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Electrophoresis

Theory, Methods, and Applications

1st Edition - January 1, 1967

Editor: Milan Bier

eBook ISBN:

9 7 8 - 1 - 4 8 3 2 - 7 4 2 9 - 4

Electrophoresis: Theory, Methods, and Applications, Volume II focuses on the contributions of electrophoresis in the advancement of knowledge on proteins, as well as in the fields… Read more

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Electrophoresis: Theory, Methods, and Applications, Volume II focuses on the contributions of electrophoresis in the advancement of knowledge on proteins, as well as in the fields of biochemistry, physiology, and medicine. The selection first offers information on the interpretation of electrophoretic mobilities, including theories for other models, electrophoresis of polyelectrolytes, and theory for a rigid spherical particle. The text then takes a look at primary protein structures and nomenclature and identification of the normal human serum proteins. Discussions focus on principles of nomenclature of the serum constituents; methods of identification of an isolated antigen; principal methods used to study serum proteins; separation of mixtures of peptides and amino acids by high-voltage electrophoresis; and methods for determining the primary structure of proteins. The publication elaborates on inheritance of protein variation in human serum and antibodies and myeloma proteins. Topics include products of enzymic digestion, products of reduction, naturally occurring fragments, genetic variation, and variations detected in human serum proteins. The manuscript then examines lymph and cerebrospinal fluid, electrophoresis of gastrointestinal secretions, and high resolution techniques. The selection is a dependable source of data for readers interested in electrophoresis.

List of Contributors

Preface

Contents of Volume I

Introduction

1. The Interpretation of Electrophoretic Mobilities

I. Introduction

II. General Considerations

III. Theory for a Rigid Spherical Particle

IV. Theories for Other Models

V. Electrophoresis of Polyelectrolytes

References

2. Primary Protein Structures

I. General Conclusions from Present Knowledge of Primary Protein Structure

II. Methods for Determining the Primary Structure of Proteins

III. Separation of Mixtures of Peptides and Amino Acids by High-Voltage Electrophoresis

References

3. Nomenclature and Identification of the Normal Human Serum Proteins

I. Introduction

II. Principal Methods Used to Study Serum Proteins

III. Methods of Identification of an Isolated Antigen

IV. Principles of Nomenclature of the Serum Constituents

V. The Immunoglobulins

VI. γ- and β2-Proteins Other Than Immunoglobulins

VII. The β1-Globulins

VIII. The α2-Globulins

IX. The α1-Globulins

X. Serum Albumin

XI. The α0-Globulins

XII. The Prealbumins (ρ-Proteins)

XIII. The Lipoproteins

XIV. The Serum Enzymes

References

4. Inheritance of Protein Variation in Human Serum

I. Genetic Variation

II. Variations Detected in Human Serum Proteins

References

5. Antibodies and Myeloma Proteins

I. Introduction

II. Whole Proteins

III. Products of Enzymic Digestion

IV. Products of Reduction

V. Naturally Occurring Fragments

VI. Concluding Remarks

References

6. Lymph and Cerebrospinal Fluid

I. Introduction

II. Lymph

III. Cerebrospinal Fluid

References

7. Electrophoresis of Gastrointestinal Secretions

I. Introduction

II. Saliva

III. Gastric Juice

IV. Pancreatic Juice

V. Duodenal and Jejunal Juice and Meconium

VI. Bile

References

8. High Resolution Techniques

I. Introduction

II. Analytical Methods

III. Preparative Procedures

IV. Photography of Gels

V. Difficulties and Limitations

References

9. Preparative Electrophoresis

I. Introduction

II. Electrophoresis in Homogeneous Media

III. Zone Electrophoresis on Supporting Media

IV. Continuous Zone Electrophoresis

References

10. Automatic Density Gradient Electrophoresis

I. Introduction

11. Principles and Methods

III. The Automatic Electrophoresis Apparatus

References

11. Electrophoresis in Semifluid Films

I. Introduction

II. Procedures and Control of Critical Factors

III. Applications

IV. Detection and Quantitation by Far Ultraviolet Photometry

V. The Spectrophor

References

Author Index

Subject Index