Correlative Light and Electron Microscopy IV
- 1st Edition, Volume 162 - March 8, 2021
- Latest edition
- Editors: Thomas Muller-Reichert, Paul Verkade
- Language: English
Correlative Light and Electron Microscopy IV, Volume 162, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters auth… Read more
Correlative Light and Electron Microscopy IV, Volume 162, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Besides the detailed description of protocols for CLEM technologies including time-resolution, Super resolution LM and Volume EM, new chapters cover Workflow (dis)-advantages/spiderweb, Serial section LM + EM, Platinum clusters as CLEM probes, Correlative Light Electron Microscopy with a transition metal complex as a single probe, SEM-TEM-SIMS, HPF-CLEM, A new workflow for high-throughput screening of mitotic mammalian cells for electron microscopy using classic histological dyes, and more.
- Contains contributions from experts in the field
- Covers topics using nano-SIMS and EDX for CLEM
- Presents recent advances and currently applied correlative approaches
- Gives detailed protocols, allowing for the application of workflows in one’s own laboratory setting
- Covers CLEM approaches in the context of specific applications
- Aims to stimulate the use of new combinations of imaging modalities
PhD students, Post-doctoral researchers, and imaging facility staff working in the life science research area with an interest in microscopy technologies and applying or aiming to apply Correlative Microscopy techniques to their research
1. Workflow (dis)-advantages / spiderwebOri Avinoam2. Serial section LM + EMErin M. Tranfield3. Platinum clusters as CLEM probesPaul Verkade4. Correlative Light Electron Microscopy with a transition metal complex as a single probePaul Verkade5. Detection EDX + EELSBen N. G. Giepmans6. SEM-TEM-SIMSLouise Jensen7. HPF-CLEMXavier Heiligenstein8. A new workflow for high-throughput screening of mitotic mammalian cells for electron microscopy using classic histological dyesThomas Müller-Reichert9. Three-dimensional on-section correlative light and electron microscopy of large cellular volumes using STEM tomographyKorbinian Buerger10. Accelerated procedure for approaching and imaging of optically branded ROI in tissueNatalia V. Gounko11. Volume-CLEMKedar Narayan12. Cryo SOFI CLEMRainer Kaufman13. Cryo fluo after Cryo lamellaPetr Chlanda14. Super resolution CLEMSílvia Pujals15. FIB-SEM + softwareAllon Weiner16. Correlia, CLEM softwareMatthias Schmidt17. Correlated Multimodality Imaging beyond CLEM: From in-vivo Preclinical Imaging to ex-vivo MicroscopyAndreas Walter18. Community effortsPaul Verkade
- Edition: 1
- Latest edition
- Volume: 162
- Published: March 8, 2021
- Language: English
TM
Thomas Muller-Reichert
Thomas Müller-Reichert is a Professor of Structural Cell Biology at the Technische Universität Dresden (TU Dresden, Germany). He is interested in how the microtubule cytoskeleton is modulated within cells to fulfill functions in mitosis, meiosis and abscission. The Müller-Reichert lab is mainly applying correlative light microscopy and electron tomography to study the 3D organization of microtubules in early embryos and meiocytes of the nematode Caenorhabditis elegans, and also in mammalian cells in culture. He has published over 75 papers and edited several volumes of the Methods in Cell Biology series on electron microscopy and CLEM.
TMR obtained his PhD at the Swiss Federal Institute of Technology (ETH) in Zurich and moved afterwards for a post-doc to the EMBL in Heidelberg (Germany). He was a visiting scientist with Dr. Kent McDonald (UC Berkeley, USA). Together with Paul Verkade, he set up the electron microscope facility at the newly founded Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG). Since 2010 he is a scientific group leader and head of the Core Facility Cellular Imaging (CFCI) of the Faculty of Medicine Carl Gustav Carus of the TU Dresden. He acted as president of the German Society for Electron Microscopy (Deutsche Gesellschaft für Elektronenmikroskopie, DGE) from 2018 to 2019.
He taught numerous courses and workshops on high-pressure freezing and Correlative Light and Electron Microscopy.
Affiliations and expertise
Professor of Structural Cell Biology, Technische Universitat Dresden, TU Dresden, GermanyPV
Paul Verkade
Paul Verkade is a Professor of Bioimaging at the University of Bristol, UK where his research group works on the development and application of microscopy techniques to Biomedical questions. The main tools in the lab are Electron microscopy (EM) and Correlative Light Electron Microscopy (CLEM) in which fields he has published over 100 papers and edited 5 books on CLEM (including 4 Volumes of the Methods in Cell Biology series). PV obtained his PhD at the University of Utrecht, The Netherlands in 1996. Subsequently he did a post-doc at the EMBL, Heidelberg, Germany, after which he set up the electron microscopy unit at the newly formed Max Planck Institute for Molecular Cell Biology in Dresden, Germany from 2001. He moved to the UK in 2006 to set up another EM unit as part of an integrated LM and EM bioimaging facility, which facilitates CLEM workflows. He is actively involved in shaping the future microscopy landscape with roles in the Royal Microscopical Society and BioimagingUK and a current focus on putting volumeEM on the imaging map through community building and the organisation and co-chairing of the 1st Gordon Research Conference on vEM.
Affiliations and expertise
The University of Bristol, Bristol, UKRead Correlative Light and Electron Microscopy IV on ScienceDirect