Electron Paramagnetic Resonance Investigations of Biological Systems by Using Spin Labels, Spin Probes, and Intrinsic Metal Ions Part B
- 1st Edition, Volume 564 - October 7, 2015
- Imprint: Academic Press
- Editors: Peter Z Qin, Kurt Warncke
- Language: English
- Hardback ISBN:9 7 8 - 0 - 1 2 - 8 0 2 8 3 5 - 3
- eBook ISBN:9 7 8 - 0 - 1 2 - 8 0 2 8 4 7 - 6
Electron Paramagnetic Resonance Investigations of Biological Systems by Using Spin Labels, Spin Probes, and Intrinsic Metal Ions Part A & B, are the latest volumes in the Metho… Read more
Purchase options
Institutional subscription on ScienceDirect
Request a sales quoteElectron Paramagnetic Resonance Investigations of Biological Systems by Using Spin Labels, Spin Probes, and Intrinsic Metal Ions Part A & B, are the latest volumes in the Methods in Enzymology series, continuing the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods centered on the use of Electron Paramagnetic Resonance (EPR) techniques to study biological structure and function.
- Timely contribution that describes a rapidly changing field
- Leading researchers in the field
- Broad coverage: Instrumentation, basic theory, data analysis, and applications
Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists.
- Preface
- Section I: Spin Labeling Studies of Proteins
- Chapter One: Saturation Recovery EPR and Nitroxide Spin Labeling for Exploring Structure and Dynamics in Proteins
- Abstract
- 1 Introduction
- 2 Theoretical Background and the Measurement of T1e with SR
- 3 Instrumentation and Practical Considerations
- 4 Applications of Long-Pulse SR
- 5 Summary and Future Directions
- Chapter Two: High-Pressure EPR and Site-Directed Spin Labeling for Mapping Molecular Flexibility in Proteins
- Abstract
- 1 Introduction
- 2 Variable-Pressure CW EPR
- 3 Pressure-Resolved DEER
- 4 Example Applications and Perspectives
- 5 Summary and Future Directions
- Chapter Three: Exploring Structure, Dynamics, and Topology of Nitroxide Spin-Labeled Proteins Using Continuous-Wave Electron Paramagnetic Resonance Spectroscopy
- Abstract
- 1 Introduction
- 2 Site-Directed Spin Labeling
- 3 Analysis of EPR Spectra in Terms of R1 Nitroxide Motion
- 4 Measurement of R1 Solvent Accessibility With Power Saturation
- 5 CW-Based Interspin Distance Measurements
- 6 Determination of Secondary Structure and Features of the Tertiary Fold from R1 Mobility and Accessibility
- 7 Backbone Dynamics
- 8 Identifying Slow Conformational Exchange and Detecting Conformational Changes
- 9 Other Applications of CW EPR
- 10 Conclusion and Future Directions
- Chapter Four: Bifunctional Spin Labeling of Muscle Proteins: Accurate Rotational Dynamics, Orientation, and Distance by EPR
- Abstract
- 1 Introduction
- 2 Methods of BSL Labeling
- 3 Rotational Dynamics
- 4 Orientation
- 5 Distance
- 6 Discussion
- Chapter Five: EPR Distance Measurements in Deuterated Proteins
- Abstract
- 1 Introduction and Overview
- 2 Deuteration
- 3 The Pulse EPR Experiment
- 4 Data Analysis
- 5 Concluding Remarks
- Acknowledgments
- Chapter Six: Spin labeling and Double Electron-Electron Resonance (DEER) to Deconstruct Conformational Ensembles of HIV Protease
- Abstract
- 1 Perspective—Conformational Sampling of HIV-1 Protease
- 2 Site-Directed Spin-Labeling Electron Paramagnetic Resonance Spectroscopy
- 3 DEER Distance Profiles Reflect the Fractional Occupancies of HIV-1PR Conformational States
- 4 “DEERconstruct,” a Tool for Statistical Analysis of DEER Distance Profiles
- Chapter One: Saturation Recovery EPR and Nitroxide Spin Labeling for Exploring Structure and Dynamics in Proteins
- Section II: Spin Labeling Studies of Membrane and Membrane-Associated Proteins
- Chapter Seven: Ionizable Nitroxides for Studying Local Electrostatic Properties of Lipid Bilayers and Protein Systems by EPR
- Abstract
- 1 Introduction
- 2 EPR Characterization of pH-Sensitive Thiol-Specific Nitroxide Labels for Mapping Local Protein Electrostatics
- 3 pH-Sensitive Spin-Labeled Lipids for Measuring Surface Electrostatics of Lipid Bilayers by EPR
- 4 Conclusions and Outlook
- Acknowledgments
- Chapter Eight: Peptide–Membrane Interactions by Spin-Labeling EPR
- Abstract
- 1 Introduction
- 2 Peptide Labeling with EPR Active Probes and Preparation of Membrane Mimetic Systems
- 3 EPR Measurements of Membrane Peptide Binding
- 4 Analysis of Binding Isotherms
- 5 Topology of Membrane-Associated Peptides: Paramagnetic Relaxers Accessibility EPR Experiments
- 6 Detecting Membrane-Induced Aggregation and Agglomeration of Peptides
- 7 Conclusions and Outlook
- Acknowledgments
- Chapter Nine: Structural Characterization of Membrane-Curving Proteins: Site-Directed Spin Labeling, EPR, and Computational Refinement
- Abstract
- 1 Introduction
- 2 SDSL, EPR, and Computational Refinement Are a Powerful Combination for Studying Membrane-Curving Proteins Bound to Membranes of Defined Curvature
- 3 Sample Preparation Methodology for SDSL
- 4 EPR Measurements
- 5 Computational Refinement
- 6 Outlook
- Acknowledgment
- Chapter Ten: Determining the Secondary Structure of Membrane Proteins and Peptides Via Electron Spin Echo Envelope Modulation (ESEEM) Spectroscopy
- Abstract
- 1 Introduction
- 2 Integration of Membrane Peptides into Lipid Bilayer
- 3 ESEEM Spectroscopy on Model Peptides in a Lipid Bilayer
- 4 Development of ESEEM Secondary Structure Determination Approach
- 5 Summary and Future Direction
- Acknowledgments
- Chapter Eleven: Spin Labeling Studies of Transmembrane Signaling and Transport: Applications to Phototaxis, ABC Transporters and Symporters
- Abstract
- 1 Introduction
- 2 Applications to Membrane Proteins
- 3 Outlook
- Acknowledgments
- Chapter Twelve: Navigating Membrane Protein Structure, Dynamics, and Energy Landscapes Using Spin Labeling and EPR Spectroscopy
- Abstract
- 1 Introduction
- 2 An EPR Primer
- 3 Principles of DEER Spectroscopy to Uncover Conformational Dynamics
- 4 Practical Considerations in Sample Preparation
- 5 Perspective
- Acknowledgments
- Chapter Thirteen: Spin Labeling of Potassium Channels
- Abstract
- 1 Introduction
- 2 General Considerations for Spin Labeling Studies of Potassium Channels
- 3 Examples of Labeling Methods
- Chapter Seven: Ionizable Nitroxides for Studying Local Electrostatic Properties of Lipid Bilayers and Protein Systems by EPR
- Section III: Spin Labeling Studies of Nucleic Acids
- Chapter Fourteen: Advanced EPR Methods for Studying Conformational Dynamics of Nucleic Acids
- Abstract
- 1 Introduction
- 2 Spin Labels
- 3 Theory for Orientation-Selective PELDOR
- 4 Experimental Procedure for Multifrequency/Multifield PELDOR
- 5 Analysis of Multifrequency/Multifield PELDOR Data
- 6 Summary and Outlook
- Acknowledgments
- Chapter Fifteen: An Integrated Spin-Labeling/Computational-Modeling Approach for Mapping Global Structures of Nucleic Acids
- Abstract
- 1 Introduction
- 2 SDSL of Nucleic Acids Using a Nucleotide-Independent Nitroxide Probe
- 3 Measuring Inter-R5 Distances Using Double Electron–Electron Resonance Spectroscopy
- 4 Integration of DEER-Measured Distances with Computational Modeling
- 5 Conclusions
- Acknowledgment
- Chapter Fourteen: Advanced EPR Methods for Studying Conformational Dynamics of Nucleic Acids
- Section IV: EPR-NMR Methods and Applications
- Chapter Sixteen: Overhauser Dynamic Nuclear Polarization Studies on Local Water Dynamics
- Abstract
- 1 Introduction
- 2 Theory
- 3 Hardware
- 4 Data Acquisition
- 5 Data Analysis
- 6 Examples of ODNP
- 7 Summary
- Acknowledgments
- Chapter Seventeen: Practical Aspects of Paramagnetic Relaxation Enhancement in Biological Macromolecules
- Abstract
- 1 Introduction
- 2 Paramagnetic Labels for PRE Measurements
- 3 Measurement of the PRE
- 4 Using the PRE in Structure Determination
- 5 Using the PRE to Detect Transient Sparsely Populated States
- Acknowledgments
- Chapter Sixteen: Overhauser Dynamic Nuclear Polarization Studies on Local Water Dynamics
- Section V: In Vivo EPR Oxymetry and Imaging
- Chapter Eighteen: In Vivo pO2 Imaging of Tumors: Oxymetry with Very Low-Frequency Electron Paramagnetic Resonance
- Abstract
- 1 Introduction: The Importance of Imaging Molecular Oxygen in Cancer Therapy
- 2 Summary
- Acknowledgments
- Chapter Nineteen: Direct and Repeated Measurement of Heart and Brain Oxygenation Using In Vivo EPR Oximetry
- Abstract
- 1 Introduction
- 2 Principles of EPR Oximetry
- 3 Paramagnetic Oxygen-Sensitive Probes for pO2 Measurements by EPR
- 4 Application of EPR Oximetry in Ischemic Pathologies
- 5 Summary
- Acknowledgments
- Chapter Twenty: Free Radical Imaging Using In Vivo Dynamic Nuclear Polarization-MRI
- Abstract
- 1 Introduction
- 2 Principle of DNP
- 3 Apparatus
- 4 Spin-Probe Technique for DNP-MRI
- 5 Application of DNP-MRI to the Intrinsic Molecular Imaging
- 6 Conclusion
- Acknowledgments
- Chapter Eighteen: In Vivo pO2 Imaging of Tumors: Oxymetry with Very Low-Frequency Electron Paramagnetic Resonance
- Author Index
- Subject Index
- Edition: 1
- Volume: 564
- Published: October 7, 2015
- No. of pages (Hardback): 634
- No. of pages (eBook): 634
- Imprint: Academic Press
- Language: English
- Hardback ISBN: 9780128028353
- eBook ISBN: 9780128028476
PQ
Peter Z Qin
Affiliations and expertise
Department of Chemistry, University of Southern California, USAKW
Kurt Warncke
Affiliations and expertise
Department of Physics, Emory University, USARead Electron Paramagnetic Resonance Investigations of Biological Systems by Using Spin Labels, Spin Probes, and Intrinsic Metal Ions Part B on ScienceDirect