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Elsevier

Cilia: From Mechanisms to Disease–Part B

  • 1st Edition, Volume 176 - April 21, 2023
  • Latest edition
  • Editors: Jose Manuel Bravo-San Pedro, Lorenzo Galluzzi
  • Language: English

Cilia: From Mechanisms to Disease Part B, Volume 176 of the Methods in Cell Biology series, presents interesting chapters on topics such as The challenge of dissecting gene… Read more

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Description

Cilia: From Mechanisms to Disease Part B, Volume 176 of the Methods in Cell Biology series, presents interesting chapters on topics such as The challenge of dissecting gene function in model organisms: tools to characterize genetic mutants and assess transcriptional adaptation in zebrafish, Human LUHMES and NES cells as models for studying primary cilia in neurons, Fixation methods and immunolabeling for cilia proteins in ciliary and extraciliary locations, Single-molecule imaging in the primary cilium, Methods to analyze primary cilia in mouse cardiac lesion model, Methods to study motile ciliated cell types in a zebrafish brain mode, and more.

Other sections cover Clinical and Molecular diagnosis in BBS (Bardet-Biedl syndrome), Modeling ciliopathies in patient-derived primary cells, Analysis of motility and mucociliary function of tracheal epithelial cilia, High-speed Super-resolution SPEED Microscopy to study Live Primary Cilium, Methods for siRNA delivery in retina explants, Methods to study primary cilia and autophagy in the brain, and more.

Key features

  • Offers a detailed overview of the protocols used to study cilia structure and various aspects of ciliary function
  • Provides an approach to the study of some diseases related to ciliary dysfunction, also known as ciliopathies
  • Written in an accessible style by renowned experts in the field

Readership

Students and entry-level scientists who are for the first time approaching the study of cilia, as well as experienced researchers.

Table of contents

1. The challenge of dissecting gene function in model organisms: tools to characterize genetic mutants and assess transcriptional adaptation in zebrafish
Magdalena Cardenas Rodriguez and Iain Drummond

2. Human LUHMES and NES cells as models for studying primary cilia in neurons
Peter Swoboda

3. Fixation methods and immunolabeling for cilia proteins in ciliary and extraciliary locations
Russell J. Ferland

4. Single-molecule imaging in the primary cilium
Lucien E. Weiss

5. Methods to analyze primary cilia in mouse cardiac lesion model
Alfredo Criollo Sr., Daniel Peña-Oyarzun, Rodrigo Troncoso, Joseph A. Hill, Sergio Lavandero and Mauricio Budini Sr.

6. Methods to study motile ciliated cell types in a zebrafish brain mode
Nathalie Jurisch-Yaksi

7. Clinical and Molecular diagnosis in BBS (Bardet-Biedl syndrome)
Diana Valverde

8. Modelling ciliopathies in patient-derived primary cells
Markus Schueler and Kai-Uwe Eckardt

9. Analysis of motility and mucociliary function of tracheal epithelial cilia
Koji Ikegami

10. High-speed Super-resolution SPEED Microscopy to study Live Primary Cilium
Weidong Yang

11. Method for siRNA delivery in retina explants
Florent Poulhès

12. Methods to study primary cilia and autophagy in the brain
Olatz Pampliega

13. Multi-color live-cell fluorescence imaging of primary ciliary membrane assembly dynamics
Christopher Westlake

Product details

  • Edition: 1
  • Latest edition
  • Volume: 176
  • Published: May 8, 2023
  • Language: English

About the editors

JB

Jose Manuel Bravo-San Pedro

Jose Manuel Bravo-San Pedro is currently a researcher at the Department of Physiology of the Complutense University of Madrid thanks to a Ramon y Cajal contract grant. He got his Ph.D. in biochemistry, cellular biology and genetics from the University of Extremadura (Caceres, Spain) in 2011, and he did a post-doctoral stage in the laboratory of Prof. Guido Kroemer. His main research interests have always been linked to autophagy, addressing this cellular process associated with neurodegenerative diseases or cancer and recently obesity and specifically related to problems in the correct functioning of the cilium. He is co-inventor of two patents and co-author of 110 publications indexed in PubMed in prestigious international journals, with h-index 45 and 23768 cites (Dec 2022).
Affiliations and expertise
Departamento de Fisiología, Facultad de Medicina, Universidad Complutense de Madrid, Madrid, Spain

LG

Lorenzo Galluzzi

Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome “La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology. Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals: OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience.
Affiliations and expertise
Assistant Professor of Cell Biology in Radiation Oncology, Department of Radiation Oncology, Weill Cornell Medical College, NY, USA

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