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Cells and Tissues
2nd Edition - January 1, 1966
Editor: Arthur W. Pollister
eBook ISBN:9781483270777
9 7 8 - 1 - 4 8 3 2 - 7 0 7 7 - 7
Physical Techniques in Biological Research, Volume III, Part A: Cells and Tissues focuses on improvements and innovations in physical techniques involved in biological research on… Read more
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Physical Techniques in Biological Research, Volume III, Part A: Cells and Tissues focuses on improvements and innovations in physical techniques involved in biological research on cells and tissues. The selection first offers information on phase contrast and interference microscopy in cytology and birefringence and dichroism of cells and tissues. Topics include observational methods and results, quantitative techniques, nerve and muscle fibers, chloroplasts, cytoplasm, and nucleus. The book then examines X-ray microscopy and X-ray absorption analysis, including theoretical considerations of X-ray microscopy of biological materials and absorption and production of X-rays. The publication ponders on microtomy and manometric techniques for single cells and electron microscopy of microorganisms. Discussions focus on Cartesian diver respirometers, capillary respirometers, preliminary preparation of materials for drying, and techniques for studying dried specimens. The selection is highly recommended for students and readers interested in the physical techniques used in biological research.
Contents
List of Contributors v
Preface to First Edition of Volume III
Preface to Second Edition
Chapter 1 Phase Contrast and Interference Microscopy in Cytology
I. Introduction
II. Observational Methods and Results
III. Quantitative Techniques
References
Chapter 2 Birefringence and Dichroism of Cells and Tissue
I. Introduction
II. Plant Cell Walls
III. Cytoplasm
IV. Chloroplasts
V. Nerve Fibers
VI. Muscle Fibers
VII. Nucleus
References
Chapter 3 X-Ray Microscopy and X-Ray Absorption Analysis
I. Introduction
II. Production of X-Rays
III. Absorption of X-Rays
IV. The Formation of X-Ray Images
V. Theoretical Considerations of X-Ray Microscopy of Biological Materials
VI. Equipment
VII. Preparation of the Biological Material for Historadiography and a Discussion of Errors
VIII. Some Applications of Microradiography
References
Chapter 4 Microtomy
I. Introduction
II. The Specimen and Embedding
III. Microtomes
IV. Knives and Knife Sharpening
V. Measurement of Section Thickness as a Criterion of Over-all Performance
VI. Resolution and Section Thickness
References
Chapter 5 Manometric Techniques for Single Cells
I. Introduction
II. Capillary Respirometers
III. Cartesian Diver Respirometers
References
Chapter 6 Electron Microscopy of Microorganisms
I. Introduction
II. Preliminary Preparation of Materials for Drying