
Capillary Gel Electrophoresis
- 1st Edition - December 4, 2021
- Imprint: Elsevier Science
- Authors: Andras Guttman, László Hajba
- Language: English
- Hardback ISBN:9 7 8 - 0 - 4 4 4 - 5 2 2 3 4 - 4
- eBook ISBN:9 7 8 - 0 - 0 8 - 0 9 3 1 3 5 - 7
Capillary Gel Electrophoresis and Related Microseparation Techniques covers all theoretical and practical aspects of capillary gel electrophoresis. It also provides an excellent… Read more
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Request a sales quoteCapillary Gel Electrophoresis and Related Microseparation Techniques covers all theoretical and practical aspects of capillary gel electrophoresis. It also provides an excellent overview of the key application areas of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods. It not only gives readers a better understanding of how to utilize this technology, but also provides insights into how to determine which method will provide the best technical solutions to particular problems. This book can also serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses.
- Covers all theoretical and practical aspects of capillary gel electrophoresis
- Excellent overview of the key applications of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods
- Teaches readers how to use the technology and select methods that are ideal for fundamental problems
- Can serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses
1. Introduction
1.1 Short history of capillary gel electrophoresis
1.2 The capillary gel electrophoresis system
1.2.1 Basic setup
1.2.2 The separation capillary
1.2.3 Power supply
1.2.4 Injection
1.2.5 Detection
1.2.6 Data acquisition
1.3 Separation modes in gel-filled capillary columns
1.3.1 CGE for molecular sieving
1.3.2 Capillary gel isoelectric focusing
1.3.3 Capillary gel isotachophoresis
1.3.4 Capillary affinity gel electrophoresis
1.4 Comparison with slab gel electrophoresis
References
2. Basic principles of capillary gel electrophoresis
2.1 The electrophoretic migration
2.2 Efficiency and resolution
2.3 Band broadening in capillary gel electrophoresis
2.4 Temperature effects and power dissipation
2.5 Complexation equilibrium
2.6 Electric field-mediated migration of biopolymers in sieving matrices
2.7 Micropreparative capillary gel electrophoresis
References
3. Separation matrix and column technology
3.1 Gels and polymer networks
3.1.1 Cross-linked (chemical) gels
3.1.2 Noncross-linked polymer solutions (physical gels)
3.2 Alternative matrices (composite gels, pluronics, agarose,
sol
3.2.1 Agarose-based gel compositions
3.2.2 Other alternative matrices
3.3 Capillary coatings
3.3.1 Covalent (chemical) coatings
3.3.2 Physical (noncovalent) surface coverage
3.4 Techniques for preparing gel-filled capillaries
References
4. Instrumentation
4.1 Sample introduction methods
4.1.1 Electrokinetic injection into high-viscosity gels
4.1.2 Hydrodynamic injection into low-viscosity polymer solutions
4.1.3 Sample preconcentration
4.1.4 Effect of sample overloading
4.1.5 Injection-related artifacts
4.2 Detection systems
4.2.1 UV absorbance and diode array detection
4.2.2 Laser-induced fluorescence detection
4.2.3 Indirect detection methods
4.2.4 Electrochemical and conductivity detection
4.2.5 Miscellaneous detector systems
4.2.6 Coupling to mass spectrometers
4.3 Operation variables
4.3.1 Gel concentration
4.3.2 Separation voltage and temperature
4.3.3 Capillary dimensions
4.3.4 Buffer systems
4.3.5 Nonaqueous electrophoresis, organic modifiers
References
5. Applications
5.1 Capillary gel electrophoresis of DNA
5.1.1 Analysis of single-stranded oligonucleotides
5.1.2 dsDNA fragments and PCR products
5.1.3 Large chromosomal DNA and pulsed-field electrophoresis
5.1.4 Antisense DNA and modified oligonucleotides
5.1.5 Biomedical and forensic applications
5.2 Capillary gel electrophoresis of proteins
5.2.1 Capillary SDS gel electrophoresis
5.2.2 Ultrafast separations
5.2.3 Capillary gel isoelectric focusing of proteins
5.2.4 Fluorescent labeling of proteins for SDS-CGE
5.2.5 Applications in biotechnology
5.3 Capillary gel electrophoresis of carbohydrates
5.3.1 Analytical glycobiology
5.3.2 Release of N- and O-linked oligosaccharides from glycoproteins
5.3.3 Fluorophore labeling of carbohydrates
5.3.4 Capillary gel electrophoresis separation of oligosaccharides
5.3.5 Selected other applications
5.4 Capillary affinity gel electrophoresis
5.4.1 Chiral affinity additives and chiral selectors
5.4.2 Capillary affinity gel electrophoresis of DNA
5.4.3 Other capillary affinity gel electrophoresis applications
References
6. Related microseparation techniques
6.1 Ultrathin-layer gel electrophoresis
6.1.1 DNA analysis
6.1.2 Protein analysis
6.2 Multidimensional approaches
References
7. Appendix
Manufacturers’ directory
Further reading
Abbreviations
Index
- Edition: 1
- Published: December 4, 2021
- No. of pages (Hardback): 390
- Imprint: Elsevier Science
- Language: English
- Hardback ISBN: 9780444522344
- eBook ISBN: 9780080931357
AG
Andras Guttman
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