Epigenetics Methods
- 2nd Edition - August 31, 2025
- Imprint: Academic Press
- Editor: Trygve O. Tollefsbol
- Language: English
- Paperback ISBN:9 7 8 - 0 - 4 4 3 - 2 6 7 5 9 - 8
- eBook ISBN:9 7 8 - 0 - 4 4 3 - 2 6 7 6 0 - 4
Epigenetics Methods, Second Edition offers an extensive overview of the tools that enhance translational epigenetics studies. This edition includes updated and revised chapte… Read more
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Request a sales quoteEpigenetics Methods, Second Edition offers an extensive overview of the tools that enhance translational epigenetics studies. This edition includes updated and revised chapters, along with newly added topics such as MALDI-TOF-MS for analyzing DNA methylation and the RIP assay for lncRNA binding. Specialists provide step-by-step guidance on methods used to study various epigenetic mechanisms. The book covers both fundamental and advanced techniques, making it an essential resource for researchers in this dynamic field. In addition to DNA methylation and histone modification techniques, the book also explores chromatin evaluation, enzyme analyses, and non-coding RNAs as epigenetic modulators.
New chapters discuss recent advancements like single-cell epigenomics, epigenetic editing, and computational epigenetics. The volume addresses reproducibility issues and offers consensus-driven methods to unify approaches across experiments and labs. This edition is a vital reference for understanding and employing cutting-edge epigenetics methods.
New chapters discuss recent advancements like single-cell epigenomics, epigenetic editing, and computational epigenetics. The volume addresses reproducibility issues and offers consensus-driven methods to unify approaches across experiments and labs. This edition is a vital reference for understanding and employing cutting-edge epigenetics methods.
- Provides revised and updated chapters, as well as new chapters covering recent developments
- Includes contributions by leading international investigators involved in epigenetic research and clinical and therapeutic application
- Integrates technology and translation with fundamental chapters on epigenetics methods, as well as chapters on more novel and advanced epigenetics methods
- Written at verbal and technical levels that can be understood by scientists and students alike
- Includes chapters on state-of-the-art techniques such as single-cell epigenomics, use of CRISPR/Cas9 for epigenetic editing, and epigenetics methods applied to forensics
Basic scientists and clinical researchers that have an interest in epigenetics
Section I: Overview
1. An Overview of Epigenetics Methods
Section II: DNA Methylation Methods: Gene- or Locus-specific Methylation Analyses
2. Principles of Bisulfite Conversion of DNA and Methylation-specific PCR (MSP) in Biological Research
3. Analysis of DNA Methylation Using Pyrosequencing
4. Matrix-assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF-MS) for Analysis of DNA Methylation
5. Methylation-Specific Droplet Digital PCR (MS-ddPCR) for Detection and Absolute Quantification of Rare Methylated Alleles
Section III: DNA Methylation Methods: Global DNA Methylation and Methylomic Analyses
6. ELISA Analyses of Global Methylation Levels
7. Bisulfite PCR of Repetitive Genomic Sequences
8. The Illumina Infinium Methylation Assay for Genome-wide Methylation Analyses
9. Analysis of Genome-wide Methylation Using Reduced Representation Bisulfite Sequencing (RRBS) Technology
10. Methylated DNA Immunoprecipitation Sequencing (MeDIP-seq): Principles and Applications
11. Multiplex Analyses Using Methylation Sensitive Restriction Enzyme qPCR
12. Advances in Whole Genome Methylomic Sequencing
Section IV: DNA Methylation Methods: Additional Technologies
13. Analyzing DNA Methyltransferase Enzymatic Activity: Basic Methods and Applications
14. Technologies and Applications for the Assessment of 5-Hydroxymethylcytosine
Section V: Histone Modification Methods
15. The Chromatin Immunoprecipitation (ChIP) Assay and ChIP-qPCR
16. Deep Sequencing for Histone Modifications Employing ChIP-seq Analyses
17. ChIP in Series: Sequential ChIP
18. ChIP-exo: A Method to Study Chromatin Structure and Organization at Near-Nucleotide Resolution
19. Monitoring Genome-wide Chromatin Accessibility by Formaldehyde-assisted Isolation of Regulatory Elements Sequencing (FAIRE-seq)
20. ATAC-seq Method Applied to Embryonic Germ Cells and Neural Stem Cells from Mouse: Practical Tips and Modifications
21. Combinatorial ChIP and Bisulfite Sequencing (BisChIP-seq)
Section VI: Epigenetic Methods for Evaluating Chromatin Higher Order
22. 3D DNA FISH for Analyses of Chromatin-Nuclear Architecture
23. Capture Hi-C: Characterization of Chromatin Contacts
Section VII: Evaluation of Chromosomal Proteins
24. Assessment of the Epigenetic Nature of Chromosomal Proteins During DNA Replication and Mitosis Section VIII: Enzyme Analyses of Histone Methylation
25. Assessment of Histone-Modifying Enzymes Involved in Epigenetic Gene Regulation
Section IX: Assessment of Non-Coding RNA as an Epigenetic Modulator
26. Detection and Quantification of MicroRNAs (miRNAs) and High Throughput miRNAs Profiling
27. Binding of Long Noncoding RNAs (lncRNAs) to RNA Binding Proteins Using the RNA Immunoprecipitation (RIP) Assay
28. RIP-seq in Epigenetic Analyses
29. Chromatin Isolation by RNA Purification (ChIRP) and Its Applications
Section X: Additional Epigenetics Methods
30. Single-Cell Epigenomics: Methods and Translation
31. CRISPR/Cas9 Technologies in Epigenetics Research
32. Recent Advances in Computational-Based Approaches in Epigenetics Studies
33. Epigenome-Wide Association Study (EWAS) Methods and Applications
34. Methods in Epigenetics-Based Systems Biology and Their Applications
35. Forensic Epigenetics Methods and Applications
Section XI: Future Directions in Epigenetics Methodology
36. Advances in Epigenetic Techniques to Study Development and Diseases
1. An Overview of Epigenetics Methods
Section II: DNA Methylation Methods: Gene- or Locus-specific Methylation Analyses
2. Principles of Bisulfite Conversion of DNA and Methylation-specific PCR (MSP) in Biological Research
3. Analysis of DNA Methylation Using Pyrosequencing
4. Matrix-assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF-MS) for Analysis of DNA Methylation
5. Methylation-Specific Droplet Digital PCR (MS-ddPCR) for Detection and Absolute Quantification of Rare Methylated Alleles
Section III: DNA Methylation Methods: Global DNA Methylation and Methylomic Analyses
6. ELISA Analyses of Global Methylation Levels
7. Bisulfite PCR of Repetitive Genomic Sequences
8. The Illumina Infinium Methylation Assay for Genome-wide Methylation Analyses
9. Analysis of Genome-wide Methylation Using Reduced Representation Bisulfite Sequencing (RRBS) Technology
10. Methylated DNA Immunoprecipitation Sequencing (MeDIP-seq): Principles and Applications
11. Multiplex Analyses Using Methylation Sensitive Restriction Enzyme qPCR
12. Advances in Whole Genome Methylomic Sequencing
Section IV: DNA Methylation Methods: Additional Technologies
13. Analyzing DNA Methyltransferase Enzymatic Activity: Basic Methods and Applications
14. Technologies and Applications for the Assessment of 5-Hydroxymethylcytosine
Section V: Histone Modification Methods
15. The Chromatin Immunoprecipitation (ChIP) Assay and ChIP-qPCR
16. Deep Sequencing for Histone Modifications Employing ChIP-seq Analyses
17. ChIP in Series: Sequential ChIP
18. ChIP-exo: A Method to Study Chromatin Structure and Organization at Near-Nucleotide Resolution
19. Monitoring Genome-wide Chromatin Accessibility by Formaldehyde-assisted Isolation of Regulatory Elements Sequencing (FAIRE-seq)
20. ATAC-seq Method Applied to Embryonic Germ Cells and Neural Stem Cells from Mouse: Practical Tips and Modifications
21. Combinatorial ChIP and Bisulfite Sequencing (BisChIP-seq)
Section VI: Epigenetic Methods for Evaluating Chromatin Higher Order
22. 3D DNA FISH for Analyses of Chromatin-Nuclear Architecture
23. Capture Hi-C: Characterization of Chromatin Contacts
Section VII: Evaluation of Chromosomal Proteins
24. Assessment of the Epigenetic Nature of Chromosomal Proteins During DNA Replication and Mitosis Section VIII: Enzyme Analyses of Histone Methylation
25. Assessment of Histone-Modifying Enzymes Involved in Epigenetic Gene Regulation
Section IX: Assessment of Non-Coding RNA as an Epigenetic Modulator
26. Detection and Quantification of MicroRNAs (miRNAs) and High Throughput miRNAs Profiling
27. Binding of Long Noncoding RNAs (lncRNAs) to RNA Binding Proteins Using the RNA Immunoprecipitation (RIP) Assay
28. RIP-seq in Epigenetic Analyses
29. Chromatin Isolation by RNA Purification (ChIRP) and Its Applications
Section X: Additional Epigenetics Methods
30. Single-Cell Epigenomics: Methods and Translation
31. CRISPR/Cas9 Technologies in Epigenetics Research
32. Recent Advances in Computational-Based Approaches in Epigenetics Studies
33. Epigenome-Wide Association Study (EWAS) Methods and Applications
34. Methods in Epigenetics-Based Systems Biology and Their Applications
35. Forensic Epigenetics Methods and Applications
Section XI: Future Directions in Epigenetics Methodology
36. Advances in Epigenetic Techniques to Study Development and Diseases
- Edition: 2
- Published: August 31, 2025
- No. of pages (Paperback): 750
- Imprint: Academic Press
- Language: English
- Paperback ISBN: 9780443267598
- eBook ISBN: 9780443267604
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Trygve O. Tollefsbol
Dr. Tollefsbol is a Distinguished Professor of Biology and a Senior Scientist in the O’Neal Comprehensive Cancer Center, Integrative Center for Aging Research, Nutrition Obesity Research Center, University Wide Microbiome Center, and the Comprehensive Diabetes Center at the University of Alabama at Birmingham (UAB). He is Director of the UAB Cell Senescence Culture Facility which he established in 1999. Dr. Tollefsbol trained as a Postdoctoral Fellow and Assistant Research Professor with members of the National Academy of Science at Duke University and the University of North Carolina. He earned doctorates in molecular biology and osteopathic medicine from the University of North Texas Health Sciences Center and his bachelor’s degree in Biology from the University of Houston. He has received prior funding from the NCI, NHLBI, NIMH and other federal institutes as well as the Glenn Foundation for Medical Research, Susan G. Komen for the Cure, the American Federation for Aging Research (AFAR), and the American Institute for Cancer Research (AICR) among many other sources.
Affiliations and expertise
Distinguished Professor of Biology, University of Alabama at Birmingham, AL, USA