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Electrophoresis
Theory, Methods, and Applications
1st Edition - January 1, 1967
Editor: Milan Bier
eBook ISBN:9781483274294
9 7 8 - 1 - 4 8 3 2 - 7 4 2 9 - 4
Electrophoresis: Theory, Methods, and Applications, Volume II focuses on the contributions of electrophoresis in the advancement of knowledge on proteins, as well as in the fields… Read more
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Electrophoresis: Theory, Methods, and Applications, Volume II focuses on the contributions of electrophoresis in the advancement of knowledge on proteins, as well as in the fields of biochemistry, physiology, and medicine. The selection first offers information on the interpretation of electrophoretic mobilities, including theories for other models, electrophoresis of polyelectrolytes, and theory for a rigid spherical particle. The text then takes a look at primary protein structures and nomenclature and identification of the normal human serum proteins. Discussions focus on principles of nomenclature of the serum constituents; methods of identification of an isolated antigen; principal methods used to study serum proteins; separation of mixtures of peptides and amino acids by high-voltage electrophoresis; and methods for determining the primary structure of proteins. The publication elaborates on inheritance of protein variation in human serum and antibodies and myeloma proteins. Topics include products of enzymic digestion, products of reduction, naturally occurring fragments, genetic variation, and variations detected in human serum proteins. The manuscript then examines lymph and cerebrospinal fluid, electrophoresis of gastrointestinal secretions, and high resolution techniques. The selection is a dependable source of data for readers interested in electrophoresis.
List of Contributors
Preface
Contents of Volume I
Introduction
1. The Interpretation of Electrophoretic Mobilities
I. Introduction
II. General Considerations
III. Theory for a Rigid Spherical Particle
IV. Theories for Other Models
V. Electrophoresis of Polyelectrolytes
References
2. Primary Protein Structures
I. General Conclusions from Present Knowledge of Primary Protein Structure
II. Methods for Determining the Primary Structure of Proteins
III. Separation of Mixtures of Peptides and Amino Acids by High-Voltage Electrophoresis
References
3. Nomenclature and Identification of the Normal Human Serum Proteins
I. Introduction
II. Principal Methods Used to Study Serum Proteins
III. Methods of Identification of an Isolated Antigen
IV. Principles of Nomenclature of the Serum Constituents
V. The Immunoglobulins
VI. γ- and β2-Proteins Other Than Immunoglobulins
VII. The β1-Globulins
VIII. The α2-Globulins
IX. The α1-Globulins
X. Serum Albumin
XI. The α0-Globulins
XII. The Prealbumins (ρ-Proteins)
XIII. The Lipoproteins
XIV. The Serum Enzymes
References
4. Inheritance of Protein Variation in Human Serum
I. Genetic Variation
II. Variations Detected in Human Serum Proteins
References
5. Antibodies and Myeloma Proteins
I. Introduction
II. Whole Proteins
III. Products of Enzymic Digestion
IV. Products of Reduction
V. Naturally Occurring Fragments
VI. Concluding Remarks
References
6. Lymph and Cerebrospinal Fluid
I. Introduction
II. Lymph
III. Cerebrospinal Fluid
References
7. Electrophoresis of Gastrointestinal Secretions
I. Introduction
II. Saliva
III. Gastric Juice
IV. Pancreatic Juice
V. Duodenal and Jejunal Juice and Meconium
VI. Bile
References
8. High Resolution Techniques
I. Introduction
II. Analytical Methods
III. Preparative Procedures
IV. Photography of Gels
V. Difficulties and Limitations
References
9. Preparative Electrophoresis
I. Introduction
II. Electrophoresis in Homogeneous Media
III. Zone Electrophoresis on Supporting Media
IV. Continuous Zone Electrophoresis
References
10. Automatic Density Gradient Electrophoresis
I. Introduction
11. Principles and Methods
III. The Automatic Electrophoresis Apparatus
References
11. Electrophoresis in Semifluid Films
I. Introduction
II. Procedures and Control of Critical Factors
III. Applications
IV. Detection and Quantitation by Far Ultraviolet Photometry