Crystallography of Protein Dynamics
- 1st Edition, Volume 688 - September 19, 2023
- Imprint: Academic Press
- Editor: Nozomi Ando
- Language: English
- Hardback ISBN:9 7 8 - 0 - 4 4 3 - 1 5 9 2 6 - 8
- eBook ISBN:9 7 8 - 0 - 4 4 3 - 1 5 9 2 7 - 5
Crystallography of Protein Dynamics, Volume 688, the latest release in this important series, highlights new advances in the field, with this new volume presenting intere… Read more
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Request a sales quoteCrystallography of Protein Dynamics, Volume 688, the latest release in this important series, highlights new advances in the field, with this new volume presenting interesting chapters written by an international board of authors.
- Provides the authority and expertise of leading contributors from an international board of authors
- Presents the latest release in the Methods in Enzymology series
- Includes the latest information on Crystallography of Protein Dynamics
Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists
- Cover image
- Title page
- Table of Contents
- Series Page
- Copyright
- Contributors
- Preface
- Chapter One: Introduction to diffuse scattering and data collection
- Abstract
- 1 Introduction
- 2 Theory
- 3 Samples for diffuse scattering
- 4 Experimental design
- 5 Data quality assessment
- 6 Conclusions
- Acknowledgments
- References
- Chapter Two: Processing macromolecular diffuse scattering data
- Abstract
- 1 Introduction
- 2 Theory
- 3 Data processing tutorial
- 4 Conclusions
- Acknowledgments
- References
- Chapter Three: Molecular-dynamics simulations of macromolecular diffraction, part I: Preparation of protein crystal simulations
- Abstract
- 1 Introduction
- 2 Methods and discussion
- 3 Summary
- Acknowledgments
- References
- Chapter Four: Molecular-dynamics simulations of macromolecular diffraction, part II: Analysis of protein crystal simulations
- Abstract
- 1 Introduction
- 2 Methods and discussion
- 3 Summary
- Acknowledgments
- References
- Chapter Five: MD simulations of macromolecular crystals: Implications for the analysis of Bragg and diffuse scattering
- Abstract
- 1 Introduction
- 2 Brief summary of X-ray scattering in crystals
- 3 MD simulations of biomolecular crystals
- 4 Bragg scattering and crystallographic refinement
- 5 Diffuse scattering
- 6 Conclusions
- Acknowledgments
- References
- Chapter Six: Modeling diffuse scattering with simple, physically interpretable models
- Abstract
- 1 Introduction
- 2 Set up and resources
- 3 Modeling disorder
- 4 Model evaluation
- 5 Discussion
- Acknowledgments
- References
- Chapter Seven: Interpreting macromolecular diffraction through simulation
- Abstract
- 1 Introduction
- 2 Present status and future prospects for diffuse scattering
- 3 Image simulation methods
- 4 Extending simulators to diffuse scattering
- 5 Complexity of the simulation
- 6 Parameter space and implications for simulation
- 7 Conclusions
- 8 Software availability
- Acknowledgements
- References
- Chapter Eight: Refinement of multiconformer ensemble models from multi-temperature X-ray diffraction data
- Abstract
- 1 Introduction
- 2 Collection of multi-temperature X-ray diffraction data
- 3 Single conformer model refinement
- 4 Multiconformer model refinement
- 5 Identifying temperature-dependent conformational changes
- 6 Summary and conclusions
- Acknowledgments
- References
- Chapter Nine: Combining temperature perturbations with X-ray crystallography to study dynamic macromolecules: A thorough discussion of experimental methods
- Abstract
- 1 Introduction
- 2 Sample cryocooling in macromolecular crystallography
- 3 Non-cryogenic macromolecular crystallography
- 4 Temperature as a tool for studying conformational heterogeneity and dynamics
- 5 Conclusion
- References
- Chapter Ten: Room temperature crystallography and X-ray spectroscopy of metalloenzymes
- Abstract
- 1 Introduction
- 2 Sample preparation
- 3 Sample delivery system
- 4 XES data collection
- 5 XRD data collection
- 6 Summary and conclusions
- Acknowledgments
- References
- Chapter Eleven: Exploring the structural dynamics of proteins by pressure perturbation using macromolecular crystallography
- Abstract
- 1 Introduction
- 2 Diamond anvil cell
- 3 Sample managing
- 4 DAC-based experiment
- 5 Data processing
- 6 Structure determination and refinement
- 7 Data analysis
- 8 Perspectives
- Acknowledgments
- References
- Edition: 1
- Volume: 688
- Published: September 19, 2023
- Imprint: Academic Press
- No. of pages: 412
- Language: English
- Hardback ISBN: 9780443159268
- eBook ISBN: 9780443159275
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Nozomi Ando
Nozomi Ando, PhD, is an Associate Professor of Chemistry and Chemical Biology at the Cornell University. Prof. Ando was born in Denver, CO and grew up in the suburbs of Boston, MA. She received her BS from MIT where she was a physics major and music performance minor. She was drawn to the spaceship-like feel of the Cornell High Energy Synchrotron Source (CHESS) and went to Cornell University for her PhD in physics. As a graduate student in Sol Gruner’s lab, she made her own diamond cells for high-pressure X-ray scattering studies. She then went on to work as a postdoctoral fellow in Cathy Drennan’s lab at MIT, where she developed a fascination with metalloenzymes. In 2014, she was appointed Assistant Professor of Chemistry at Princeton University and started a research program that combines X-ray physics and structural enzymology. In 2018, the Ando lab moved to Cornell University when she joined the faculty in Chemistry & Chemical Biology.
Affiliations and expertise
Associate Professor, Department of Chemistry and Chemical Biology, Cornell University, USARead Crystallography of Protein Dynamics on ScienceDirect