Cinemicrography in Cell Biology

1st Edition - January 1, 1963
Editor: George G. Rose
Language: English
Paperback ISBN:
9 7 8 - 1 - 4 8 3 2 - 5 4 7 6 - 0
Hardback ISBN:
9 7 8 - 1 - 4 8 3 2 - 3 1 5 1 - 8
eBook ISBN:
9 7 8 - 1 - 4 8 3 2 - 7 2 8 0 - 1

Cinemicrography in Cell Biology focuses on factual information on the cine apparatus and techniques, special methods of analysis possible only with the use of cine equipment, and… Read more

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Cinemicrography in Cell Biology focuses on factual information on the cine apparatus and techniques, special methods of analysis possible only with the use of cine equipment, and data on tissues and cells derived from permanent records of film strips. The selection first offers information on modular design for time-lapse cinemicrography and general design of a comparative cinemicrographic equipment for tissue culture, including cine-film abstracting and general design and construction of cinemicrograph. The text then ponders on instrumentation for cinemicrography from a general purpose viewpoint and flying spot television microscopy. Discussions focus on ultraviolet microbeam irradiation combined with visible light phase-contrast television microscopy; ultraviolet flying spot microscope; and double-beam visible and ultraviolet flying spot television microscopy. The text elaborates on flying spot cytospectrophotometry of living cells; use of interference microscope for the study of cell movements and the quantitative analysis of changes in growing cells; and dynamics of the development of myogenic tissue under conditions of explantation and transplantation. The selection is highly recommended for readers interested in the use of cinemicrography in cell biology.

Contents

Contributors

Preface

Part I. Techniques

Modular Design For Time-Lapse Cinemicrography

I. Introduction

II. Components, General Description

III. Varieties Of Units

IV. Cine-Film Abstracting

V. Summary

References

General Design Of A Comparative Cinemicrographic Equipment For Tissue Culture

I. Introduction

II. General Design And Construction Of Cinemicrograph; Associated Equipment

III. Outline Of Certain Phases Of One Study Made With This Equipment

Iv. Discussion

References

Instrumentation For Cinemicrography From A General Purpose Viewpoint

I. Introduction

II. Light Source

III. Microscope

IV. Camera

V. Support

References

Flying Spot Television Microscopy

I. Introduction

II. The Ultraviolet Flying Spot Microscope

III. Double-Beam Visible And Ultraviolet Flying Spot Television Microscopy

Iv. Ultraviolet Microbeam Irradiation Combined With Visible Light Phase-Contrast Television Microscopy

References

Flying Spot Cytospectrophotometry Of Living Cells

I. Objectives And Problems

II. Analytical Studies Of Artifact Induction

III. The Vibrating Mirror Flying Spot Cytospectrophotometer

IV. Testing The System: Preliminary Results

V. Conclusion

References

The Use Of The Interference Microscope For The Study Of Cell Movements And The Quantitative Analysis Of Changes In Growing Cells

I. Introduction

II. Types Of Interference Microscopes

III. The Technique Of Time-Lapse Filming With The Interference Microscope

IV. Studies Of Cell Movements Using Interference Microscopy

V. Quantitative Measurements On Growing Cells

References

Analyses Of Variations In Intermitotic Time

I. Introduction

II. Variations In Intermitotic Time In Response To Changes In Extracellular Environment

III. The Internal Variability Of Cultures

IV. General Conclusion: The Role Of Cinemicrography

References

Part II. Tissues

Dynamics Of The Development Of Myogenic Tissue Under Conditions Of Explantation And Transplantation

I. Introduction

II. Neurotrophic Induction Of Differentiation Of The Myoblast

III. Metabolic Factor Of Growth And Differentiation Of Myogenic Tissue

IV. Experiments Of Controlling The Plastic Processes In Muscle Tissue

V. Discussion Of The Results

VI. Summary

References

Experiments On Induction And Effect Of Gamma Radiation In Mouse Lung Developing in Vitro

I. Introduction

II. Literature On Lung Organotypic Culture

III. Technique

IV. General Behavior Of The Whole Lung Rudiment

V. Branching Process

VI. Radiation Effect

VII. Epitheliomesenchymal Interaction

VIII. Reaggregation Of Epithelial And Mesenchymal Lung Rudiments

IX. Conclusions

References

Some Tissue Culture Studies Concerning Bone Resorption

I. Introduction

II. Bone Resorption And The Role Of The Osteoclast

III. The Regulation Of Bone Resorption By Oxygen Tension

IV. The Citric Acid Cycle And Bone Metabolism

V. Factors Modifying Bone Resorption In Tissue Culture

VI. Summary

References

Part III. Cells

Motion Picture Studies Of The Osteoclast

I. Introduction

II. Behavior Of Osteoclasts Isolated In Vitro

III. Pinocytosis In Osteoclasts

IV. Conclusions

References

Cleavage Of Mouse Egg

I. Introduction

II. Technique

III. Observations

References

Phagocytosis And Pinocytosis

I. Introduction

II. Dynamics Of Phagocytosis

III. Dynamics Of Pinocytosis

IV. Observations On The Uptake And Storage Of Large Molecular Substances

V. Fate Of Ingested Particulated And Colloidal Substances In The Cytoplasm

VI. Discussion And Conclusion

References

The Morphological And Structural Features Of Neurons In Vitro Studied By Phase-Contrast And Time-Lapse Movies

I. Introduction

II. General Features Of Explants Of Nerve Tissue

III. Living Neuron Structure

IV. Features And Significance Of The Terminal Expansions In Growing Neurites

V. The Manner Of Neurite Growth In Length

VI. Pinocytosis

VII. Interrelationships Between Neurites Growing In Vitro

References

Hormonal Influence On Goldfish Pigment Cells In Vitro

I. Introduction

II. Materials And Methods

III. Results

IV. Discussion

V. Summary And Conclusions

References

Cine Analysis Of Some Aspects Of Mitosis In Endosperm

I. Introduction

II. Materials And Methods

III. Observations

IV. Summary

References

Cytological Studies On Parasites And Host Cells Clay

I. Introduction And Scope Of Work

II. Intestinal Flagellates Of The Wood-Eating Cockroach

III. Intracellular Parasites

IV. Miscellaneous Parasites

V. Summary And Areas To Be Explored

References

Considerations On Nucleolar Physiology; The Importance Of Time-Lapse Cinemicrography

I. Introduction

II. Morphology

III. Physiology

IV. Discussion

References

Mosaic Patterns Of Stromal Cells In Tissue Cultures

I. Introduction

II. Materials And Methods

III. Results

IV. Discussion

References

Intercellular Interchange

I. Introduction

II. Methods

III. Observations

IV. Discussion

References

Author Index

Subject Index

Life Sciences

Physical Sciences & Engineering

Social Sciences & Humanities

Health

Cinemicrography in Cell Biology

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