Carcinogen-Driven Mouse Models of Oncogenesis

1st Edition, Volume 163 - March 26, 2021
Imprint: Academic Press
Editors: Lorenzo Galluzzi, Aitziber Buque
Language: English
Hardback ISBN:
9 7 8 - 0 - 1 2 - 8 2 2 5 3 4 - 9
eBook ISBN:
9 7 8 - 0 - 1 2 - 8 2 2 5 3 5 - 6

Carcinogen-Driven Mouse Models of Oncogenesis, Volume 163 contains a series of protocols written by world-leading experts in the field. Each manuscript provides a detailed… Read more

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Carcinogen-Driven Mouse Models of Oncogenesis, Volume 163 contains a series of protocols written by world-leading experts in the field. Each manuscript provides a detailed methodological description to drive carcinogen-mediated oncogenesis in mice. Chapters in this new release include Chemical carcinogenesis in mice as a model of human cancer: Pros and cons, MPA/DMBA-driven mammary carcinomas, Dimethylbenz(a)anthracene-Induced Mammary Tumorigenesis in mice, Urethane-induced lung carcinogenesis, Methylcholanthrene-induced fibrosarcomas, BBN-driven bladder carcinomas, Oral squamous cell carcinomas driven by 4NQO, Analyzing skin tumor development in mice by the DMBA/TPA model, and much more.

Other sections cover DSS/AOM-driven colorectal carcinomas, Diethylnitrosamine-induced liver tumorigenesis in mice, Two-stage 3-methylcholanthrene and butylated hydroxytoluene-induced lung carcinogenesis in mice, Lung carcinomas induced by NNK and LPS, Pristane-induced mammary carcinomas, The 4-NQO mouse model: an update on a well-established in vivo model of oral carcinogenesis, and more.

Cover image
Title page
Table of Contents
Copyright
Contributors
Preface: Chemical carcinogenesis in mice as a model of human cancer: Pros and cons
Acknowledgments
Disclosures
Chapter 1: MPA/DMBA-driven mammary carcinomas
Abstract
1: Introduction
2: Materials
3: Methods
4: Notes
5: Concluding remarks
Acknowledgments
Disclosures
Chapter 2: Dimethylbenz(a)anthracene-induced mammary tumorigenesis in mice
Abstract
1: Introduction
2: Material and methods
3: Discussion
4: Conclusion
Chapter 3: Urethane-induced lung carcinogenesis
Abstract
1: Introduction
2: Urethane preparation and mice treatment
3: Tumor lung evaluation
4: Conclusions
Acknowledgments
Chapter 4: Using methylcholanthrene-induced fibrosarcomas to study tumor immunology
Abstract
1: Introduction
2: Preparation of MCA suspension
3: Subcutaneous injection of MCA
4: Tumor growth and monitoring
5: Tumor isolation
6: In vitro growth
7: Tumorsphere assay
8: Preparation of samples for flow cytometry
9: In vivo blood vessel labeling
10: Optical clearing
11: Concluding remarks
Chapter 5: BBN-driven urinary bladder cancer mouse model
Abstract
1: Introduction
2: Mice treatment with BBN
3: Tissue preparation
4: Tissue fixation and paraffin embedding
5: Hematoxylin and eosin staining (standard procedure)
6: Immunohistochemistry staining for Ki67 staining
7: RNA, DNA and protein isolation from the frozen tissue
8: Protocol results
9: Concluding remarks
Acknowledgments
Funding
Chapter 6: 4NQO induced carcinogenesis: A mouse model for oral squamous cell carcinoma
Abstract
1: Introduction
2: 4NQO-induced OSCC mouse model
3: Common technique: Dissection of the tongue
4: Isolation of epithelium and tongue for protein and RNA analysis
5: Histological and visualization techniques for the tongue
6: Optical coherence tomography on the tongue
7: Histological analysis of tongues from control and 4NQO treated mice
8: Strength and limitation of the described approaches
9: Concluding remarks
10: Notes
Chapter 7: Analyzing skin tumor development in mice by the DMBA/TPA model
Abstract
1: Scientific background
2: Practical considerations
3: Comparison to human tumors
4: Conclusion
5: Procedure
Chapter 8: Induction of colitis-associated neoplasia in mice using azoxymethane and dextran sodium sulfate
Abstract
1: Introduction
2: Administration of AOM and DSS
3: Assessment of colonic inflammation and tumor burden during AOM/DSS administration
4: Tissue collection at the end of the treatment period
5: Disease assessment using harvested tissue
6: Concluding remarks
7: Notes
Acknowledgments
Chapter 9: Diethylnitrosamine-induced liver tumorigenesis in mice
Abstract
1: Introduction
2: DEN protocols
3: Mechanisms of DEN-mediated tumor initiation and progression
4: Genetic and phenotypic considerations
5: Tumor characterization
6: Tumor quantification
7: Important factors influencing DEN-mediated liver tumorigenesis
8: Safety and stability of DEN
9: Concluding remarks
10: Notes
Chapter 10: Two-stage 3-methylcholanthrene and butylated hydroxytoluene-induced lung carcinogenesis in mice
Abstract
1: Introduction
2: 2-Stage MCA/BHT model background
3: Protocol for the MCA/BHT model
4: Notes for optimization and troubleshooting
5: Conclusions
Acknowledgements
Chapter 11: Lung carcinomas induced by NNK and LPS
Abstract
1: Introduction
2: Materials
3: Treatments
4: Analysis
5: Concluding remarks
6: Notes
Acknowledgments
Chapter 12: Pristane-induced mammary carcinomas
Abstract
1: Introduction
2: Materials
3: Methods
4: Tumor growth assay
5: Response to treatment
6: Notes
7: Conclusion
Chapter 13: The 4-NQO mouse model: An update on a well-established in vivo model of oral carcinogenesis
Abstract
1: Introduction
2: Methods
3: Systematic review
4: Discussion
Funding
Disclosure of interest
5: Conclusions

Lorenzo Galluzzi

Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome “La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology. Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals: OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience.

Affiliations and expertise

Assistant Professor of Cell Biology in Radiation Oncology, Department of Radiation Oncology, Weill Cornell Medical College, NY, USA

Aitziber Buque

Aitziber Buqué (born 1980) is currently Post-Doctoral Fellow in the laboratory of Dr. Lorenzo Galluzzi with the Department of Radiation Oncology of the Weill Cornell Medical College (New York, NY, USA). Prior to joining the Galluzzi lab (2018), Aitziber Buqué was a Post-Doctoral Fellow in the Research Team “Apoptosis, Cancer and Immunity” at the Cordeliers Research Center (Paris, France; 2014-2018), under the direction of Dr. Guido Kroemer, after receiving her PhD from the University of the Basque Country (Leioa, Spain; 2013). Aitziber Buqué is interested in the molecular, cellular and immunological mechanisms whereby breast cancer evades immunosurveillance and resists to immunotherapy, and she is the author of more than 30 scientific articles in international peer-reviewed journals.

Affiliations and expertise

Post-Doctoral Fellow, Department of Radiation Oncology of the Weill Cornell Medical College, New York, NY, USA

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