Experimental Biology with Micro-Organisms

Introduction1 Handling, Culturing and Observing Micro-Organisms Expt. 1.1 Micro-Organisms—Their Variety and Their Habitats Expt. 1.2 Isolating a Pure Culture of Bacteria from a Mixed Population Expt. 1.3 Preparing Stained Preparations of Bacteria Appendix—Microscopy Expt. 1.4 Methods of Sterilization Appendix—Autoclaving2 Nutrition Expt. 2.1 Nutrient Requirements in an Autotrophic Organism—Chlorella Expt. 2.2 Nutrient Requirements in a Heterotrophic Organism—Aspergillus niger Expt. 2.3 Protein Digestion in Bacteria Expt. 2.4 Starch Digestion in Aspergillus niger Expt. 2.5 The Effect of the Vitamin Thiamine on the Growth of Heterotrophic Organisms3 Structure and Life Cycles Expt. 3.1 The Structure of Bacteria Appendix—Measurement Expt. 3.2 The Life Cycle of a Green Alga—Chlorella Expt. 3.3 The Life Cycle of a Slime Mould—Dictyostelium discoideum Expt. 3.4 The Life Cycle of a Non-Mycelial Ascomycete-Yeast Expt. 3.5 The Life Cycle of Mucor hiemalis Expt. 3.6 An Examination of Two Mycelial Ascomycetes-Aspergillus (Eurotium) and Penicillium Expt. 3.7 The Life Cycle of a Basidiomycete—Agaricus bisporus4 Growth Expt. 4.1 The Growth of a Population of a Unicellular Organism—Chlorella Appendix—Hemocytometer Expt. 4.2 Growth of a Filamentous Organism—Mucor hiemalis5 Genetics Expt. 5.1 To Demonstrate Gene Segregation and Crossing over Using an Ascomycete Expt. 5.2 Selecting Bacteria Mutants by Using Antibiotics Expt. 5.3 An Investigation into Biochemical Mutants in the Bread Mold—Neurospora crassa6 Soil Ecology Expt. 6.1 Estimating the Numbers of Micro-Organisms in Soils Expt. 6.2 Cellulose Decomposition by Soil Micro-Organisms Expt. 6.3 Ammonification in Soils Expt. 6.4 Nitrification by Soil Organisms Expt. 6.5 Culturing Anaerobic Organisms from the Soil Expt. 6.6 Culturing Soil Protozoa Expt. 6.7 Culturing Soil Algae7 Ecology Expt. 7.1 Temperature and the Growth of Micro-Organisms Expt. 7.2 The Effect of pH on the Growth of Micro-Organisms Expt. 7.3 The Effect of Osmotic Pressure on the Growth of Micro-Organisms Expt. 7.4 An Examination of Water Supplies for Coliform Organisms Expt. 7.5 The Microbiology of Milk Expt. 7.6 To Investigate the Ability of Fungi to Colonize a Range of Substrates8 Interaction Expt. 8.1 An Investigation into Symbiosis Expt. 8.2 Sclerotinia—A Facultative Plant Parasite Expt. 8.3 An Investigation into Bacterial Viruses—Bacteriophages Expt. 8.4 Nitrogen Fixation by a Symbiotic AssociationIndex