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Correlative Light and Electron MIcroscopy

  • 1st Edition, Volume 111 - August 10, 2012
  • Editors: Thomas Muller-Reichert, Paul Verkade
  • Language: English
  • Hardback ISBN:
    9 7 8 - 0 - 1 2 - 4 1 6 0 2 6 - 2
  • eBook ISBN:
    9 7 8 - 0 - 1 2 - 3 9 1 4 3 8 - 5

The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM) has been employed for decades to generate… Read more

Correlative Light and Electron MIcroscopy

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The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM) has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume of Methods in Cell Biology covers many areas of CLEM, including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in C. elegans embryos using CLEM, and capturing endocytic segregation events with HPF-CLEM.