Centrifugation in Density Gradients
- 1st Edition - January 28, 1982
- Imprint: Academic Press
- Author: C. A. Price
- Language: English
- Paperback ISBN:9 7 8 - 1 - 4 8 3 2 - 4 4 9 7 - 6
- eBook ISBN:9 7 8 - 1 - 4 8 3 2 - 8 2 0 6 - 0
Centrifugation in Density Gradients provides information pertinent to the fundamental aspects of density gradient centrifugation. This book discusses the benefits of density… Read more
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Request a sales quoteCentrifugation in Density Gradients provides information pertinent to the fundamental aspects of density gradient centrifugation. This book discusses the benefits of density gradient centrifugation to membrane-bound particles. Organized into nine chapters, this book begins with an overview of the method of differential or fractional centrifugation. This text then explores the physical basis of density gradient centrifugation. Other chapters deal with the nuts and bolts of density gradient centrifugation, the construction and composition of gradients, the properties and operation of centrifuge systems, and certain arcane but highly useful procedures. This book discusses as well density gradient centrifugation in the analytical ultracentrifuge. The final chapter deals with a collection of protocols for separating particles ranging in size from whole cells to macromolecules. This book is intended to be suitable for readers who need to separate biological particles. Biologists, chemists, biochemists, cytologists, physiologists, scientists, and research workers will also find this book useful.
ForewordPreface1 Particle Abstraction in Biology—An Introduction Text2 Origins of Density Gradient Centrifugation Text3 Sedimentation Theory: A Semiquantitative Approach 3.1 Behavior of Particles in Centrifugal Fields 3.2 Rate Separations 3.3 Equilibrium Density. Isopycnic Separations and Sedimentation Equilibrium References4 Sedimentation Theory: A More Rigorous Approach 4.1 Physical Description 4.2 Equation of Motion of a Single Particle in a Centrifugal Field 4.3 Motion of Dilute Collections of Particles in a Centrifugal Field 4.4 Motion of Boundaries and Zones References5 Gradient Materials and Construction of Gradients 5.1 Choice of Gradient Materials 5.2 Concentration Conventions and Stock Solutions 5.3 Gradient Generators 5.4 Density Measurements References6 Techniques of Preparative Density Gradient Centrifugation 6.1 Planning a Separation 6.2 Swinging-Bucket Separations 6.3 Zonal Separations Data Processing References7 Special Topics 7.1 Continuous-Flow Centrifugation with Isopycnic Banding 7.2 Flotation 7.3 Gradient Centrifugation in Angle and Vertical Rotors 7.4 Multiple-Alternate-Channel-Selection (MACS) 7.5 Reorienting Gradient Centrifugation 7.6 Seals: Polishing and Maintenance 7.7 Estimation of Sedimentation Coefficients 7.8 Transparent Rotors 7.9 Environmental Gradients 7.10 Sterilization 7.11 Other Uses of Density-Gradient Centrifugation References8 Analytical Centrifugation in Density Gradients 8.1 The Hardware of Analytical Centrifugation 8.2 Analytical Band Sedimentation in Density Gradients 8.3 Sedimentation-Diffusion Equilibrium in a Density Gradient References9 Protocols for the Separation and Analysis of Some Specific Particles 9.1 Whole Organisms 9.2 Whole Cells 9.3 Membrane-Bound Organelles 9.4 Multimolecular Systems 9.5 Macromolecules ReferencesAppendix A: Some Useful Units, Values, and Conversions A1. Mathematical Values and Relations A2. Physical and Chemical Values and Conversions A3. Values of ω2t for Different Speeds and Times A4. Relation of ω2t to RPM (Fig. A2) A5. Nomograph Relating Centrifugal Field, Radius, and Speed (Fig. A3)Appendix B: Properties of Particles B1. Natural Particles B2. Frictional Coefficients for Different Shapes B3. Artificial ParticlesAppendix C: Properties of Gradient Materials C1. Density and Viscosity of Aqueous Solutions of Sucrose C2. Density and Viscosity of Sucrose As Functions of Concentration C3. Refractive Index of Sucrose Solutions C4. Recipes for Preparing Stock Solutions of Aqueous Sucrose C5. Dilution of Stock Solutions of Sucrose C6. Coefficients for Density and Refractive Index of Alkali Salts C7. Additive Mixing Relations for Dilutions of CsCI Solutions C8. Density at 25°C of CsCI Solutions as a Function of Refractive Index C9. Properties of Sorbitol C10. Density, Refractive Index, and Viscosity of Ficoll C11. Properties of Colloidal Silicas C12. Density, Refractive Index, and Viscosity of Metrizamide C13. Radioopaque Agents C14. Density and Viscosity of Deuterium Oxide (Heavy Water) C15. Densities and Specific Viscosities of Mixtures of Cesium Chloride in H2O and D2O at 0.0°C C16. Values of β° for Several Gradient-Forming Salts C17. Integrals for Computing Sedimentation Times through Linear Sucrose GradientsAppendix D: Gradient Shapes D1. Parameters for Isokinetic Gradients of Sucrose for Two IEC Rotors D2. Parameters for Isokinetic Gradients in Sucrose for Four Spinco Rotors D3. Equivolumetric Gradients of Sucrose for Oak Ridge B-XIV Rotors D4. Equivolumetric Gradients of Sucrose for Oak Ridge B-XV Rotors D5. Construction of Hyperbolic Gradient for the Separation of Ribosomes D6. Acceleration Gradient D7. Shapes of Sucrose and NaCI Gradients at Sedimentation-Diffusion EquilibriumAppendix E: Zonal Rotors E1. ORNL Designation for Zonal RotorsAppendix F: Chemical Resistance of Various PlasticsAppendix G: Addresses of Some ManufacturersIndex
- Edition: 1
- Published: January 28, 1982
- Imprint: Academic Press
- No. of pages: 446
- Language: English
- Paperback ISBN: 9781483244976
- eBook ISBN: 9781483282060
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