Chromatography
Fundamentals and applications of chromatography and related differential migration methods - Part A: Fundamentals and techniques
- 6th Edition, Volume 69A - April 16, 2004
- Imprint: Elsevier Science
- Editor: E. Heftmann
- Language: English
- Paperback ISBN:9 7 8 - 0 - 4 4 4 - 5 5 0 0 0 - 2
- eBook ISBN:9 7 8 - 0 - 0 8 - 0 4 7 2 2 4 - 9
Chromatography has emerged as the most important and versatile analytical method. The book is not only an updated version of Heftmann's classical text, but it covers areas of… Read more
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Request a sales quoteChromatography has emerged as the most important and versatile analytical method. The book is not only an updated version of Heftmann's classical text, but it covers areas of future importance, such as microfluidics and computer resources. Under his experienced guidance, authorities in each field have contributed their practical experience to an integrated treatment of modern micro analysis.
In Part A the theoretical basis of individual separation methods is explained and the technical aspects are illustrated. It includes the theory of gas and liquid chromatography as well as specific chromatographic techniques, such as size-exclusion, planar, ion, and affinity chromatography as well as various electrokinetic separation techniques. Microfluidics are covered for the first time and useful sources of analytical instruments are listed and evaluated.
In Part A the theoretical basis of individual separation methods is explained and the technical aspects are illustrated. It includes the theory of gas and liquid chromatography as well as specific chromatographic techniques, such as size-exclusion, planar, ion, and affinity chromatography as well as various electrokinetic separation techniques. Microfluidics are covered for the first time and useful sources of analytical instruments are listed and evaluated.
1. Each chapter written by an authority
2. Thorough treatment of the theoretical basis of separation methods
3. Practical guide for performing analyses
2. Thorough treatment of the theoretical basis of separation methods
3. Practical guide for performing analyses
Professional scientists that wish an in-depth understanding of the various separation science disciplines.
1. Theory of Chromatography (V.L. McGuffin).
Resolution. Principles of solute-zone dispersion.
Principles of solute-zone separation.
Optimization of chromatographic separations.
2. Column Liquid Chromatography (R.M. Smith).
Equipment for liquid-phase separations. Stationary-phase materials. Detection. Separation methods.
Liquid chromatography in sample preparation.
High-temperature separations.
Miniaturization.
Large-scale separations.
3. Affinity Chromatography (F.B. Anspach).
Chromatographic operations.
Affinity interactions at solid interfaces.
Affinity ligands.
4. Ion chromatography (C.A. Lucy, P. Hatsis).
Instrumentation.
Selectivity in ion chromatography.
Sample preparation.
Future directions.
5. Size-exclusion Chromatography (J. Silberring et al.).
Theory.
Columns.
Mobile phase.
Detectors.
Calibration.
Applications.
6. Planar Chromatography (Sz. Nyiredy).
Classification of planar chromatographic techniques.
Principles of planar chromatographic methods.
Principal factors in planar chromatography
Instrumentation.
Qualitative and quantitative analysis.
Preparative planar chromatography.
Special planar chromatographic techniques.
Comparison of various planar chromatographic techniques.
Trends in planar chromatography.
7. Electrokinetic chromatography (E. Kenndler, A. Rizzi).
Electro-osmotic flow in open and packed capillaries.
Electrochromatography with stationary phases.
Electrokinetic chromatography with pseudo-stationary phases.
Electrically driven vs. pressure-driven chromatography.
8. Gas Chromatography (P.J. Marriott).
Basic operating variables.
Enhanced and fast separations.
Sample introduction.
Detection.
9. Capillary Zone Electrophoresis (P.G. Righetti et al.).
The instrument.
The capillary.
How to modulate the electro-osmotic flow.
The buffers.
Modes of operation.
Micellar electrokinetic chromatography.
Biosensors.
10. Combined Techniques (W.M.A. Niessen).
Coupled columns.
Chromatography/spectrometry.
Liquid chromatography/mass spectrometry.
11. Microfabricated analytical devices (A. Guttman, J. Khandurina).
Capillary electrophoresis on microchips.
Applications.
System integration.
Modeling by computational fluid dynamics.
12. Instrumentation (R. Stevenson).
High-performance liquid chromatography.
Gas chromatography.
Thin-layer chromatography.
Supercritical-fluid chromatography.
Flash chromatography.
Electrophoresis.
Electrochromatography.
Future developments.
Resolution. Principles of solute-zone dispersion.
Principles of solute-zone separation.
Optimization of chromatographic separations.
2. Column Liquid Chromatography (R.M. Smith).
Equipment for liquid-phase separations. Stationary-phase materials. Detection. Separation methods.
Liquid chromatography in sample preparation.
High-temperature separations.
Miniaturization.
Large-scale separations.
3. Affinity Chromatography (F.B. Anspach).
Chromatographic operations.
Affinity interactions at solid interfaces.
Affinity ligands.
4. Ion chromatography (C.A. Lucy, P. Hatsis).
Instrumentation.
Selectivity in ion chromatography.
Sample preparation.
Future directions.
5. Size-exclusion Chromatography (J. Silberring et al.).
Theory.
Columns.
Mobile phase.
Detectors.
Calibration.
Applications.
6. Planar Chromatography (Sz. Nyiredy).
Classification of planar chromatographic techniques.
Principles of planar chromatographic methods.
Principal factors in planar chromatography
Instrumentation.
Qualitative and quantitative analysis.
Preparative planar chromatography.
Special planar chromatographic techniques.
Comparison of various planar chromatographic techniques.
Trends in planar chromatography.
7. Electrokinetic chromatography (E. Kenndler, A. Rizzi).
Electro-osmotic flow in open and packed capillaries.
Electrochromatography with stationary phases.
Electrokinetic chromatography with pseudo-stationary phases.
Electrically driven vs. pressure-driven chromatography.
8. Gas Chromatography (P.J. Marriott).
Basic operating variables.
Enhanced and fast separations.
Sample introduction.
Detection.
9. Capillary Zone Electrophoresis (P.G. Righetti et al.).
The instrument.
The capillary.
How to modulate the electro-osmotic flow.
The buffers.
Modes of operation.
Micellar electrokinetic chromatography.
Biosensors.
10. Combined Techniques (W.M.A. Niessen).
Coupled columns.
Chromatography/spectrometry.
Liquid chromatography/mass spectrometry.
11. Microfabricated analytical devices (A. Guttman, J. Khandurina).
Capillary electrophoresis on microchips.
Applications.
System integration.
Modeling by computational fluid dynamics.
12. Instrumentation (R. Stevenson).
High-performance liquid chromatography.
Gas chromatography.
Thin-layer chromatography.
Supercritical-fluid chromatography.
Flash chromatography.
Electrophoresis.
Electrochromatography.
Future developments.
- Edition: 6
- Volume: 69A
- Published: April 16, 2004
- Imprint: Elsevier Science
- No. of pages: 584
- Language: English
- Paperback ISBN: 9780444550002
- eBook ISBN: 9780080472249
EH
E. Heftmann
Affiliations and expertise
Orinda, CA, USARead Chromatography on ScienceDirect