Chemical and Synthetic Biology Approaches to Understand Cellular Functions - Part A

1. Targeted m⁶A reader proteins to study the epitranscriptome
Simone Rauch and Bryan C. Dickinson

2. Synthetic gene expression circuits regulating sexual reproduction
Nobuo Fukuda and Shinya Honda

3. Nucleotide resolution sequencing of N4-acetylcytidine in RNA
Justin M. Thomas, Keri M. Bryson and Jordan L. Meier

4. A guide for drug inducible genome editing with HIT systems
Chen Zhao, Shixian Wei and Yu Wang

5. A guide for drug inducible transcriptional activation with HIT systems
Chen Zhao, Shixian Wei and Yu Wang

6. Direct cloning and heterologous expression of natural product biosynthetic gene clusters by transformation-associated recombination
Jia Jia Zhang, Kazuya Yamanaka, Xiaoyu Tang and Bradley S. Moore

7. Salt-sensitive intein for large-scale polypeptide production
Yi-Zong Lee and Shih-Che Sue

8. Methods for the recombinant expression of active tyrosine kinase domains: Guidelines and pitfalls
M. Escarlet Díaz Galicia, Abdullah Aldehaiman, SeungBeom Hong, Stefan T. Arold and Raik Grünberg

9. Design, cloning and characterization of transcription factor-based inducible gene expression systems
Erik K.R. Hanko, Nigel P. Minton and Naglis Malys

10. Design, construction, and validation of optogenetic proteins
Colin P. O'Banion, Anwesha Goswami and David S. Lawrence

11. Overcoming component limitations in synthetic biology through transposon-mediated protein engineering
Joshua T. Atkinson, Bingyan Wu, Laura Segatori and Jonathan J. Silberg

12. Chemical biology of glycoproteins: From chemical synthesis to biological impact
Yaohao Li, Amy H. Tran, Samuel J. Danishefsky and Zhongping Tan

13. In bulla functional channel expression systems that mimic bacterial synthetic membranes
Masayuki Iwamoto and Shigetoshi Oiki

14. A mass spectrometry-based isotope-coded mass tag method to map thiol accessibility in biological systems
John E. Gadbery and Nicole S. Sampson

15. Quick-soaking of crystals reveals unprecedented insights into the catalytic mechanism of glycosyltransferases
David Albesa-Jové, Javier O. Cifuente, Beatriz Trastoy and Marcelo E. Guerin

16. Cysteine-ethylation of tissue-extracted membrane proteins as a tool to detect conformational states by solid-state NMR spectroscopy
Daniel K. Weber, Taysir Bader, Erik K. Larsen, Songlin Wang, Tata Gopinath, Mark Distefano and Gianluigi Veglia

17. Improved sensitivity and resolution of in-cell NMR spectra
David S. Burz, Leonard Breindel and Alexander Shekhtman

18. High-throughput methods in aptamer discovery and analysis
Kyle H. Cole and Andrej Lupták

19. Drop-in-well chamber for droplet interface bilayer with built-in electrodes
Kazuhiro Urakubo, Masayuki Iwamoto and Shigetoshi Oiki