DNA Interactions with Drugs and Other Small Ligands
Single Molecule Approaches and Techniques
- 1st Edition - March 7, 2023
- Latest edition
- Author: Marcio Santos Rocha
- Language: English
DNA Interactions With Drugs and Other Small Ligands: Single Molecule Approaches and Techniques provides the reader with all the main information, a "state-of-the-art" of sorts… Read more
DNA Interactions With Drugs and Other Small Ligands: Single Molecule Approaches and Techniques provides the reader with all the main information, a "state-of-the-art" of sorts and an overall review of the field. There is no other book currently available that covers all these subjects together. On the contrary, the different subjects that are developed in this book are currently scattered in journal articles and other books.
- Presents a review of the fundamental knowledge, techniques and relevant information surrounding the field of DNA interactions with drugs and other ligands
- Provides a resource like no other book available
- Includes valuable information from the author who is a highly experienced researcher in the field
academic sector, Potential readers include researchers, teachers and students (graduate and undergraduate) from the areas of physics, chemistry, biology, as well as from derived areas (biochemistry, biophysics, medicine, pharmacy, and others.), interdisciplinary courses at graduate level, especially in optional courses
1 – Introduction
1.1 Field overview
1.2 Basic concepts and definitions
2 – Interactions in soft matter systems
2.1 Fundamental interactions in nature
2.2 Soft matter materials
2.3 Intermolecular forces
3 – Polymers and biopolymers
3.1 Basic concepts and definitions
3.2 Mechanics of polymer chains
3.3 Structure and mechanical response of nucleic acids
4 – DNA ligands
4.1 Types of ligands and interactions
4.2 Chemical equilibrium and binding isotherms
4.3 Kinetics of the interactions
5 - Experimental techniques
5.1 Single molecule techniques
5.1.1 Optical tweezers
5.1.2 Magnetic tweezers
5.1.3 Atomic force microscopy
5.1.4 Other single molecule techniques
5.2 Bulk techniques
5.2.1 Optical spectroscopies
5.2.2 Gel electrophoresis
5.2.3 Microcalorimetry
5.2.4 Other bulk techniques
6 – Single molecule force spectroscopy of DNA-ligand complexes
6.1 Stretching nucleic acids
6.2 Entropic and enthalpic regimes
6.3 Determining the mechanical and structural parameters from force-extension assays
6.4 Extracting physical chemistry from the mechanical parameters
1.1 Field overview
1.2 Basic concepts and definitions
2 – Interactions in soft matter systems
2.1 Fundamental interactions in nature
2.2 Soft matter materials
2.3 Intermolecular forces
3 – Polymers and biopolymers
3.1 Basic concepts and definitions
3.2 Mechanics of polymer chains
3.3 Structure and mechanical response of nucleic acids
4 – DNA ligands
4.1 Types of ligands and interactions
4.2 Chemical equilibrium and binding isotherms
4.3 Kinetics of the interactions
5 - Experimental techniques
5.1 Single molecule techniques
5.1.1 Optical tweezers
5.1.2 Magnetic tweezers
5.1.3 Atomic force microscopy
5.1.4 Other single molecule techniques
5.2 Bulk techniques
5.2.1 Optical spectroscopies
5.2.2 Gel electrophoresis
5.2.3 Microcalorimetry
5.2.4 Other bulk techniques
6 – Single molecule force spectroscopy of DNA-ligand complexes
6.1 Stretching nucleic acids
6.2 Entropic and enthalpic regimes
6.3 Determining the mechanical and structural parameters from force-extension assays
6.4 Extracting physical chemistry from the mechanical parameters
- Edition: 1
- Latest edition
- Published: March 7, 2023
- Language: English
MR
Marcio Santos Rocha
The author graduated in Physics from the Federal University of Ceará (2001), Master in Physics from the Federal University of Minas Gerais (2004) and PhD in Physics from the Federal University of Minas Gerais (2008). Since 2008 he has been a professor in the Physics Department at the Federal University of Viçosa (UFV) in Brazil. He has experience in the field of Experimental Physics, with an emphasis on "Biological Physics", "Optics Applied to Biological Systems" and "Optical Manipulation of Microsystems". He coordinates the Biological Physics Laboratory at UFV, implemented by the researcher at this institution. Website: http://www.lfb.ufv.br. In addition. he is currently the coordinator of the Graduate Program in Physics at UFV. He has published more than 50 research articles since 2004 in the fields of “DNA-ligand interactions” and “Optical trapping and manipulation of microsystems”.
Affiliations and expertise
Associate Professor, Laboratório de Física Biológica (Biological Physics Lab), Departamento de Física, Universidade Federal de Viçosa (UFV), BrazilRead DNA Interactions with Drugs and Other Small Ligands on ScienceDirect