Fibronectin

Contributors

Preface

Primary Structure of Fibronectin

I. Introduction

II. Primary Structure

III. Genomic Structure

References

Note Added in Proof

Physical Properties of Fibronectin

I. Introduction

II. Electron Microscopy

III. Shape in Solution

IV. Spectroscopic Properties

V. Conformational Stability

VI. Correlation Between Conformational and Sequence Information

References

Fibronectin Domains and Receptors

I. Introduction

II. Functional Activities of Fibronectin

III. Characterization of Domains in the Intact Molecule

IV. Cell Interaction Sites

V. Collagen-Binding Site

VI. Fibrin-Binding Domains

VII. Glycosaminoglycan-Binding Domains

VIII. Other Domains

IX. Fibronectin Receptor

X. Future Prospects

References

Fibronectin and the Pericellular Matrix

I. Introduction

II. Pericellular Fibronectin Fibers

III. Association of the Matrix with the Cell Surface

IV. Isolation and Composition of the Pericellular Matrix

V. Cellular Effects of Isolated Matrices

VI. Proteoglycans in the Pericellular Fibronectin-Containing Matrix

References

Fibronectin-Cytoskeleton Relationships

I. Introduction

II. The Fibronexus in Vitro

III. In Vivo Studies of Fibronectin-Cytoskeleton Associations

IV. Summary and Future Directions

References

The Assembly of the Fibronectin Matrix in Cultured Human Fibroblast Cells

I. Introduction

II. Kinetics of Matrix Assembly

III. Identification of a Cell-Binding Site for Matrix Assembly on the Fibronectin Molecule

IV. Mechanism of Disulfide-Bonded Multimer Formation

V. Mechanism for Matrix Assembly

VI. Cell Surface Receptors for Fibronectin

References

Roles of Fibronectins in Embryogenesis

I. Introduction

II. Possible Involvement of Fibronectins in Primary Processes

III. Requirement for Fibronectins During Embryonic Cell Migration

IV. Concluding Remarks

References

Fibronectin in Wound Healing

I. Introduction

II. Outline of Events in a Simple Incisional Skin Wound

III. Provisional Matrix

IV. Fibroblasts

V. Epithelium

VI. Blood Vessels

VII. Macrophages

VIII. Other Cells, Other Wounds

IX. Evolutionary Aspects of Wound Healing

X. Defective Wound Healing

XI. Control Mechanisms

XII. Can Exogenous Fibronectin Accelerate Wound Healing?

References

Fibronectin and Malignant Transformation

I. Introduction

II. Fibronectin and Transformation of Fibroblastic Cells

III. Fibronectin and Malignant Epithelial Cells

IV. Fibronectin Production and Tumor Cell Classification

V. Fibronectin and Malignant Growth in Vivo

VI. Fibronectin and Cell Anchorage in Transformation

VII. Proteolysis of Fibronectin and Malignancy

VIII. Focal Proteolysis

IX. Concluding Remarks

References

Fibronectin: A Contender in Platelet Adhesive Functions

I. Introduction

II. Role of Fibronectin in Platelet Function

III. Localization of Fibronectin

IV. Binding of Fibronectin to Platelet(s)

V. Concluding Remarks

References

Interactions of Pathogenic Microorganisms with Fibronectin

I. Introduction

II. Fibronectin-Binding Microorganisms

III. Binding Sites in Fibronectin for Different Microorganisms

IV. Microbial Fibronectin Receptors

V. Regulation of Fibronectin Receptor Expression

VI. Implications of Microbial Fibronectin Binding

VII. Microbial Binding of Other Matrix Components

References

Fibronectin and the Kidney

I. Introduction

II. Localization of Fibronectin

III. Role of Fibronectin in Differentiation of the Nephron

IV. Role of Fibronectin in Kidney Culture

V. Role of Fibronectin in Kidney Pathology

VI. Pathology of Animals Experimentally Immunized with Fibronectin

VII. Summary

VIII. Future Directions

References

Role of Fibronectin in Rheumatic Diseases

I. Introduction

II. Fibronectin in Rheumatoid Arthritis (RA)

III. Effects of Fibronectin on the Function of Cell Types Found in Inflammatory Synovium

IV. Fibronectin and Cartilage

V. Interactions among Fibronectin and Other Macromolecules in Synovial Fluid

VI. Other Rheumatic Diseases

VII. Conclusion

References

Fibronectin in the Lung

I. Introduction

II. Localization of Fibronectin in the Lung

III. Fibronectin Synthesis by Lung Cells

IV. Fibronectin in the Diseased Lung

V. Potential Roles of Fibronectin in the Lung

References

Kinetics of Plasma Fibronectin: Relationship to Phagocytic Function and Lung Vascular Integrity

I. Introduction

II. The Reticuloendothelial Systemic Host Defense System

III. Reticuloendothelial Particle Clearance and Plasma Fibronectin Depletion

IV. Reticuloendothelial Function and Resistance to Trauma

V. Opsonic α2 Surface-Binding (SB) Glycoprotein and Plasma Fibronectin

VI. Cell Surface and Tissue Fibronectin

VII. Domain Organization of Fibronectin (Structure-Function Relationship)

VIII. Pulmonary Failure During Sepsis Posttrauma

IX. Multiple Organ Failure and Plasma Fibronectin Deficiency

X. Reversal of Plasma Fibronectin Deficiency in Patients with Cryoprecipitate or Purified Human Plasma Fibronectin

XI. Plasma Fibronectin and Lung Vascular Permeability with Sepsis

XII. Kinetics of Plasma Fibronectin

XIII. Conclusions

XIV. Forecast for Future Developments

References

Preparation of Fibronectin for Therapeutic Administration

I. General Remarks

II. Assay of Fibronectin Opsonic Activity in Vitro

III. Purification

IV. Characterization of Purified Fibronectin

V Concluding Remarks

References

Index