Correlative Light and Electron Microscopy V
- 1st Edition, Volume 187 - April 30, 2024
- Imprint: Academic Press
- Editors: Thomas Müller-Reichert, Paul Verkade
- Language: English
- Hardback ISBN:9 7 8 - 0 - 3 2 3 - 9 5 1 4 1 - 8
- eBook ISBN:9 7 8 - 0 - 3 2 3 - 9 5 1 4 2 - 5
Correlative Light and Electron Microscopy V, Volume 187 in the Methods in Cell Biology series, highlights advances in the field, with this new volume presenting interesting c… Read more
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Request a sales quoteCorrelative Light and Electron Microscopy V, Volume 187 in the Methods in Cell Biology series, highlights advances in the field, with this new volume presenting interesting chapters on timely topics, including Orthotopic brain tumor models derived from glioblastoma stem-like cells, RNA sequencing in hematopoietic stem cells, Generation of inducible pluripotent stem cells from human dermal fibroblasts, In vitro preparation of dental pulp stem cell grafts combined with biocompatible scaffolds for tissue engineering, Gene expression knockdown in chronic myeloid leukemia stem cells, Identification and isolation of slow-cycling GSCs, Assessment of CD133, EpCAM, and much more.
- Provides the authority and expertise of leading contributors from an international board of authors
- Presents the latest release in the Methods in Cell Biology series
- Includes the latest information on the topic of development, characterization and applications in CAR T Cells
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- Cover image
- Title page
- Table of Contents
- Series Page
- Copyright
- Contributors
- Preface
- Chapter One How to apply the broad toolbox of correlative light and electron microscopy to address a specific biological question
- Abstract
- 1 Introduction
- 2 Instrumentation and materials
- 3 Methods
- 4 Discussion
- Acknowledgments
- References
- Chapter Two Some tips and tricks for a Correlative Light Electron Microscopy workflow using stable expression of fluorescent proteins
- Abstract
- 1 Introduction
- 2 Instrumentation and materials
- 3 Methods
- 4 Conclusion
- Acknowledgments
- References
- Chapter Three Targeting of membrane proteins with fluoronanogold probes for high-resolution correlative microscopy
- Abstract
- 1 Introduction
- 2 Instrumentations and materials
- 3 Methods
- 4 Discussion
- Acknowledgments
- References
- Chapter Four Correlative light and electron microscopy at defined cell cycle stages in a controlled environment
- Abstract
- 1 Introduction
- 2 Instrumentation and materials
- 3 Methods
- 4 Summary and future perspectives
- Acknowledgments
- References
- Chapter Five Severe acute respiratory syndrome coronavirus 2 (SARS-CoV2) culture and sample preparation for correlative light electron microscopy
- Abstract
- 1 Introduction
- 2 Instrumentation and materials
- 3 Methods
- 4 Concluding remarks
- References
- Chapter Six A correlated light and electron microscopy approach to study the subcellular localization of phosphorylated vimentin in human lung tissue
- Abstract
- 1 Introduction
- 2 Methods
- 3 Instrumentation and materials
- 4 Results and discussion
- 5 Conclusion
- Acknowledgments
- References
- Chapter Seven Array tomography of in vivo labeled synaptic receptors
- Abstract
- 1 Introduction and rationale
- 2 Instrumentation and materials
- 3 Methods
- 4 Results
- 5 Advantages
- 6 Limitations
- 7 Optimization and troubleshooting
- 8 Alternative methods/procedures
- Acknowledgments
- References
- Chapter Eight Correlative cryo-microscopy pipelines for in situ cellular studies
- Abstract
- 1 Introduction
- 2 Materials and instrumentation
- 3 Method
- 4 Troubleshooting and optimization
- 5 Discussion and conclusion
- 6 Summary
- 7 Safety considerations and standards
- Acknowledgments
- References
- Chapter Nine Building a super-resolution fluorescence cryomicroscope
- Abstract
- 1 Introduction
- 2 Overview
- 3 Construction
- 4 Results
- 5 Conclusions
- References
- Chapter Ten Imaging intracellular components in situ using super-resolution cryo-correlative light and electron microscopy
- Abstract
- 1 Introduction
- 2 Methods
- 3 Discussion
- References
- Chapter Eleven Toward quantitative super-resolution methods for cryo-CLEM
- Abstract
- 1 Introduction
- 2 Methods
- 3 Materials and equipment
- 4 Results
- 5 Discussion
- 6 Conclusions
- Acknowledgments
- References
- Chapter Twelve Laboratory based correlative cryo-soft X-ray tomography and cryo-fluorescence microscopy
- Abstract
- 1 Introduction and rationale
- 2 Discussion and outlook
- Acknowledgments
- References
- Edition: 1
- Volume: 187
- Published: April 30, 2024
- Imprint: Academic Press
- No. of pages: 336
- Language: English
- Hardback ISBN: 9780323951418
- eBook ISBN: 9780323951425
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Thomas Müller-Reichert
Thomas Müller-Reichert works in the Core Facility Cellular Imaging (CFCI), Faculty of Medicine Carl Gustav Carus, Technische Universität Dresden, Germany.
Affiliations and expertise
Core Facility Cellular Imaging (CFCI), Faculty of Medicine Carl Gustav Carus, Technische Universität Dresden, GermanyPV
Paul Verkade
Paul Verkade is a Professor of Bioimaging at the University of Bristol, UK where his research group works on the development and application of microscopy techniques to Biomedical questions. The main tools in the lab are Electron microscopy (EM) and Correlative Light Electron Microscopy (CLEM) in which fields he has published over 100 papers and edited 5 books on CLEM (including 4 Volumes of the Methods in Cell Biology series). PV obtained his PhD at the University of Utrecht, The Netherlands in 1996. Subsequently he did a post-doc at the EMBL, Heidelberg, Germany, after which he set up the electron microscopy unit at the newly formed Max Planck Institute for Molecular Cell Biology in Dresden, Germany from 2001. He moved to the UK in 2006 to set up another EM unit as part of an integrated LM and EM bioimaging facility, which facilitates CLEM workflows. He is actively involved in shaping the future microscopy landscape with roles in the Royal Microscopical Society and BioimagingUK and a current focus on putting volumeEM on the imaging map through community building and the organisation and co-chairing of the 1st Gordon Research Conference on vEM.
Affiliations and expertise
The University of Bristol, Bristol, UKRead Correlative Light and Electron Microscopy V on ScienceDirect