Concepts in Radiation Cell Biology
- 1st Edition - January 28, 1972
- Imprint: Academic Press
- Editor: Gary Whitson
- Language: English
- Paperback ISBN:9 7 8 - 0 - 1 2 - 4 3 3 4 0 4 - 5
- eBook ISBN:9 7 8 - 0 - 3 2 3 - 1 5 7 1 6 - 2
Concepts in Radiation Cell Biology summarizes current concepts related to the effects of radiation on cell biology, with emphasis on the underlying macromolecular basis for… Read more
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Request a sales quoteConcepts in Radiation Cell Biology summarizes current concepts related to the effects of radiation on cell biology, with emphasis on the underlying macromolecular basis for cellular changes in irradiated cells. It explores the effects of non-ionizing radiation, such as ultraviolet and visible light; the use of laser light in cellular studies; and the biological effects of ionizing radiation on cells. Results of ultraviolet studies implicating DNA as the main target macromolecule responsible for radiation injury, such as division delays, lethality, and delayed DNA replication, are presented. Divided into eight chapters, this volume begins with an overview of ultraviolet irradiation of DNA as well as the physical and biological properties of irradiated DNA. It then discusses methods used in the photoinactivation of viruses; the effects of ultraviolet radiation on bacteria; radiation-induced biochemical changes in protozoa; and techniques for the analysis of radiation-induced mitotic delay in synchronously dividing sea urchin eggs. The book also covers the effects of radiation on mammalian cells; the effects of ionizing radiation on higher plants; and the photodynamic effects of laser light on cells. This book is a valuable resource for cell biologists, as well as students and investigators who are seeking the necessary information for further experimentation in radiation cell biology.
List of Contributors
Preface
Chapter 1 Ultraviolet Irradiation of DNA
I. Introduction
II. Photophysics
III. Identification of Photoproducts
IV. DNA Photoproducts and Their Properties
V. Physical and Biological Properties of Irradiated DNA
VI. Miscellaneous Topics
VII. Summary
References
Chapter 2 Methods for Photoinactivation of Viruses
I. Introduction
II. Ultraviolet Light
III. Photodynamic Action
References
Chapter 3 The Effects of Ultraviolet Radiations on Bacteria
I. Ancient History
II. The Site of UV-Induced Inactivation: Changes in Polynucleotides
III. The Fate of Pyrimidine Dimers in DNA
IV. Measurements of Radiation Sensitivity
V. Effects of Pyrimidine Dimers in D N A on Macromolecular Synthesis
VI. The Physiology of Irradiated Cells
VII. Mutation Induction—Sublethal Damage
VIII. Perspectives
References
Chapter 4 Radiation-Induced Biochemical Changes in Protozoa
I. Introduction
II. Survey
III. Changes in DNA following UV-Irradiation
IV. Biochemical Effects of Ionizing Radiation
V. Extensions
VI. Summary
References
Chapter 5 Techniques for the Analysis of Radiation-Induced Mitotic Delay in Synchronously Dividing Sea Urchin Eggs
I. Introduction
II. General Procedures for Handling Gametes and Zygotes
III. Irradiation Techniques
IV. Quantitative Analysis of Mitotic Delay
V. Special Techniques
VI. Special Advantages and Disadvantages in Comparison with Partially Synchronized Growing Cells
VII. Conclusion
References
Chapter 6 The Effects of Radiation on Mammalian Cells
I. Introduction
II. The Normal Cell Cycle
III. Radiosensitivity of the Cell Cycle
IV. Chromosome Aberrations
V. Radiation Lethality and Cell Renewal Systems
VI. Radiosensitivity of the Immune Response
VII. Radiation and Cancer
References
Chapter 7 Ionizing Radiation Effects on Higher Plants
I. Introduction
II. Actions of Radiation on Chromosomes and Mitosis
III. Actions of Radiation on Cell Growth
IV. Actions of Radiation on Senescence and Death
V. Law of Bergonie and Tribondeau
VI. Separation of "Genetic" and "Physiological" Lethality
VII. Summary
References
Chapter 8 Photodynamic Action of Laser Light on Cells
I. Introduction
II. Resume of Some Characteristics and Guidelines Relating to Photodynamic Action
III. Examples of Photosensitizing Agents
IV. Matching the Absorption Spectrum of the Photosensitizing Agent with the Wavelength of the Light Source
V. Comparison of Some Properties of Lasers and Other Light Sources
VI. Examples of Selective Destruction of Tissues, Cells, or Cell Organelles by Photodynamic Action Using Laser Light
References
Author Index
Subject Index
- Edition: 1
- Published: January 28, 1972
- No. of pages (eBook): 296
- Imprint: Academic Press
- Language: English
- Paperback ISBN: 9780124334045
- eBook ISBN: 9780323157162
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