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Cell Separation
Methods and Selected Applications
1st Edition - June 5, 1984
Editors: Thomas G. Pretlow, Theresa P. Pretlow
eBook ISBN:9781483219400
9 7 8 - 1 - 4 8 3 2 - 1 9 4 0 - 0
Cell Separation: Methods and Selected Application, Volume 3 provides information pertinent to the design and application of methods for the separation of cells. This book covers a… Read more
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Cell Separation: Methods and Selected Application, Volume 3 provides information pertinent to the design and application of methods for the separation of cells. This book covers a variety of topics, including lymphoma cells, lectins, purification of cells from lung tumors, macrophage electrophoretic migration test, tissue heterogeneity, and characteristics of cultured cells. Organized into 13 chapters, this volume begins with an overview of the approaches in examining particular cell-surface properties and their role in the metastatic process. This text then examines lectins as important tools for identification and separation of cells, particularly of lymphocyte subpopulations. Other chapters consider the various methods that have been used to disperse rat pituitary tissue into single cells. This book discusses as well the different methods for isolating type II cells. The final chapter deals with the significance of having cell cultures homogeneous for a given cell type. This book is a valuable resource for cell biologists, experimental oncologists, hematologists, immunologists, and endocrinologists.
Contributors
Preface
Contents of Previous Volumes
1. Separation of Malignant Lymphoid Cells by Countercurrent Distribution
I. Introduction
II. Experimental Methods
III. Fractionation of RAW117 Lymphoma Cells
IV. Discussion of the Technique
References
2. Use of Lectins for Separation of Cells
I. Introduction
II. Methodology
III. Selected Applications
IV. Concluding Remarks
References
3. Enzymatic Disaggregation of Human Bronchogenic Carcinomas followed by Velocity Sedimentation of Cells
I. Introduction
II. Enzymatic Disaggregation of Tumors
III. Suspensions of Tumors: Storage and Composition
IV. Purification of Cells from Lung Tumors
V. Concluding Remarks
References
4. Macrophage Electrophoretic Migration (MEM) Test with Some Illustrative and Heuristic Applications
I. Historical Introduction
II. Basic Requirements
III. Preparation of Macrophages
IV. Preparation of Lymphocytes
V. Procedure: Cancer
VI. Applications
References
5. Implementation of Microtechniques to Resolve Tissue Heterogeneity
I. Introduction
II. Description of Microtechniques
III. Cell Differentiation in the Cellular Slime Mold Dictyostelium discoideum
IV. Adaptation of the Micromethods to Human Mammary Tumors
V. Conclusions
References
6. In Vitro Isolation of Malignant Cells from Small Cell Carcinomas