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Cell Structure and Function by Microspectrofluorometry
- 1st Edition - June 28, 2014
- Editor: Elli Kohen
- Language: English
- Paperback ISBN:9 7 8 - 1 - 4 8 3 2 - 4 1 7 9 - 1
- eBook ISBN:9 7 8 - 1 - 4 8 3 2 - 6 9 7 3 - 3
Cell Structure and Function by Microspectrofluorometry provides an overview of the state of knowledge in the study of cellular structure and function using microspectrofluorometry.… Read more
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Request a sales quoteCell Structure and Function by Microspectrofluorometry provides an overview of the state of knowledge in the study of cellular structure and function using microspectrofluorometry. The book is organized into six parts. Part I begins by tracing the origins of modern fluorescence microscopy and fluorescent probes. Part II discusses methods such as microspectroscopy and flow cytometry; the fluorescence spectroscopy of solutions; and the quantitative implementation of fluorescence resonance energy transfer (FRET) in the light microscope. Part III presents studies on metabolism, including the mechanism of action of xenobiotics; biochemical analysis of unpigmented single cells; and cell-to-cell communication in the endocrine and the exocrine pancreas. Part IV focuses on applications of fluorescent probes. Part V deals with cytometry and cell sorting. It includes studies on principles and characteristics of flow cytometry as a method for studying receptor-mediated endocytosis; and flow cytometric measurements of physiologic cell responses. Part VI on bioluminescence discusses approaches to measuring chemiluminescence or bioluminescence in a single cell and measuring light emitted by living cells.
Contributors
Preface
Tomas Hirschfeld—In Memoriam
Part I History
1. The Origins of Modern Fluorescence Microscopy and Fluorescent Probes
I. Introduction
II. The First Fluorescence Microscopes
III. Technical Progress
IV. Advances in Biomedical Applications
V. Modern Fluorescence Microscopy in Cell and Molecular Biology
VI. Development of Immunofluorescence
References
Part II Methods
2. Microspectroscopy and Flow Cytometry
3. From Solution Spectroscopy to Image Spectroscopy
I. Fluorescence Spectra
II. Fluorescence Excitation Spectrum
III. Fluorescence Lifetime and Yield
IV. Fluorescence Polarization
References
4. High-Resolution Fluorescence and Phase Microscopy in Conjunction with Micromanipulation for In Situ Study of Metabolism in Living Cells
I. Introduction
II. Resolution of Transmission and Fluorescence Microscopes
III. Microscope Methods
IV. Long-Working-Distance Condenser for Micromanipulation
V. Applications of Spectroscopy to Fluorescence Microscopy
VI. Instrument Design
VII. Application of Photography
VIII. Future Developments
References
5. FRET Microscopy: Digital Imaging of Fluorescence Resonance Energy Transfer. Application in Cell Biology
I. Introduction
II. Theory of Fluorescence Resonance Energy Transfer
III. Measurement of FRET: Data Acquisition and Analysis
IV. Experimental Methods and Results
V. Discussion and Future Prospects
References
6. Fluorescence Scanning Instrumentation
I. Introduction
II. Stage Scanning Microfluorometers
III. Laser Scanning Microfluorometers
IV. Composition of a Laser Scanning Microscope
V. Confocal Laser Scanning
VI. Characteristics of Laser Scanning
VII. Applications in Laser Scanning
References
7. Fluorescence Microscopy in Three Dimensions: Microtomoscopy
I. Introduction
II. Confocal Microscopy
III. Applications
IV. Discussion
References
8. Fluorescence Photochemical Techniques for the Study of Transport in Cytoplasm and Cytoplasmic Models
I. Introduction
II. Apparatus and Methodology
III. Applications to Cytoplasmic Transport
IV. Applications to Cytoplasmic Models In Vitro
V. Fluorescence Photoactivation
VI. Conclusions
References
9. Principles of Frequency-Domain Fluorescence Spectroscopy and Applications to Protein Fluorescence
I. Introduction
II. Comparison of Time and Frequency-Domain Measurements
III. Theory of Frequency-Domain Fluorometry
IV. Tryptophan Fluorescence from Proteins
V. 2-GHz Frequency-Domain Fluorometry
VI. Additional Applications of Frequency-Domain Fluorometry
VII. Future Developments
References
10. The First Picosecond in Vision
I. Introduction
II. Picosecond Time-Resolved Fluorescence Techniques
III. Picosecond Fluorescence Spectroscopy Results
IV. Discussion
References
Part III Metabolism
11. Microspectrofluorometry of Single Living Cells: Quo Vadis
I. Introduction
II. Instrumentation and Methods in Microspectrofluorometry
III. Biological Material
IV. Spatiotemporal Organization of Cell Metabolism
V. Spatiotemporal Mapping of Other Organelles: Lysosomes
VI. Fluorescence Detection of Multiorganelle Complexes Associated with the Cell's Detoxification Function
VII. Other Applications
VIII. Conclusions
References
12. Mechanism of Action of Xenobiotics: from Molecular Spectral Studies to Microspectrofluorometry of Living Cells
I. Introduction
II. Mechanism of Action of Polycyclic Aromatic Hydrocarbons
III. Mechanism of Action of Antipsoriatic Drugs
IV. Anticancer Drugs
V. Conclusions
References
13. Microfluorometry as a Tool for Biochemical Analysis in Unpigmented Single Cells
I. An Example of Convenient Apparatus
II. Resolution of a Complex Cell Fluorescence Spectrum
III. Evaluation of Enzymatic Activities in Intact Living Cells
IV. Use of Fluorescence with Pulsed Excitation
V. General Conclusions
References
14. Fluorescence in the Study of Direct Intercellular Communications: the Case of Pancreatic Cells
I. Introduction
II. Fluorescence Approaches to Direct Intercellular Communications
III. Intercellular Communication Network in the Pancreas
IV. Concluding Remarks
References
Part IV Fluorescent Probes
15. Approaches to the Study of Spatial and Temporal Changes in the Structure and Chemistry of Cells
I. Introduction
II. Approaches to the Study of Cellular Dynamics
III. Experimental Studies
IV. Prospectus
References
16. Fluorescence Studies of Microtubule Dynamics in Living Cells
I. Introduction
II. Microtubule Structure, Intrinsic Polarity, and Organization
III. Spindle Structure and Function
IV. Spindle Lability
V. Fluorescence Approaches to Analyzing Assembly Pathways
VI. Fluorescence Microscopy, Photobleaching, and Digital Image Processing
VII. Microtubule Assembly Occurs by a Dynamic Instability Mechanism
VIII. Comparison with Other Microtubule Arrays
IX. Future Directions
References
17. Optical Measurement of Membrane Potential in Invertebrate Ganglia and Mammalian Cortex
I. Introduction
II. Some Optical Signals are Potential-Dependent
III. Mechanisms
IV. Dyes
V. Recording Activity of Individual Neurons in a Molluscan Central Nervous System
VI. Monitoring Activity in Mammalian Brains
VII. Summary
References
18. Measurement of Free Calcium Concentration inside Single Cells with New Fluorescent Calcium Indicators
I. Introduction
II. Methods
III. Experimental Results
IV. Applications to Cell Systems
V. Future Directions
References
Part V Cytometry and Cell Sorting
19. Flow Cytometric Analysis of Ligand Binding and Endocytosis
I. Introduction
II. Ligand Binding
III. Ligand Internalization
IV. Ligand Acidification
V. Ligand Degradation
VI. Conclusion
References
20. Flow Cytometric Measurements of Physiologic Cell Responses
I. Introduction
II. Physiologic Probes
III. Instrumentation
References
21. Cellular Endogenous Fluorescence: a Basis for Preparing Subpopulations of Functionally Homogeneous Cells
I. Introduction
II. Technique of Autofluorescence-Activated Cell Sorting
III. Purification of Pancreatic B Cells
IV. Functional Heterogeneity in the Pancreatic B-Cell Population
V. Subpopulations Homogeneous in Cellular Hormone Content
VI. Subpopulations Homogeneous in Cellular Glucose Responsiveness
VII. Subpopulations Homogeneous in Sensitivity to Diabetogenic Agents
VIII. Conclusions
References
Part VI Bioluminescence
22. Approaches to the Measurement of Chemiluminescence or Bioluminescence in a Single Cell
I. Introduction
II. Materials and Methods
III. Results and Discussion
References
23. The Measurement of Light Emitted by Living Cells
I. Introduction
II. Electronically Excited States of Molecules
III. Methods for Absolute Calibration and Measurements in Bioluminescence
IV. Methods of Detection of Singlet Oxygen in Biological Reactions by Use of Chemiluminescent Probes
V. The Origin of Bioluminescence
VI. Bioluminescent Systems
VII. Colors of Firefly Bioluminescence
VIII. Experimental Evidence for the Optimization Model of Firefly Fluorescence
IX. Applications of Firefly Bioluminescence to Environmental Photobiology
X. Evolution of Bioluminescence in Bacteria
XI. Emission Spectrum of the Microsomal Chemiluminescence of a Proximate Carcinogen, 7,8-Diol-Benzo(a)Pyrene
XII. Conclusions
References
Index
- No. of pages: 490
- Language: English
- Edition: 1
- Published: June 28, 2014
- Imprint: Academic Press
- Paperback ISBN: 9781483241791
- eBook ISBN: 9781483269733
EK
Elli Kohen
Affiliations and expertise
University of Miami, Florida, U.S.A.Read Cell Structure and Function by Microspectrofluorometry on ScienceDirect