
Enzymatic Plastic Degradation
- 1st Edition, Volume 648 - February 10, 2021
- Imprint: Academic Press
- Editors: Gert Weber, Uwe T. Bornscheuer, Ren Wei
- Language: English
- Hardback ISBN:9 7 8 - 0 - 1 2 - 8 2 2 0 1 2 - 2
- eBook ISBN:9 7 8 - 0 - 1 2 - 8 2 2 0 1 3 - 9
Enzymatic Plastic Degradation, Volume 648 in the Methods in Enzymology series, continues the legacy of this premier serial with chapters authored by leaders in the field. Chapte… Read more

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Request a sales quoteEnzymatic Plastic Degradation, Volume 648 in the Methods in Enzymology series, continues the legacy of this premier serial with chapters authored by leaders in the field. Chapters in this latest release include Evaluating plastic pollution and environmental degradation, Assessment methods for microplastic pollution in the oceans and fresh water, Exploring microbial consortia from various environments for plastic degradation, Characterization of filamentous fungi for attack on synthetic polymers via biological Fenton chemistry, Synthesis of radioactive-labeled nanoplastics for assaying the environmental (microbial) PS degradation, Exploring metagenome for plastic degrading enzymes, Cutinases from thermophilic bacteria (actinomycetes): from identification to functional and structural characterization, and much more.
- Provides the authority and expertise of leading contributors from an international board of authors
- Presents the latest release in the Methods in Enzymology series
- Covers the latest research and technologies in enzymatic plastic degradation
Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists
- Cover image
- Title page
- Table of Contents
- Copyright
- Contributors
- Preface
- Chapter One: Current status and future development of plastics: Solutions for a circular economy and limitations of environmental degradation
- Abstract
- 1: Introduction
- 2: Definitions
- 3: Biodegradation and biodegradability—Explanations and limits
- 4: Current plastic market and production
- 5: Plastic recycling
- 6: Plastic waste and disposal
- 7: Mismanagement of plastic disposal and recycling—Plastic littering
- 8: Political actions against plastic littering
- 9: Conclusion and future development
- 10: Methods
- Chapter Two: Methods for microplastic sampling and analysis in the seawater and fresh water environment
- Abstract
- 1: Introduction
- 2: Water sample collection methods
- 3: Sample pretreatment before identification
- 4: Sample identification
- 5: Data reporting
- 6: Blank and contamination control
- 7: Summary
- Acknowledgments
- Chapter Three: Exploring microbial consortia from various environments for plastic degradation
- Abstract
- 1: Introduction
- 2: Enrichment and cultivation of aerobic plastic-degrading consortia
- 3: Enrichment and cultivation of anaerobic plastic-degrading consortia
- 4: Future perspectives
- Chapter Four: Cultivation of filamentous fungi for attack on synthetic polymers via biological Fenton chemistry
- Abstract
- 1: Introduction
- 2: Rationale for the cultivation of fungi for Fenton chemistry-dependent attack on synthetic polymers
- 3: Materials, equipment and reagents
- 4: Protocols
- 5: Safety considerations
- 6: Applicable analytical methods to detect fungal effects on polymers
- 7: Summary
- Acknowledgments
- Chapter Five: Characterization of biodegradation of plastics in insect larvae
- Abstract
- 1: Introduction
- 2: Biodegradation of plastics in Tenebrio molitor
- 3: Analytical methods for plastic biodegradation
- 4: Protocols for the characterization of plastic degradation by T. molitor larvae
- 5: Results and analysis
- 6: Summary
- Acknowledgments
- Chapter Six: Quantification of polystyrene plastics degradation using 14C isotope tracer technique
- Abstract
- 1: Introduction
- 2: Protocols
- 3: Summary and outlook
- 4: Safety notes
- Acknowledgments
- Chapter Seven: Exploring the global metagenome for plastic-degrading enzymes
- Abstract
- 1: Introduction
- 2: Materials
- 3: Methods
- 4: Notes
- 5: Outlook and summary
- Chapter Eight: Cutinases from thermophilic bacteria (actinomycetes): From identification to functional and structural characterization
- Abstract
- 1: Introduction
- 2: Identification of thermophilic cutinases
- 3: Structural and thermodynamic analysis of PET-hydrolyzing cutinases
- 4: Protocols
- 5: Summary
- Acknowledgments
- Chapter Nine: Ideonella sakaiensis, PETase, and MHETase: From identification of microbial PET degradation to enzyme characterization
- Abstract
- 1: Introduction
- 2: Screening microorganisms that degrade PET and isolation of microbial consortium no. 46
- 3: Isolation of I. sakaiensis 201-F6 from microbial consortium no. 46
- 4: Detection of microbial PET degradation
- 5: Characterization of PETase and MHETase
- 6: Summary
- Chapter Ten: GRAPE, a greedy accumulated strategy for computational protein engineering
- Abstract
- 1: Introduction
- 2: Materials
- 3: Methods
- 4: Notes
- 5: Summary
- Chapter Eleven: Mechanistic investigation of enzymatic degradation of polyethylene terephthalate by nuclear magnetic resonance
- Abstract
- 1: Introduction
- 2: 1H solution NMR analysis to quantify PET chain scissions
- 3: Solid-state NMR analysis to determine PET chain conformation and dynamics
- 4: Summary
- Chapter Twelve: Fluorimetric high-throughput screening method for polyester hydrolase activity using polyethylene terephthalate nanoparticles
- Abstract
- 1: Introduction
- 2: PET nanoparticles: Generation, application, and characterization
- 3: Fluorimetric high-throughput screening assay
- 4: Materials, equipment, and reagents
- 5: Protocols
- Acknowledgments
- Chapter Thirteen: Anchor peptides promote degradation of mixed plastics for recycling
- Abstract
- 1: Introduction
- 2: Engineering the adhesion peptide binding modules for enhanced polymer absorption and enzymatic degradation
- 3: Equipment
- 4: Materials
- 5: Protocol
- 6: Summary and outlook
- Acknowledgments
- Chapter Fourteen: Tuning of adsorption of enzymes to polymer
- Abstract
- 1: Introduction
- 2: Expression and purification of polymer degrading hydrolases
- 3: Surface engineering by site-directed mutagenesis
- 4: Fusion of hydrophobic binding domains
- 5: Enzyme truncation and mutagenesis of the metal-binding site
- 6: Summary
- Chapter Fifteen: Characterization of the enzymatic degradation of polyurethanes
- Abstract
- 1: Introduction
- 2: Materials, equipment and reagents
- 3: Protocol
- 4: Safety considerations and standards
- 5: Analysis and statistics
- 6: Pros and cons
- 7: Alternative methods/procedures
- 8: Troubleshooting and optimization
- 9: Summary
- Acknowledgments
- Chapter Sixteen: Structural analysis of PET-degrading enzymes PETase and MHETase from Ideonella sakaiensis
- Abstract
- 1: Introduction
- 2: Methods background
- 3: Expression and purification
- 4: Crystallization
- 5: Modeling of a PETase-BHET complex
- 6: Summary
- Acknowledgments
- Chapter Seventeen: Structural and functional characterization of nylon hydrolases
- Abstract
- 1: Introduction
- 2: Screening of microorganisms and enzymes degrading nylon-related compounds
- 3: Preparation of oligomeric and polymeric substrates for enzyme assays
- 4: Enzymatic hydrolysis of nylons and related substrates
- 5: Structural analysis of 6-aminohexanoate dimer hydrolase NylB
- 6: Protocol
- 7: Summary
- Acknowledgments
- Chapter Eighteen: Upcycling of hydrolyzed PET by microbial conversion to a fatty acid derivative
- Abstract
- 1: Introduction
- 2: Protocols
- 3: Conclusion
- Chapter Nineteen: Screening and cultivating microbial strains able to grow on building blocks of polyurethane
- Abstract
- 1: Introduction
- 2: Methods
- 3: Outlook
- Acknowledgment
- Chapter Twenty: Engineering microalgae as a whole cell catalyst for PET degradation
- Abstract
- 1: Introduction
- 2: Microalgae as phototrophic cell factories for recombinant protein production
- 3: Introducing the PETase gene into the genome of a diatom
- 4: Protocol
- 5: Analysis of enzymatic plastic degradation
- 6: Summary
- Acknowledgments
- Chapter Twenty-One: Yeast cell surface display of bacterial PET hydrolase as a sustainable biocatalyst for the degradation of polyethylene terephthalate
- Abstract
- 1: Introduction
- 2: Protocols
- Acknowledgment
- Edition: 1
- Volume: 648
- Published: February 10, 2021
- Imprint: Academic Press
- No. of pages: 500
- Language: English
- Hardback ISBN: 9780128220122
- eBook ISBN: 9780128220139
GW
Gert Weber
Gert Weber is at Helmholtz-Zentrum Berlin, Germany
Affiliations and expertise
Helmholtz-Zentrum Berlin, GermanyUB
Uwe T. Bornscheuer
Prof. Uwe Bornscheuer from Greifswald University is a leading expert in enzymatic lipid modification as documented in numerous peer-reviewed publications, reviews as well as patents. He has received several scientific awards including the Chevreul Medal (French Lipid Society, 2012), the Normann Medal (DGF, Germany, 2014) and the Stephen S. Chang Award (AOCS, 2015).
Affiliations and expertise
Greifswald University, GermanyRW
Ren Wei
Ren Wei is at Universität Greifswald, Germany
Affiliations and expertise
Universität Greifswald, GermanyRead Enzymatic Plastic Degradation on ScienceDirect