Engineering Biology for Microbial Biosynthesis of Plant-Derived Bioactive Compounds

1st Edition - March 1, 2024
Editors: Yongjun Wei, Xiao-Jun Ji, Mingfeng Cao
Language: English
Paperback ISBN:
9 7 8 - 0 - 4 4 3 - 1 5 5 5 8 - 1
eBook ISBN:
9 7 8 - 0 - 4 4 3 - 1 5 5 5 9 - 8

Engineering Biology for Microbial Biosynthesis of Plant-Derived Bioactive Compounds will be of great interest to undergraduate and graduate students, scientists, researche… Read more

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Engineering Biology for Microbial Biosynthesis of Plant-Derived Bioactive Compounds will be of great interest to undergraduate and graduate students, scientists, researchers, and engineers who work on the microbial production of plant-derived bioactive compounds. As many plant-derived bioactive compounds are the foundation of effective drugs to cure diseases, it's important to understand the limits of their applications in drugs and other industries. With the development of synthetic biology and green biomanufacturing, the biosynthesis of complex bioactive compounds is possible. However, the engineering fundamentals for microbial biosynthetic of plant-derived bioactive compounds is vast.

This book summarizes the engineering fundamental biotechnologies for microbial production of plant-derived bioactive compounds, allowing the content to be easily accessed. Coverage includes summaries of key achievements, identification of challenges, current approaches, tips and tricks, and helpful insights into critical bottlenecks.

Cover image
Title page
Table of Contents
Copyright
List of contributors
Preface
Chapter 1. Mining bioparts for the biosynthesis of plant-derived bioactive compounds via omics and bioinformatics technologies
Abstract
1.1 Status of omics and bioinformatics in mining bioparts of plant-derived bioactive compounds
1.2 The biosynthetic pathways and key enzymes of plant-derived bioactive compounds
1.3 The bioparts of plant-derived bioactive compounds
1.4 Bioinformatics integrates omics data to efficiently mine bioparts
1.5 Conclusions and future prospects
References
Chapter 2. DNA assembly techniques for the reconstitution of plant natural product biosynthetic pathways in Saccharomyces cerevisiae
Abstract
2.1 Introduction
2.2 Techniques for the assembly of multigene biosynthetic pathways in plasmids
2.3 Techniques for the assembly of multigene biosynthetic pathways in the yeast genome
2.4 Summary and perspectives
Acknowledgments
Declaration of interests
References
Chapter 3. The application of gene-editing technologies in the biosynthesis of plant-derived bioactive compounds
Abstract
3.1 Introduction
3.2 Brief principle of CRISPR-Cas9 system
3.3 Metabolic engineering of plant-derived bioactive compounds in fungi by CRISPR-Cas9 technology
3.4 Metabolic engineering in bacteria by CRISPR-Cas9 technology
Acknowledgments
Declaration of interest
References
Chapter 4. Pathway engineering of plant-derived bioactive compounds in microbes
Abstract
4.1 Introduction
4.2 Discovery and pathway elucidation of natural products in plants
4.3 Reconstitution of plant-derived bioactive compound pathways in microbes
4.4 Novel methods
4.5 Applications and future horizons of plant-derived bioactive compounds
4.6 Perspectives
Acknowledgments
CRediT authorship contribution statement
Declaration of interests
References
Chapter 5. Construction and application of a genome-scale metabolic network model for plants
Abstract
5.1 Research status of plant metabolic network model
5.2 The construction of plant metabolic network model
5.3 Application of plant metabolic network model
5.4 Prospects
Acknowledgment
Declaration of interest
References
Chapter 6. Metabolic design–build–test–learn cycle used for the biosynthesis of plant-derived bioactive compounds
Abstract
6.1 Introduction
6.2 Design
6.3 Build
6.4 Test
6.5 Learn
6.6 Perspectives
References
Chapter 7. Microbial cell factories for the synthesis of plant-derived bioactive compounds: metabolic flux dynamic regulation
Abstract
7.1 Fine-tuning gene expression
7.2 Cofactor balance
7.3 Coculture system
7.4 Future perspective
Acknowledgment
Declaration of interest
References
Chapter 8. Yeast cell factories for the biosynthesis of plant-derived bioactive terpenoids
Abstract
8.1 Introduction
8.2 Monoterpenes
8.3 Sesquiterpenes
8.4 Diterpenoids
8.5 Triterpenoids
8.6 Tetraterpenes
Acknowledgment
Declaration of interest
References
Chapter 9. Nonconventional yeast cell factories for the biosynthesis of plant-derived bioactive terpenoids
Abstract
9.1 Introduction
9.2 Biosynthetic pathway of terpenoids
9.3 Typical terpenoid products
9.4 Metabolic engineering strategy for optimizing terpenoids synthesis
9.5 Conclusion and prospect
Acknowledgments
Authorship contribution statement
Declaration of competing interests
References
Chapter 10. Nonconventional yeast cell factories for the biosynthesis of plant-derived bioactive flavonoids
Abstract
10.1 Introduction
10.2 The biosynthetic pathway of flavonoids
10.3 Nonconventional yeasts for the biosynthesis of flavonoids
10.4 Metabolic engineering strategies for optimizing flavonoids synthesis
10.5 Conclusion and prospects
Acknowledgments
Authorship contribution statement
Declaration of competing interests
References
Chapter 11. Non-yeast fungal cell factories for the biosynthesis of plant-derived bioactive compounds
Abstract
11.1 Common industrial mold-based fungal cell factories
11.2 Plant-associated endophytes
11.3 Mushroom-based cell factories
11.4 Conclusions and future perspectives
Acknowledgment
Declaration of interest
References
Chapter 12. Fine-tuning and dynamic control of microbial cell factories for the biosynthesis of plant-derived bioactive compounds
Abstract
12.1 Introduction
12.2 Fine-tune strategies for reconstruction of metabolic flux balance
12.3 Dynamic control strategies and their applications in the biosynthesis of plant-derived bioactive compounds
12.4 Future perspectives
References
Chapter 13. Biofoundries for plant-derived bioactive compounds
Abstract
13.1 Biofoundries
13.2 Laboratory automation
13.3 Cloud laboratory
13.4 The robot scientist
13.5 The successful biofoundries
13.6 Perspective
Acknowledgment
Declaration of interest
References
Chapter 14. Engineering biology fundamental for plant-derived bioactive compounds: challenges and prospects
Abstract
14.1 Introduction
14.2 Challenges and strategies at the “point” level
14.3 Challenges and strategies at the “line” level
14.4 Challenges and strategies at the “plane” level
14.5 Challenges and strategies at the “system” level
References
Chapter 15. Reprogramming microbial cell factories to overproduce plant natural products through directed genome evolution
Abstract
15.1 Terpenoids
15.2 Phenylpropanoids
15.3 Conclusion and perspective
Acknowledgment
Declaration of interest
References
Index

Yongjun Wei

Dr. Yongjun Wei, PhD, completed his bachelor’s degree in biology at Shaanxi Normal University, China, before pursuing his PhD in microbiology from the Chinese Academy of Sciences (CAS) in 2014. Following his doctoral studies, he conducted a postdoctoral fellowship at the Chalmers University of Technology from 2014 to 2016. After that, he worked as an assistant researcher at CAS for a year before joining Zhengzhou University’s faculty in 2018. Currently he serves as an associate professor at Zhengzhou University, Dr. Wei has published over 80 peer-reviewed papers and holds more than 20 patents. Furthermore, he serves as an editorial board member or youth editor for over 10 different journals. His research primarily focuses on microbial production of plant bioactive natural products and the utilization of microorganisms for disease treatment and pollution eradication.

Affiliations and expertise

School of Pharmaceutical Sciences, Laboratory of Synthetic Biology, Food Laboratory of Zhongyuan, Zhengzhou University, Zhengzhou, China

Xiao-Jun Ji

Dr. Xiao-Jun Ji, PhD, is currently a professor of bioengineering and biochemical engineering at Nanjing Tech University (NanjingTech), China. He received his BSc and PhD degrees from NanjingTech in 2005 and 2009 and conducted the visiting research in the Systems and Synthetic Biology lab headed by Prof. Jens Nielsen at Chalmers, Sweden during 2016 and 2017. His recent research focuses on biomanufacturing pharmaceutical and nutritional chemicals using the nonconventional yeasts through the metabolic engineering and the emerging synthetic biology tools. He has many awards such as the Fok Ying-Tung Foundation Young Scholars Award (2014), the Hou De-Bang Chemical Youth Award of CIESC (2016), the National Technological Invention Award of China (2018), the Excellent Young Scholars of NSFC (2019), and the Newton Advanced Fellowships of the Royal Society (2020).

Affiliations and expertise

NanjingTech University, National Engineering Research Centre for Biotechnology , Nanjing, China

Mingfeng Cao

Dr. Mingfeng Cao, PhD, is the Minjiang distinguished professor in the Department of Chemical and Biochemical Engineering, the director of the Institute of Bioengineering, and an honorary research fellow of the Tan Kah Kee Innovation Laboratory at the Xiamen Laboratory, China. He was awarded his PhD from the Nankai University in 2011, and between 2011 and 2013 worked as a research associate at the Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences. He completed postdoctoral studies at Iowa State University, USA and was a research scientist at the University of Illinois from 2018 to 2021. His research focuses on the development of synthetic biology and metabolic engineering strategies to establish microbial cell factories for value-added and green chemical production. Dr. Cao has published over 60 journal articles and holds 10 patents. He also serves as the editor-in-chief of Biotechnology Reports and the associate editor for journals such as Microbial Cell Factories and Biotechnology for Biofuels and Bioproducts.

Affiliations and expertise

Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Key Laboratory for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen, China