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Experimental Models of Infection, Inflammation and Injury

1st Edition - March 31, 2022

Editor: David Montrose

Hardback ISBN:

9 7 8 - 0 - 3 2 3 - 8 9 9 4 5 - 1

eBook ISBN:

9 7 8 - 0 - 3 2 3 - 8 9 9 4 6 - 8

Experimental Models of Infection, Inflammation and Injury, Volume 168 provides step-by-step protocols for scientific researchers to effectively utilize experimental model systems.… Read more

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Experimental Models of Infection, Inflammation and Injury, Volume 168 provides step-by-step protocols for scientific researchers to effectively utilize experimental model systems. Chapters in this new release include Induction and Evaluation of Murine T Cell Transfer Colitis, Modelling acute graft-versus-host disease (aGVHD) in murine bone marrow transplantation (BMT) models with MHC disparity, Mouse models of Graft-Versus-Host Disease, Preclinical Model of Multiple Sclerosis: Methods in Autoimmune Demyelination, Preclinical model of Multiple Sclerosis: focal, chemical or demyelination, Investigating demyelination, efficient remyelination and remyelination failure in organotypic cerebellar slice cultures: workflow and practical tips, and more.

Other notable sections cover Rheumatoid Arthritis: Methods for Two Murine Models, Induction of Pancreatitis in Mice with Susceptibility to Pancreatic Cancer, Small Animal Models of Thermal Injury, Large Animal Models of Thermal Injury, Small animal models of localized heart irradiation, Methods for Induction and Assessment of Intestinal Permeability in Rodent Models of Radiation Injury, and more.

Cover image
Title page
Table of Contents
Copyright
Contributors
Chapter 1: Induction and evaluation of murine colitis induced by T cell transfer
Abstract
1: Introduction
2: Reagents and equipment
3: Harvesting and isolating splenocytes
4: Enrichment of CD4+ T cells
5: Antibody staining of naïve T cells
6: Fluorescence-assisted cell sorting (FACS) of naïve T cells
7: Injecting Rag1−/− mice with CD4+ CD45RBhigh T cells
8: Monitoring clinical disease progression
9: Tissue collection at the terminal time point
10: Disease assessment using harvested tissue
11: Concluding remarks
12: Notes
Acknowledgments
References
Chapter 2: Modeling acute graft-versus-host disease (aGVHD) in murine bone marrow transplantation (BMT) models with MHC disparity
Abstract
1: Introduction
2: Conditioning of recipient mice
3: Preparation of donor bone marrow cells
4: Preparation of splenocytes
5: Allogeneic BMT and clinical and histological GVHD assessment
6: Analysis of transplant engraftment and chimerism
7: The B6 → B6.bm1 BMT model
8: The B6 → B6D2F1 BMT model
9: Concluding remarks
10: Notes
References
Chapter 3: Mouse models of graft-versus-host disease
Abstract
1: Introduction
2: GVHD protocols
3: Notes
4: Summary and future directions
Acknowledgments
Appendix A
References
Chapter 4: Preclinical model of multiple sclerosis: Methods in autoimmune demyelination
Abstract
1: Introduction
2: Active induction of EAE
3: Passive induction of EAE
4: Scoring of EAE disease severity
5: Quantification of demyelination
6: Concluding remarks
References
Chapter 5: Preclinical model of multiple sclerosis: Focal, chemical or viral demyelination
Abstract
1: Introduction
2: Cuprizone
3: Lysolecithin
4: Ethidium Bromide
5: Viral models of demyelination Theiler's murine encephalitis virus (TMEV)
6: Scoring for remyelination
7: Concluding remarks
References
Chapter 6: Investigating demyelination, efficient remyelination and remyelination failure in organotypic cerebellar slice cultures: Workflow and practical tips
Abstract
1: Introduction
2: Materials
3: Preparation
4: Dissection
5: Demyelination-remyelination experiments
6: Concluding remarks
Acknowledgments
References
Chapter 7: Rheumatoid arthritis: Methods for two murine models
Abstract
1: Introduction
2: Collagen induced arthritis
3: TNF induced arthritis
4: General considerations
5: Concluding remarks
6: Notes
Funding
References
Chapter 8: Induction of pancreatitis in mice with susceptibility to pancreatic cancer
Abstract
1: Introduction
2: Materials
3: Methods
4: Notes
Acknowledgment
References
Chapter 9: Small animal models of thermal injury
Abstract
1: Introduction
2: Small animal models in burn studies
3: Small animal models replicating clinical burn pathophysiology
4: Post-burn responses in aged vs young animals
5: Differential effects based on biological sex
6: Scald burn model in rodents
7: Notes
8: Take-home messages
Acknowledgments
References
Chapter 10: Large animal models of thermal injury
Abstract
1: Introduction
2: Large animal models in burn research
3: Large animal models replicating clinical burn pathophysiology
4: Conclusion and perspective on animal models
5: Contact burn model in Yorkshire pigs
Acknowledgments
References
Chapter 11: Small animal models of localized heart irradiation
Abstract
1: Introduction
2: A model of image-guided whole heart irradiation in small experimental animals
3: Common histological assessments of cardiac radiation injury
4: Concluding remarks
5: Notes
Acknowledgments
References
Chapter 12: Methods for induction and assessment of intestinal permeability in rodent models of radiation injury
Abstract
1: Introduction
2: Models of radiation-induced gastrointestinal toxicity
3: Evaluation of intestinal permeability
4: Notes
Acknowledgments
References
Chapter 13: Methods and protocols for translatable rodent models of postsurgical pain
Abstract
1: Introduction
2: Procedures
3: Behavioral outcomes and methods
4: Concluding remarks
5: Notes
References
Chapter 14: Methods and protocols for chemotherapy-induced peripheral neuropathy (CIPN) mouse models using paclitaxel
Abstract
1: Introduction
2: CIPN models
3: Behavioral outcomes and methods
4: Concluding remarks
5: Notes
References
Chapter 15: The use of foodborne infection to evaluate bacterial pathogenesis and host response
Abstract
1: Introduction
2: Foodborne L. monocytogenes infection
3: Enumerating bacterial burden
4: Assessing the host response by flow cytometry
5: Concluding remarks
6: Notes
Acknowledgments
References
Chapter 16: Induction and analysis of systemic C. albicans infections in mice
Abstract
1: Introduction
2: Materials
3: Methods
4: Notes
References
Chapter 17: Methods to evaluate virus-mediated acute lung inflammation
Abstract
1: Introduction
2: Materials
3: Methods
4: Notes
References
Further reading