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DNA Damage and Chromosomal Instability
- 1st Edition, Volume 182 - February 1, 2024
- Editors: Christian Zierhut, Lorenzo Galluzzi
- Language: English
- Hardback ISBN:9 7 8 - 0 - 4 4 3 - 1 8 8 9 8 - 5
- eBook ISBN:9 7 8 - 0 - 4 4 3 - 1 8 8 9 9 - 2
DNA Damage and Chromosomal Instability, Volume 182 in the Methods in Cell Biology series, presents interesting chapters on a variety of timely topics, including Induction of… Read more
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Request a sales quoteDNA Damage and Chromosomal Instability, Volume 182 in the Methods in Cell Biology series, presents interesting chapters on a variety of timely topics, including Induction of chromosome-specific micronuclei and chromothripsis by centromere inactivation, Generation of aneuploid cells and assessment of their ability to survive in presence of chemotherapeutic agents, Genetic reporters to detect and quantify homologous recombination in yeast, Mapping histone variant genomic distribution: exploiting SNAP- Tag labelling to follow the dynamics of incorporation of H3 variants, Enrichment of DNA replication intermediates by EdU pull down, Nascent DNA sequencing and its diverse applications in genome integrity research, and much more. Additional chapters cover Enrichment of DNA replication intermediates by EdU pull down, DNA curtains for studying phase separation mechanisms of DNA-organizing proteins, Visualizing the Dynamics of DNA Replication and Repair at the Single-Molecule Molecule Level, Cloning and Expansion of Repetitive DNA Sequences, Using an ImageJ-based script to detect replication stress and associated cell cycle exit from G2 phase by fluorescence microscopy Arne Lindqvist
- Cover image
- Title page
- Table of Contents
- Series Page
- Copyright
- Contributors
- DNA damage and chromosomal instability
- References
- Chapter 1 Induction of chromosome-specific micronuclei and chromothripsis by centromere inactivation
- Abstract
- 1 Introduction
- 2 Y centromere inactivation using a CENP-A gene replacement strategy
- 3 Induction of Y chromosome-specific micronuclei
- 4 Y chromosome fragmentation following micronucleus induction
- 5 Y chromosome rearrangements following micronucleus induction
- 6 Summary
- Acknowledgments
- References
- Chapter 2 Generation of aneuploid cells and assessment of their ability to survive in presence of chemotherapeutic agents
- Abstract
- 1 Introduction
- 2 Materials
- 3 Methods
- 4 Notes
- Acknowledgments
- References
- Chapter 3 Genetic reporters to detect and quantify homologous recombination in yeast
- Abstract
- 1 Introduction
- 2 Materials
- 3 Methods
- 4 Limitations of the assays
- References
- Chapter 4 Mapping histone variant genomic distribution: Exploiting SNAP-tag labeling to follow the dynamics of incorporation of H3 variants
- Abstract
- 1 Introduction
- 2 Materials
- 3 Methods
- 4 Notes
- Acknowledgments
- References
- Chapter 5 Nascent DNA sequencing and its diverse applications in genome integrity research
- Abstract
- 1 Introduction
- 2 Methods
- 3 Notes
- References
- Chapter 6 Enrichment of DNA replication intermediates by EdU pull down
- Abstract
- 1 Introduction
- 2 EdU labelling and genomic DNA preparation
- 3 Click reaction and purification conditions
- 4 Enrichment of replication intermediates
- 5 Materials and reagents
- 6 Concluding remarks
- Acknowledgments
- References
- Chapter 7 DNA curtains for studying phase separation mechanisms of DNA-organizing proteins
- Abstract
- 1 Introduction
- 2 Current methods to study phase separated droplets
- 3 DNA curtain assay to unmask molecular mechanisms of phase separation
- 4 Notes
- 5 Conclusion
- References
- Chapter 8 Visualizing the dynamics of DNA replication and repair at the single-molecule level
- Abstract
- 1 Background and motivation
- 2 Methods
- 3 Conclusions
- References
- Chapter 9 Cloning and expansion of repetitive DNA sequences
- Abstract
- 1 Introduction
- 2 Methods
- 3 Protocol
- 4 Concluding remarks
- 5 Notes
- Acknowledgments
- References
- Chapter 10 Using an ImageJ-based script to detect replication stress and associated cell cycle exit from G2 phase by fluorescence microscopy
- Abstract
- 1 Introduction
- 2 Cell culture
- 3 Treatments
- 4 Immunofluorescence
- 5 Image acquisition
- 6 Image analysis (see note 10)
- 7 Quantification
- 8 Data visualization
- 9 Notes
- 10 Materials
- References
- Chapter 11 An automated image analysis pipeline to quantify the coordination and overlap of transcription and replication activity in mammalian genomes
- Abstract
- 1 Introduction
- 2 Notes
- References
- Chapter 12 Monitoring Chk1 kinase activity dynamics in live single cell imaging assays
- Abstract
- 1 Introduction
- 2 Live cell imaging tools to study cell cycle and DNA damage signaling
- 3 Image acquisition protocols
- 4 Notes
- 5 Image analysis
- References
- Chapter 13 Visualizing DNA damage and repair using single molecule super resolution microscopy
- Abstract
- 1 Introduction
- 2 Methods
- 3 Notes
- Acknowledgments
- References
- Chapter 14 Quantitative analysis of nuclear deformations and DNA damage foci dynamics by live-cell imaging
- Abstract
- 1 Introduction
- 2 Materials
- 3 Methods
- 4 Notes
- Acknowledgments
- References
- Chapter 15 Native FISH: A low- and high-throughput assay to analyze the alternative lengthening of telomere (ALT) pathway
- Abstract
- 1 Introduction
- 2 Materials
- 3 Method
- 4 Notes
- Acknowledgments
- References
- Chapter 16 Assessment of DNA fibers to track replication dynamics
- Abstract
- 1 Introduction
- 2 Methodology
- 3 Concluding remarks
- References
- Chapter 17 A high-content flow cytometry and dual CRISPR-Cas9 based platform to quantify genetic interactions
- Abstract
- 1 Introduction
- 2 Method
- 3 Notes
- Acknowledgment
- References
- Chapter 18 Monitoring APOBEC3A protein levels in human cancer cells
- Abstract
- 1 Introduction
- 2 Generation of anti-APOBEC3A monoclonal antibodies
- 3 Protocols
- 4 Conclusions
- References
- No. of pages: 318
- Language: English
- Edition: 1
- Volume: 182
- Published: February 1, 2024
- Imprint: Academic Press
- Hardback ISBN: 9780443188985
- eBook ISBN: 9780443188992
LG
Lorenzo Galluzzi
Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome “La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology.
Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals:
OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience.
Affiliations and expertise
Assistant Professor of Cell Biology in Radiation Oncology, Department of Radiation Oncology, Weill Cornell Medical College, NY, USARead DNA Damage and Chromosomal Instability on ScienceDirect