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Cytokinesis

1st Edition - January 2, 2017

Editor: Arnaud Echard

Hardback ISBN:

9 7 8 - 0 - 1 2 - 8 0 9 6 7 3 - 4

eBook ISBN:

9 7 8 - 0 - 1 2 - 8 0 9 7 9 2 - 2

Cytokinesis, the latest volume in the Methods in Cell Biology series, looks at the latest advances in cytokinesis. Edited by leaders in the field, this volume presents proven,… Read more

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Cytokinesis, the latest volume in the Methods in Cell Biology series, looks at the latest advances in cytokinesis. Edited by leaders in the field, this volume presents proven, state-of-art techniques, along with relevant historical background and theory, to aid researchers in efficient design and effective implementation of experimental methodologies.

Series Editors
Preface
Chapter 1. Labeling membrane domains in dividing echinoderm eggs
- 1. General Care and Use of Sea Urchins
- 2. Plasma Membrane Labels
- 3. Intracellular Labeling
Chapter 2. Dynamics of sphingomyelin- and cholesterol-enriched lipid domains during cytokinesis
- Introduction
- 1. Methods
- Conclusion
Chapter 3. Analysis of protein dynamics during cytokinesis in budding yeast
- Introduction
- 1. Five-Dimensional Live-Cell Imaging
- 2. Photoactivation and Photoconversion
- 3. Fluorescence Recovery After Photobleaching
Chapter 4. Imaging cytokinesis of Drosophila S2 cells
- Introduction
- 1. Methods
- Conclusion and Perspectives
Chapter 5. Studying cytokinesis in Drosophila epithelial tissues
- Introduction
- 1. Methods and Experimental Setup
- 2. Data Processing and Analysis
- 3. Markers and Genetic Tools to Study Epithelial Cytokinesis In Vivo
- Conclusions and Future Perspectives
Chapter 6. Visualization of cleavage furrow proteins in fixed dividing spermatocytes
- Introduction: Overview of Cytokinesis in Drosophila Spermatocytes
- 1. Fixation Protocols
- 2. Staining Protocols
Chapter 7. Monitoring complete and incomplete abscission in the germ line stem cell lineage of Drosophila ovaries
- Introduction
- 1. Methods
- Conclusion
Chapter 8. Incomplete cytokinesis/binucleation in mammals: The powerful system of hepatocytes
- Introduction
- 1. Experimental Procedures
- Conclusion
- Supplemendary Data
Chapter 9. Specific membrane dynamics during neural stem cell division
- Introduction
- 1. General Considerations
- 2. Isolation and Detection of Released Postabscission Midbodies
- 3. Live-Cell Imaging
- 4. High-Voltage Electron Microscopy
- Summary
Chapter 10. Understanding post-mitotic roles of the midbody during cell differentiation and polarization
- Introduction
- 1. Midbody and Epithelial Cell Polarity
- 2. Analysis of Midbody Uptake by Interphase Cells
- Summary
Chapter 11. Micromanipulation of daughter cells for the study of cytokinetic abscission
- Introduction
- 1. Adhesive Micropatterns
- 2. Laser Ablation of the Intercellular Bridge
Chapter 12. Measuring abscission spatiotemporal dynamics using quantitative high-resolution microscopy
- Introduction
- 1. Methods
Chapter 13. Analysis of cytokinesis by electron microscopy
- Introduction
- 1. Methods
- 2. Instrumentation and Materials
- 3. Discussion
Chapter 14. Studying cytokinesis and midbody remnants using correlative light/scanning EM
- Introduction
- 1. Experimental Procedures
- Conclusion
Chapter 15. Analysis of postcytokinetic roles of cytokinetic components in Caenorhabditis elegans
- Introduction
- 1. Generation of Transgenic Caenorhabditis elegans Expressing Fluorescent Cytokinetic Proteins
- 2. Creation of Conditional Mutations of Cytokinetic Genes in Caenorhabditis elegans Using the Somatic CRISPR-Cas9 Technique
- 3. Imaging of Cytokinesis and Postcytokinetic Events in Caenorhabditis elegans Larvae Using Spinning Disk Confocal Microscopy
- Conclusion
Chapter 16. Variations on a theme: Imaging cytokinetic and stable rings in situ using Caenorhabditis elegans
- Introduction
- 1. Methods
- Conclusions
- Supplementary Data
Chapter 17. Using fast-acting temperature-sensitive mutants to study cell division in Caenorhabditis elegans
- Introduction
- 1. Microscopy and Embryo Isolation
- 2. Using Fast-Acting Temperature Sensitive Mutant Embryos to Study Cytokinesis
- 3. Characterizing Temperature Sensitive Alleles
- 4. Choosing a Temperature Control Method
- Conclusions
Chapter 18. Cytokinesis from nanometers to micrometers and microseconds to minutes
- Introduction
- 1. Micropipette Aspiration
- 2. Fluorescence Correlation Spectroscopy
- 3. Fluorescence cross-correlation spectroscopy
- 4. Fluorescence Recovery After Photobleaching
- Conclusion
Chapter 19. An active contour ImageJ plugin to monitor daughter cell size in 3D during cytokinesis
- 1. Background
- 2. Methods
- Conclusion
- Appendix
Chapter 20. Nuclear displacement and fluorescence recovery after photobleaching (FRAP) assays to study division site placement and cytokinesis in fission yeast
- Introduction
- 1. Methods
- Conclusion
Chapter 21. Single-molecule measurements to study polymerization dynamics of FtsZ-FtsA copolymers
- Introduction: The Bacterial Cytoskeleton
- 1. Protein Biochemistry: Expression, Purification, and Fluorescent Labeling
- 2. Fluorescence Imaging of Protein Dynamics
- 3. Data Analysis
- Conclusion
Chapter 22. Preparation of centralspindlin as an active heterotetramer of kinesin and GTPase activating protein subunits for in vitro structural and functional assays
- Introduction
- 1. Purification of a Recombinant Caenorhabditis elegans Centralspindlin Complex Reconstituted in Bacterial Extract
- 2. Purification of Human Centralspindlin From HeLa Cells
- Conclusions
Chapter 23. In vitro reactivation of the cytokinetic contractile ring of fission yeast cells
- Introduction
- 1. Methods
- Conclusion
Chapter 24. Xenopus extract approaches to studying microtubule organization and signaling in cytokinesis
- Introduction
- 1. Actin-Intact Extract Preparation
- 2. Fluorescent Probes
- 3. Microtubule Organizing Centers and Microtubule Nucleation
- 4. Preparation of Glass Surfaces for Imaging
- 5. Protein Immunodepletion
- 6. Supported Lipid Bilayers
- 7. Droplet Microfluidics
Index
Volumes in Series