium sp. strain A6 and potential applications for remediation
- Abstract
- 1 Introduction
- 2 Methods
- 3 Defluorination of PFAS by Acidimicrobium sp. strain A6
- 4 Biodegradation of PFAS in biosolids via amendment with Acidimicrobium sp. strain A6 and ferric iron
- 5 Overcoming the challenge of supplying a solid Fe(III) source in selected applications
- 6 Conclusions
- Acknowledgements
- References
- Chapter Thirteen: Defluorination as the key trait to gauge the biodegradability of fluorinated pollutants in environmental microbial communities
- Abstract
- 1 Introduction
- 2 Bioprospecting for defluorinating microorganisms and microbial communities
- 3 Community functionality at the interface of (un)cultivability—the role of artificial selection
- 4 Fingerprinting microbial defluorination with fluoride as analytical proxy
- 5 Final considerations
- Acknowledgments
- References
- Section 4 Fluorine as a biochemical probe
- Chapter Fourteen: Expression and purification of fluorinated proteins from mammalian suspension culture
- Abstract
- 1 Introduction
- 2 Materials and reagents
- 3 Molecular biology
- 4 Test encoding of ncAA into target in adherent cells
- 5 Starting and scaling HEK293S cells in suspension
- 6 Making Polyethylenimine (PEI)
- 7 Testing PEI:DNA ratios
- 8 Express proteins with ncAA on large scale
- 9 Summary and conclusions
- Acknowledgements and funding
- References
- Edition: 1
- Volume: 696
- Published: April 19, 2024
- Imprint: Academic Press
- No. of pages: 412
- Language: English
- Hardback ISBN: 9780443236433
- eBook ISBN: 9780443236440
RS
Randy Stockbridge
Randy B. Stockbridge is an Associate Professor in the Department of Molecular, Cellular, and Developmental Biology at the University of Michigan. She also holds an appointment in the Program in Biophysics. She received her Ph.D. in Biochemistry and Biophysics in 2010 from the University of North Carolina, Chapel Hill, where she conducted mechanistic enzymology research in the laboratory of Dr. Richard Wolfenden. She carried out her postdoctoral studies in the lab of Dr. Christopher Miller at Brandeis University, where she first discovered and characterized new families of microbial fluoride export proteins. Her research lab at the University of Michigan applies structural biology, biochemistry, and biophysical approaches to understand microbial membrane physiologies, including fluoride resistance, antiseptic resistance, and metabolite transport. She serves on the Board of Reviewing Editors for the journal eLife and the editorial board for the Journal of General Physiology. She is also active in undergraduate education at the University of Michigan. She teaches Introductory Biochemistry to approximately 450 students each year, along with specialty courses on macromolecular structure. She has been awarded the Paul F. Cranefield Award from the Society of General Physiologists, and the Margaret Oakley Dayhoff Award from the Biophysical Society, an NSF CAREER award, and research awards from the Burroughs Wellcome Fund and Alfred P. Sloan Foundation.
Affiliations and expertise
Associate Professor, Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI, USARead Fluorine Metabolism, Transport and Enzymatic Chemistry on ScienceDirect