Extracellular Vesicles for Therapeutic and Diagnostic Applications
- 1st Edition - January 20, 2025
- Imprint: Elsevier
- Editors: Anand Krishnan, Vadivalagan Chithravel, Prakash Gangadaran, Sathish Muthu, Ben Peacock
- Language: English
- Paperback ISBN:9 7 8 - 0 - 4 4 3 - 2 3 8 9 1 - 8
- eBook ISBN:9 7 8 - 0 - 4 4 3 - 2 3 8 9 2 - 5
Extracellular Vesicles for Therapeutic and Diagnostic Applications provides an introduction to exosomes and extracellular vesicles, including their unique properties and character… Read more
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Request a sales quoteExtracellular Vesicles for Therapeutic and Diagnostic Applications provides an introduction to exosomes and extracellular vesicles, including their unique properties and characterization, before detailing the most-utilized isolation, purification, and surface engineering techniques for their preparation as therapeutic and diagnostic agents. This book reviews recent developments in interdisciplinary research on exosome structure, properties, and engineering approaches as well as their use in diagnosis and treatment of a variety of illnesses such as cancer, tuberculosis, Alzheimer's disease, ophthalmic diseases, and others. Extracellular Vesicles for Therapeutic and Diagnostic Applications is an important reference for those interested in the development and application of exosomes and extracellular vesicles as biomaterials for us in the diagnosis and treatment of disease.
- Provides a step-by-step overview of exosomes and extracellular vesicles and how they can be engineered with biomaterials for use in diagnostics and treatment
- Covers a broad range of biomedical applications, including cancer management, bone diseases, vaccine delivery, and many more
- Offers an interdisciplinary perspective on extracellular vesicles as biomaterials for therapeutic and diagnostic applications that will appeal to a wide audience
Researchers and postgraduate students in the fields of materials science, biochemistry, and translational medicine
- Title of Book
- Cover image
- Title page
- Table of Contents
- Copyright
- List of contributors
- Preface
- Chapter 1. Isolation and purification of exosome and other extracellular vesicles
- Abstract
- 1.1 Introduction
- 1.2 Centrifugation-based isolation of extracellular vesicles
- 1.3 Affinity-based EV isolation
- 1.4 Microfluidics-based EV isolation
- 1.5 Conclusion
- Conflict of interests
- Author’s contributions
- References
- Chapter 2. Overview of the characterization of extracellular vesicles
- Abstract
- 2.1 Background on extracellular vesicles
- 2.2 Classification of extracellular vesicles
- 2.3 Cargo compositions
- 2.4 Importance of characterizing extracellular vesicles
- 2.5 EV isolation techniques
- 2.6 Physical characterization of extracellular vesicles
- 2.7 Molecular profiling of extracellular vesicles
- 2.8 Conclusion
- Funding information
- Conflict of interest statement
- Data availability statement
- References
- Chapter 3. Extracellular vesicles for therapeutic and diagnostic applications
- Abstract
- Graphical abstract
- 3.1 Introduction
- 3.2 Features and modification in extracellular vesicle
- 3.3 Different detection mechanisms of exosomes
- 3.4 Various disease-based extracellular vesicles as diagnostic biomarkers
- 3.5 Clinical prospective and challenges
- 3.6 Concluding remarks
- Abbreviations
- References
- Chapter 4. Preclinical evidence of extracellular vesicles for therapeutic and diagnostic applications
- Abstract
- 4.1 Introduction
- 4.2 Therapeutic and diagnostic applications in preclinical models
- 4.3 Cancer
- 4.4 Cardiovascular diseases and disorders
- 4.5 Neurodegenerative diseases and disorders
- 4.6 Liver diseases and disorders
- 4.7 Kidney diseases and disorders
- 4.8 Pulmonary diseases and disorders
- 4.9 Reproductive diseases and disorders
- 4.10 Wound healing research
- 4.11 Musculoskeletal disorders
- 4.12 Conclusion and prospects
- References
- Chapter 5. Extracellular vesicles as biomarker and therapeutic targets for neurological disorders
- Abstract
- 5.1 Introduction
- 5.2 Extracellular vesicles in the central nervous system
- 5.3 EVs as biomarkers for neurological disorders
- 5.4 EVs as therapeutic target for neurological disorders
- 5.5 Future directions and conclusion
- References
- Chapter 6. Revolutionizing cardiovascular health: exploring the extracellular vesicles
- Abstract
- 6.1 Introduction
- 6.2 The roles of extracellular vesicle contents in inflammation and cardiovascular disease
- 6.3 Extracellular vesicles in cardiovascular disease pathophysiology
- 6.4 Extracellular vesicles as biomarkers for cardiovascular disease diagnosis and prognosis
- 6.5 Extracellular vesicles as therapeutic agents for cardiovascular disease treatment
- 6.6 Extracellular vesicles as drug delivery systems for cardiovascular disease treatment
- 6.7 Conclusion
- References
- Chapter 7. Extracellular vesicles for infectious diseases: a game-changer in infectious disease control
- Abstract
- 7.1 Introduction
- 7.2 Role of EVs in infection
- 7.3 Extracellular vesicles for the treatment of infectious diseases
- 7.4 EVs for prevention of infectious diseases
- 7.5 Challenges and limitations
- 7.6 Conclusion
- References
- Chapter 8. Extracellular vesicles against viral pathogens and infectious diseases
- Abstract
- 8.1 Introduction
- 8.2 Overview on infectious diseases—gaps and prospects
- 8.3 Extracellular vesicles as emerging drug delivery cargoes
- 8.4 Exosomal microenvironment against viral infections and other infectious agents
- 8.5 Extracellular vesicles as biomarkers for pathogens
- 8.6 Extracellular vesicles against emerging and preexisting viral infections
- 8.7 Extracellular vesicles against different infectious agents
- 8.8 Challenges associated with infectious disease therapy and extracellular vesicles
- 8.9 Future perspectives and conclusion
- Acknowledgments
- References
- Chapter 9. Theranostic potential of extracellular vesicles released from mycobacteria and mycobacterium-infected host cells
- Abstract
- 9.1 Introduction
- 9.2 Mycobacterial EV biogenesis
- 9.3 Isolation and purification of mycobacterial extracellular vesicles
- 9.4 Host immunomodulation by mycobacteria-derived extracellular vesicles
- 9.5 Cellular alterations induced by exosomes from mycobacterium-infected hosts
- 9.6 Theranostic application of mycobacteria-derived extracellular vesicles
- 9.7 Theranostic applications of exosomes derived from mycobacterium-infected hosts
- 9.8 Implications of extracellular vesicles in the diagnosis of different TB subtypes
- 9.9 EV-based mimetics
- 9.10 Conclusion
- 9.11 Future prospects
- Abbreviations
- References
- Chapter 10. Extracellular vesicles in reproductive medicines
- Abstract
- 10.1 Introduction
- 10.2 Extracellular vesicles
- References
- Chapter 11. Extracellular vesicles for ophthalmic and dermal ailments
- Abstract
- 11.1 Introduction
- 11.2 Overview of exosomes
- 11.3 Bioengineering and loading of cargo in exosomes
- 11.4 Exosomes as vectors for advanced therapies
- 11.5 Clinical potential of exosomes
- 11.6 Application of exosomes in topical drug delivery
- 11.7 Application of exosomes for ocular drug delivery
- 11.8 Exosomes as bioinspired nanocarriers for RNA delivery: microneedle platform
- 11.9 Biodistribution of exosomes
- 11.10 Potential challenges or risk of exosomes
- 11.11 Conclusion
- Conflict of interest
- References
- Chapter 12. Extracellular vesicles for intervertebral disc regeneration
- Abstract
- 12.1 Introduction
- 12.2 Intervertebral disc degeneration: epidemiology, pathophysiology, and current regenerative treatments
- 12.3 Extracellular vesicles and intervertebral disc regeneration
- 12.4 Inhibition of apoptosis
- 12.5 Maintenance of ECM homeostasis
- 12.6 Antioxidant effects
- 12.7 Antiinflammatory effects
- 12.8 Current issues and future perspectives
- 12.9 Conclusion
- References
- Chapter 13. Extracellular vesicles: unlocking therapeutic potential in regenerative medicine
- Abstract
- 13.1 Introduction
- 13.2 Regenerative medicine
- 13.3 EVs
- References
- Chapter 14. Role of extracellular vesicles in neurodegenerative diseases
- Abstract
- 14.1 Introduction to neurodegenerative diseases
- 14.2 Parkinson’s disease
- 14.3 Extracellular vesicles
- 14.4 Extracellular vesicles as potential biomarkers for Alzheimer’s disease and Parkinson’s disease
- 14.5 Therapeutic potential of EVs for neurodegenerative diseases
- 14.6 Future perspectives
- References
- Chapter 15. Extracellular vesicle: the future personalized, targeted drug therapy towards cancer management
- Abstract
- 15.1 Introductory remarks
- 15.2 Tumor-promoting properties of EVs
- 15.3 The extracellular vesicle role in tumor suppressor pathways
- 15.4 Extracellular vesicles in different stages of cancer
- 15.5 Future aspect and extracellular vesicle as a therapeutic agent
- 15.6 Concluding remark
- Acknowledgment
- Abbreviations
- References
- Chapter 16. Exosome and other extracellular vesicles for bone diseases
- Abstract
- 16.1 Introduction
- 16.2 Exosomal regulation of bone homeostasis
- 16.3 Exosomal-mediated osteoblast differentiation and function
- 16.4 Exosomal regulation of osteoblast
- 16.5 Exosomal regulation of osteoclastogenesis and bone resorption
- 16.6 Exosomal communication within the bone microenvironment
- 16.7 Implications of exosomes in bone diseases
- 16.8 Osteoporosis
- 16.9 Osteoarthritis
- 16.10 Bone metastasis
- 16.11 Fracture healing
- 16.12 Diagnostic and therapeutic opportunities
- 16.13 Clinical evidence
- 16.14 Challenges and future directions
- 16.15 Conclusion
- References
- Chapter 17. In silico techniques, artificial intelligence, and machine learning for enhanced efficacy of extracellular vesicle–based diagnosis and therapeutics
- Abstract
- 17.1 Introduction
- 17.2 Diagnostic role of extracellular vesicles
- 17.3 Therapeutic applications of extracellular vesicles
- 17.4 Role and application of bioinformatic tools in extracellular vesicle design and applications
- 17.5 Roles and applications of artificial intelligence and machine learning
- References
- Chapter 18. Regulatory concerns for exosome- and other extracellular vesicle-based diagnostics and medicine products
- Abstract
- 18.1 Therapeutic potential of EVs and exosomes
- 18.2 Regulatory requirements to be considered in developing EV-based diagnostic and therapeutic applications
- 18.3 Intellectual property and commercialization concerns
- 18.4 Exceptions to compliance with MISEV guidelines
- References
- Index
- Edition: 1
- Published: January 20, 2025
- Imprint: Elsevier
- No. of pages: 572
- Language: English
- Paperback ISBN: 9780443238918
- eBook ISBN: 9780443238925
AK
Anand Krishnan
Dr. Anand Krishnan is a Principal Investigator in the Department of Chemical Pathology at the University of the Free State, South Africa. He leads the Precision Medicine Integrative Nano Diagnostics (P-MIND) laboratories, focusing on the biological functions of extracellular vesicles (EVs) in synthetic biology. His research includes cell surface protein modification, biomarker discovery from liquid biopsies, and the role of circulating biomarkers in disease and stem cell biology. Dr. Krishnan has authored over 150 articles and edited eight books with major publishers. He has received a National Research Foundation rating for Next Generation Researchers in South Africa and was recognized among the Top 2% of influential scientists by Stanford University. He is an active member of the International Society of Extracellular Vesicles, the African Organisation for Research and Training in Cancer, and the American Association of Cancer Research. He also serves as an editor for the eLife journal, focusing on the Africa region.
Affiliations and expertise
Precision Medicine and Integrated Nano-Diagnostics (P-MIND) Research Group, Office of the Dean, Faculty of Health Sciences, University of the Free State, Bloemfontein, South AfricaVC
Vadivalagan Chithravel
Dr. Vadivalagan Chithravel works as a Research Fellow in the Department of Surgery at the University of Michigan, Ann Arbor, specializing in medical science, excluding epidemiology. The Exosome Mediated Signal Transduction Mechanism is his area of expertise. His current work focuses on a glycosylation biomarker associated with an exosome pathway for the physiology of cells with HCC. In addition to being a group director and scientist at the SAARP foundation in India, he has held postdoctoral research fellow positions at Taipei Medical University and China Medical University in Taiwan. He is also an active member of the International Society of Extracellular Vesicles and the American Association of Extracellular Vesicles.
Affiliations and expertise
Department of Surgery, Michigan Medicine, University of Michigan Medical Centre, Ann Arbor, Michigan, USAPG
Prakash Gangadaran
Dr. Prakash Gangadaran is Principal Investigator and FOUR-Brain Korea Post-Doctoral Fellow at the Department of Biomedical Science and Nuclear Medicine, School of Medicine, Kyungpook National University, Daegu, South Korea. He has expertise in utilization of extracellular vesicles in angiogenesis, wound healing, drug delivery, molecular and nuclear imaging. He has published more than 48 research articles in international peer-reviewed journals and has authored 2 book chapters as well as 24 review articles. His research interests include extracellular vesicles, angiogenesis, wound healing and drug delivery, radiotherapies, molecular imaging and nuclear imaging.
Affiliations and expertise
BK21 FOUR KNU Convergence Educational Program of Biomedical Sciences for Creative Future Talents, Department of Biomedical Sciences, School of Medicine, Kyungpook National University, Daegu, Republic of Korea; Department of Nuclear Medicine, School of Medicine, Kyungpook National University, Daegu, Republic of Korea; Cardiovascular Research Institute, Kyungpook National University, Daegu, Republic of Korea.SM
Sathish Muthu
Dr. Sathish Muthu is an Assistant Professor in the Department of Orthopaedics at Government Medical College, Karur, India. He is the Co-Founder and Head of Research at Orthopaedic Research Group, a non-profit research organization dedicated to translational orthopaedic research. He is also a steering committee member of the Indian Stem Cell Study Group. and associate member of Knowledge Forum Degenerative of AO Spine. Despite being a board-certified orthopaedic surgeon, Dr. Muthu holds fellowships in spine surgery, orthopaedic rheumatology, and regenerative medicine. He has published over 275 articles in international peer-reviewed journals and has contributed 5 chapters to books on cartilage injury and regeneration. He is also an Associate Editor at various academic journals like PLOS One, Journal of Clinical Medicine, Indian Journal of Orthopaedics, Journal of Orthopaedic Case Reports. His research focuses on cartilage biology, mesenchymal stem cells, exosomes, degenerative disorders, osteoarthritis, and cartilage regeneration.
Affiliations and expertise
Orthopaedic Research Group, Coimbatore, Tamil Nadu, India Department of Orthopaedics, Government Medical College, Karur, Tamil Nadu, India Department of Biotechnology, Karpagam Academy of Higher Education, Coimbatore, Tamil Nadu, IndiaBP
Ben Peacock
Dr. Ben Peacock is Head of Research at NanoFCM Co., Ltd. He has expertise in exosomal miRNA and protein profiles of EVs from cancer cell lines by RNA-seq and mass spectrometry (ion-trap) with validation by RT-qPCR and further western blotting. In international peer-reviewed journals, he has published more than nine research articles. His research interests include EV research, but he is well established in stromal communication of cancers of the head and neck. Multiple procedures were optimized to maximize EV generation from cell lines, improve concentration and purity of EV isolations, and implement best practices for sample storage.
Affiliations and expertise
Head of Research. NanoFCM Co., Ltd, Nottingham, UKRead Extracellular Vesicles for Therapeutic and Diagnostic Applications on ScienceDirect