Current Topics in Cellular Regulation
- 1st Edition, Volume 30 - November 28, 1989
- Imprint: Academic Press
- Editors: Bernard L. Horecker, Earl R. Stadtman, P. Boon Chock
- Language: English
- Paperback ISBN:9 7 8 - 1 - 4 8 3 2 - 0 3 8 6 - 7
- eBook ISBN:9 7 8 - 1 - 4 8 3 2 - 1 7 2 9 - 1
Current Topics in Cellular Regulation: Volume 30 is a collection of papers that deals with eukaryotic DNA methylation, gene expression, regulation of nitrogenase activity, and the… Read more
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Request a sales quoteCurrent Topics in Cellular Regulation: Volume 30 is a collection of papers that deals with eukaryotic DNA methylation, gene expression, regulation of nitrogenase activity, and the recognition of DNA by Type II restriction enzymes. Other papers discuss the mitochondrial Krebs cycle, enzyme mechanisms, transducin, and calcium-dependent cellular processes. One paper explains that methylation and demethylation of the DNA are complicated active processes, involving many aspects of the genome. The paper also notes that within the gross changes in methylation patterns, specific CpG sites play important roles in the expression or shutdown of target genes. Another paper concludes that, mono-ADP ribosylation being a mechanism of action of the diphtheria toxin, ADP ribosylation plays a role in normal cell metabolism by regulating the activity of key enzymes. One paper explains the significance of restriction enzymes into the nature of DNA-protein interactions; as well as the enzyme's possible role in discriminating between alternative DNA sequences. Biochemists, cellular researchers, micro-biologists, bio-physicists, and academicians involved in the study of cellular biology will find the collection very useful.
Eukaryotic DNA Methylation and Gene Expression
I. Introduction
II. In Vitro Methylation by Mammalian DNA Methyltransferases
III. Methylation of the Mouse ß-Globin (Major) Gene
IV. DNA Sequence of the Mouse ß-Globin (Major) Gene
V. Genomic Sequencing: Direct Sequencing of Native, Uncloned DNA
VI. Concluding Remarks
References
Regulation of Nitrogenase Activity by Reversible ADP Ribosylation
I. Properties of Nitrogenase
II. Observations Leading to the Discovery of Covalent Modification of Dinitrogenase Reductase
III. Identification of ADP-Ribose as the Modifying Group
IV. Purification and Properties of Dinitrogenase Reductase-Activating Glycohydrolase
V. Reaction Catalyzed by Dinitrogenase Reductase-Activating Glycohydrolase
VI. Discovery, Purification, and Properties of Dinitrogenase Reductase ADP-Ribosyltransferase
VII. Role of ADP Ribosylation in the Physiology of Rhodospirillum rubrum
VIII. Role of Nucleotides in the Regulation of Switch-Off
IX. Role of Pyridine Nucleotides in the Regulation of Switch-Off
X. Demonstration That ADP Ribosylation Is Reversible in Vivo
XI. Regulation of the ADP Ribosylation Cycle
XII. Regulation of Dinitrogenase Reductase in Dark-Grown Cells
XIII. Effect of the Oxidation State of Dinitrogenase Reductase on Regulation in Vivo
XIV. Role of Divalent Metals in Regulation in Vivo
XV. Is ADP Ribosylation of Dinitrogenase Reductase a Fine-Tuning Device or an On-Off Switch?
XVI. Relationship to the Glutamine Synthetase Cascade
XVII. Genes for Dinitrogenase Reductase-Activating Glycohydrolase and Dinitrogenase Reductase ADP-Ribosyltransferase
XVIII. Properties of the ADP-Ribosylated Dinitrogenase Reductase
XIX. ADP Ribosylation in Other Organisms
XX. Other Roles for Dinitrogenase Reductase-Activating Glycohydrolase and Dinitrogenase Reductase ADP-Ribosyltransferase
XXI. Concluding Remarks
References
Recognition of DNA by Type II Restriction Enzymes
I. Outline of Restriction/Modification Systems
II. Molecular Mechanism of DNA Recognition
III. Genetic Analysis of Restriction/Modification Systems
IV. Characterization of Proteins
V. Catalytic Mechanisms of Restriction Enzymes
VI. DNA Sequence Specificity
VII. Location of Recognition Sites on DNA
VIII. Future Prospects
References
The Intracellular Site of Action of Insulin: The Mitochondrial Krebs Cycle
I. Introduction
II. Anabolic Processes Stimulated by Insulin
III. Hexokinase and Mitochondria
IV. Action of Insulin on the Gluconeogenic and Oxidative Pathways
V. Effects of Insulin on Energy Production and Utilization
VI. Receptor and the Glucose Transporter in Relation to the Hexokinase Mitochondrial Theory of Insulin Action: Summary and Predictions
References
Nonequilibrium Isotope Exchange Methods for Investigating Enzyme Mechanisms
I. Introduction
II. Theory
III. Measurement of Flux Ratios
IV. Applications
V. Discussion
References
Transducin: A Signaling Switch Regulated by Guanine Nucleotides
I. Introduction
II. cGMP Cascade in Visual Excitation
III. Subunits of Transducin
IV. Functional Domains of Tα
V. Generalized Coupling Mechanism Among Transduction Enzymes
VI. Concluding Remarks
References
Coordinate Interactions of Cyclic Nucleotide and Phospholipid Metabolizing Pathways in Calcium-Dependent Cellular Processes
I. Introduction
II. Lipids and cAMP
III. Lipids and cGMP
IV. Cyclic Nucleotide-Dependent Protein Kinases and Lipids
V. Lipids and Phosphodiesterases
VI. Summary and Conclusions
References
Index
- Edition: 1
- Volume: 30
- Published: November 28, 1989
- No. of pages (eBook): 260
- Imprint: Academic Press
- Language: English
- Paperback ISBN: 9781483203867
- eBook ISBN: 9781483217291
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