Carotenoids: Carotenoid and Apocarotenoid Biosynthesis, Metabolic Engineering and Synthetic Biology

1. Metabolomics-based analysis of carotenoids and related metabolites in various species via quantitative trait loci and genome wide association mapping approaches
Alisdair Fernie

2. Enzymatic isomerization of ζ-carotene mediated by the heme-containing isomerase Z-ISO
Jesus Beltran and Eleanore T. Wurtzel

3. Using bacteria for functional analysis of genes encoding carotenoid biosynthetic enzymes
Claudia Stange

4. Rice Callus as a High Throughput Platform for Synthetic Biology and Metabolic Engineering of Carotenoids
Changfu Zhu, Chao Bai, Maria Lourdes Gomez, Gerhard Sandmann, Teresa Capell, Can Baysal and Paul Christou

5. Transient expression in Nicotiana benthamiana: A simple platform to investigate genes encoding carotenoid biosynthesis enzymes from diverse algal lineages
Lauriebeth Leonelli

6. Protein-protein interaction techniques to investigate post-translational regulation of carotenogenesis
Li Li, Tianhu Sun, Xuesong Zhou, Jiping Liu and Sombir Rao

7. The isolation of sub-chromoplast structures from tomato and capsicum fruit
Paul David Fraser, Eugenia Enfissi, Laura Perez, Juliana Almeida, Margit Drapal, Harriet Berry and Marilsie Nogueira

8. Carrot protoplasts as a suitable method for protein subcellular localization
Claudia Stange

9. High throughput production and characterization of carotenoid enzymes for structural and functional studies
Brian Kloss

10. Production and structural characterization of the cytochrome P450 enzymes in carotene ring hydroxylation
Lin Liu, Guoqi Niu, Jia Wang and Qi Guo

11. Characterizing CYTOCHROME P450 Enzymes Involved in Plant Apocarotenoid Metabolism by Using Engineered Yeast System
Salim Al Babili, Yagiz Alagoz, Jianing Mi and Aparna Balakrishna

12. Preparation of carotenoid cleavage dioxygenases for crystallography
Philip Kiser, Xuewu Sui and Anahita Daruwalla

13. Heme-dependent enzymes involved in carotenoids biosynthesis in plants
Aimin Liu, Jiafeng Geng and Christopher Ian Davis

14. Tracking sub-plastidic localization of carotenoid metabolic enzymes with proteomics
Peter Knut Lundquist

15. Golden Rice and lessons learned for inspiring future metabolic engineering strategies and synthetic biology solutions
Ralf Welsch

16. Genetic transformation of Cassava to improve carotenoids: a protocol revisited
Paul Chavarriaga-Aguirre, Juan P. Arciniegas, Francisco J. Sánchez, Orlando Vacca, Arlen James Mosquera, Alejandro Brand and Adriana Medina

17. Elevating fruit carotenoid content in apple (Malus x domestica Borkh)
Charles Ampomah-Dwamena, Nitisha Bhargava, Caitlin Elborough, Kui Lin-Wang, Ria Rebstock, Sumathi Tomes and Cecilia Deng

18. Engineering microbial synthesis of carotenoids
Ian Wheeldon

19. The breeder's tool-box for enhancing the content of esterified carotenoids in wheat: from extraction and profiling of carotenoids to marker-assisted selection of candidate genes
Sergio G. Atienza, Dámaso Hornero-Méndez, María Dolores Requena-Ramírez and Cristina Rodríguez-Suárez

20. Drawing on enzyme promiscuity to create novel carotenoids via non-phytoene precursors
Daisuke Umeno

21. Plant abscisic acid receptor agonists and antagonists
Sean Cutler and Aditya Vaidya

22. A synthetic biology approach to study carotenoid production in Corynebacterium glutamicum: read-out by a genetically encoded biosensor combined with perturbing native gene expression by CRISPRi
Volker F Wendisch, Nadja A. Henke, Vanessa L. Göttl, Ina Schmitt and Petra Peters-Wendisch

23. Designing biosensors from a plant apocarotenoid receptor
Ian Wheeldon and Sean Cutler

24. Imaging Retinaldehyde-Protein Binding in Plants using a Merocyanine Reporter
Alexandra Jazz Dickinson, Martin Schnermann and Michael P. Luciano

25. Use of directed enzyme evolution to create novel biosynthetic pathways for production of non-natural and rare carotenoids
Maiko Furubayashi and Daisuke Umeno