Colloidal Gold
Principles, Methods, and Applications
- 1st Edition - November 16, 2012
- Editor: M. A. Hayat
- Language: English
- Paperback ISBN:9 7 8 - 0 - 1 2 - 3 9 6 0 3 2 - 0
- eBook ISBN:9 7 8 - 0 - 3 2 3 - 1 3 9 0 2 - 1
Since its introduction in 1971, the development and application of colloidal gold as a marker in electron microscopy has been phenomenal. Colloidal gold has become the method of… Read more
Since its introduction in 1971, the development and application of colloidal gold as a marker in electron microscopy has been phenomenal. Colloidal gold has become the method of choice in immunocytochemistry and many areas of cell biology. This universal method is applicable to most microscopical systems including optical microscopy; scanning, transmission, and high voltage electron microscopy; photoelectron, photon, fluorescent darkfield, and epipolarization microscopy. Colloidal gold allows high and low resolution studies, enzyme and nucleic acid labeling, study of dynamic cellular processes, and virus detection. This book is among the first available to cover the principles and methodology of colloidal gold in microscopy.
- Methods are described step by step, to enable researchers to learn these complex procedures solely by reference to these books
- Problems and limitations of techniques are discussed
- Guides users to avoid problems and choose the correct procedures for specific applications
- Contributors are eminent authorities in their fields
Researchers, technicians, teachers, and students in cell biology, immunocytochemistry, histochemistry, and electron microscopy
P. Baudhuin, P. Van der Smissen, S. Beauvois, and P.J. Courtoy, Molecular Interactions between Colloidal Gold, Proteins, and Living Cells. A.D. Hyatt, Protein A*b1Gold: Nonspecific Binding and Cross-Contamination. G.R. Newman and J.A. Hobot, Role of Tissue Processing in Colloidal Gold Methods. G.R. Newman, LR White Embedding Medium for Colloidal Gold Methods. J.A. Hobot, Lowicryls and Low Temperature Embedding for Colloidal Gold Methods. M. Bendayan, The Enzyme*b1Gold Cytochemical Approach: A Review. D.A. Handley and C.M. Arbeeny, Preparation and Application of Lipoprotein*b1Gold Complex. S. Villaschi, Preparation and Application of Albumin-Gold Complex. F.W.K. Kan and P. Pinto da Silva, Label-Fracture Cytochemistry. D. Men,try and A.I. Basbaum, Colloidal Gold Conjugates for Retrograde Neuronal Tracing. J.A. Traas, Colloidal Gold Labeling of Microtubules in Cleaved Whole Mounts of Cells. J.E. Beesley, Colloidal Gold: Immunonegative Staining Method. S.M. Pietschmann, E.H.S. Hausmann, and H.R. Gelderblom, Immunogold Labeling of Viruses In Situ. P. Buma, Study of Exocytosis with Colloidal Gold and Other Methods. E.M. Herman, Colloidal Gold Labeling of Acrylic Resin-Embedded Plant Tissues. C. Ferrari, G. De Panfilis, and G.C. Manara, Preembedding Immunogold Staining of Cell Surface-Associated Antigens Performed on Suspended Cells and Tissue Sections. K. Takata, ColloidalGold*b1Lectin*b1Ruthenium Red Method for High Voltage Electron Microscopy. H. Mar and T.N. Wight, Correlative Light and Electron Microscopic Immunocytochemistry in Pre-embedded Resin Sections with Colloidal Gold. R.A. Wolber and T.F. Beals, Streptavidin-Gold Labeling for Ultrastructural In-Situ Nucleic Acid Hybridization. R. Rohringer, Detection of Proteins with Colloidal Gold. J.F. Hainfeld, Undecagold*b1Antibody Method. M.D. Waele, Silver-Enhanced Colloidal Gold for the Detection of Leukocyte Cell Surface Antigens in Darkfield and Epipolarization Microscopy. Each chapter includes references. Index.
- Edition: 1
- Published: November 16, 2012
- Language: English
MH
M. A. Hayat
Dr. Hayat has published extensively in the fields of microscopy, cytology, immunohistochemistry, immunocytochemistry, and antigen retrieval methods. He is Distinguished Professor, Department of Biological Sciences, Kean University, Union, New Jersey, USA.
Affiliations and expertise
Distinguished Professor, Department of Biological Sciences, Kean University, Union, NJ, USARead Colloidal Gold on ScienceDirect