Enzyme Engineering and Evolution: Specific Enzyme Applications
- 1st Edition, Volume 644 - September 15, 2020
- Latest edition
- Editor: Dan S. Tawfik
- Language: English
Methods in Enzymology, Volume 644, the latest release in this ongoing serial, continues the legacy of this premier serial with quality chapters authored by leaders in the field.… Read more
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Description
Description
Key features
Key features
- Provides the authority and expertise of leading contributors from an international board of authors
- Presents the latest release in the Methods in Enzymology series
Readership
Readership
Table of contents
Table of contents
1. Engineering lytic polysaccharide monooxygenases (LPMOs)
Zarah Forsberg, Anton A. Stepnov, Guro Kruge Nærdal, Geir Klinkenberg and Vincent G. H. Eijsink
2. Strategies for the expression and characterization of artificial myoglobin-based carbene transferases
Daniela M. Carminati, Eric J. Moore and Rudi Fasan
3. Methods for the detection and analysis of dioxygenase catalyzed dihydroxylation in mutant derived libraries
Julian L. Wissner, Wendy Escobedo-Hinojosa, Peter M. Heinemann, Andreas Hunold and Bernhard Hauer
4. Enhancing promiscuous chemistries of a Schiff-base forming enzyme by divergent evolution
Duncan S. Macdonald and Donald Hilvert
5. Glutamine-walking: Creating reactive substrates for transglutaminase-mediated protein labeling
Lukas Deweid, Adem H. Parisien, Kiana Lafontaine, Léa N.C. Rochet, Harald Kolmar and Joelle N. Pelletier
6. Engineering aldolases for asymmetric synthesis
Mikael Widersten
7. Ultra-high throughput screening for novel protease specificities
Eirini Rousounelou, Steven Schmitt, Luzius Pestalozzi, Martin Held, Tania M. Roberts and Sven Panke
8. Directed evolution of transcriptional switches using dual-selector systems
Yuki Kimura and Daisuke Umeno
9. Methods for competitive enrichment and evaluation of superior DNA ligases
Janine K. Sharma, Luke J. Stevenson, Katherine J. Robins, Adele K. Williamson, Wayne M. Patrick and David F. Ackerley
10. Directed evolution of custom polymerases using droplet microfluidics
Derek Vallejo, Ali Nikoomanzar and John C. Chaput
11. Optically guided mass spectrometry to screen microbial colonies for directed enzyme evolution
Pu Xue, Tong Si and Huimin Zhao
12. Split enzymes: Design principles and strategy
Shion A. Lim and James A. Wells
Product details
Product details
- Edition: 1
- Latest edition
- Volume: 644
- Published: September 16, 2020
- Language: English
About the editor
About the editor
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