Biotechnology of Filamentous Fungi
Technology and Products
- 1st Edition - October 22, 2013
- Imprint: Newnes
- Editor: David B. Finkelstein
- Language: English
- eBook ISBN:9 7 8 - 1 - 4 8 3 2 - 9 2 2 1 - 2
Biotechnology of Filamentous Fungi: Technology and Products provides a comprehensive discussion of the molecular biology, genetics, and biochemistry of filamentous fungi. It also… Read more
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Request a sales quoteBiotechnology of Filamentous Fungi: Technology and Products provides a comprehensive discussion of the molecular biology, genetics, and biochemistry of filamentous fungi. It also deals with general principles of biochemical engineering such as process design and scaleup. The book's main emphasis, however, is on the commercial significance of filamentous fungi. The book highlights the unique aspects of filamentous fungi along with those aspects common to most microorganisms studied in industries that use biotechnology. Filamentous fungi can generate a wide range of industrial products including primary metabolites such as organic acids, secondary metabolites such as β-lactam antibiotics, nonantibiotic drugs, and enzymes for use in food production. Whole organisms such as mushrooms can be used as well as organisms used as insecticides and herbicides. Filamentous fungi also qualify as potential hosts for the secretion of certain heterogeneous proteins such as mammalian proteins. However, not all things related to fungi are beneficial. Mycotoxins products by fungi can be lethal to humans; there is also a need to develop antifungal agents to destroy fungi that can kill animals and plants. These topics are important aspects of the biotechnology of filamentous fungi and are dealt with in this text.
Acknowledgments
1. Editorial Introduction
Part I. Principles of Technology
2. Isolation, Preservation, and Taxonomy
2.1 Taxonomy
2.2 Isolation
2.3 Preservation
References
Appendix: Literature References
3. Strain Improvement and Strain Stability
3.1 Requirements for Industrial Strain Improvement
3.2 Genetic Instability in Industrial Microorganisms
3.3 Sources of Genetic Material
3.4 Re-Isolation and Purification
3.5 Generation of Novel Genotypes
3.6 Expression
3.7 Screening
3.8 Yield Improvement by Process and Medium Development
3.9 Preservation
3.10 Conclusions
References
4. Growth Kinetics and Fermentation Scaleup
4.1 Fungal Growth
4.2 Scaleup of Fermentation
4.3 Economics
References
5. Regulation of Secondary Metabolism
5.1 Delayed Formation of Idiolites
5.2 Effectors of Idiolite Biosynthesis
5.3 Other Factors
5.4 Cessation of Biosynthesis
5.5 Improvement of Idiolite Production
References
6 Transformation
6.1 Transformation Techniques and Properties of Transformants
6.2 Vector Integration
6.3 Autonomously Replicating Vectors
6.4 Selectable Markers for Use with Wild-Type Organisms
6.5 Selectable Markers for Use with Mutant Hosts
6.6 Gene Isolation
References
7. Bioconversions
7.1 The Scope of Fungal Bioconversions
7.2 Fungi as Chemical Reagents
7.3 The Products of Fungal Bioconversions
7.4 The Future of Fungal Bioconversions
8. Screening for Antifungal Drugs
8.1 Antifungal Screens
8.2 Targets
8.3 Fungal Biology—Source of New Targets
8.4 Concluding Remarks
References
Part II. Products
9. Molecular Biology and Biochemistry of the β-Lactam Antibiotics
9.1 Historical Perspectives
9.2 Pathway for Synthesis of β-Lactams
9.3 Enzymes of β-Lactam Biosynthesis
9.4 Molecular Biology of Penicillin and Cephalosporin Producing Fungi
9.5 Application of Molecular Biology to β-Lactam Production
9.6 Future Prospects
References
10. Therapeutic Metabolites
10.1 Lovastatin
10.2 Cyclosporins
10.3 Ergot Alkaloids
10.4 Asperlicin
10.5 Future Products
References
11. Organic Acids
11.1 Citric Acid
11.2 Itaconic Acid
11.3 Gluconic Acid
11.4 Fumaric Acid
11.5 Miscellaneous Organic Acids
References
12. Insecticides and Herbicides
12.1 Fungi as Biological Control Agents
12.2 Mycoinsecticides
12.3 Mycoherbicides
12.4 Fungal Toxins as Mycopesticides
12.5 Commercialization
12.6 Genetic Manipulation
References
13. Food Enzymes
13.1 Proteases
13.2 Amylases and Glucoamylases
13.3 Pectic Enzymes
13.4 Lactases
13.5 α-Galactosidases
13.6 Dextranases
13.7 Cellulases
13.8 Hemicellulases
13.9 Lipases
13.10 Oxidation-Reduction Enzymes
13.11 Other Enzymes
13.12 Application of Gene Technology
References
14. Structure, Function, and Genetics of Cellulases
14.1 Production and Characterization of Fungal Cellulases
14.2 New Substrates for Cellulases
14.3 Cloning of Fungal Cellulolytic Enzyme Genes
14.4 Structure Determination of Trichoderma Cellulases
14.5 Functions of Fungal Cellulases
14.6 Expression of Cloned Cellulase Genes in Heterologous Host Systems
14.7 Genetic Engineering of the Cellulase Production Profiles of Trichoderma
14.8 Conclusions
References
15. Edible Mushrooms
15.1 The Mushroom Industry
15.2 Natural Life Cycle Barriers to Breeding in A. bisporus
15.3 Biotechnology—Breaking Mushroom Breeding Barriers
15.4 Diversity Analysis, Strain Typing, and Genetic Linkage Mapping of A. bisporus
15.5 Cytoplasmic Inheritance and Breeding in A. bisporus
15.6 Transformation
15.7 Developmental Studies
15.8 Concluding Remarks
References
16. Mycotoxins
16.1 Definitions and Background
16.2 Biosynthesis
16.3 Mycotoxins and Molecular Biology
16.4 Agricultural Ecology
16.5 Mycotoxin Control
16.6 Conclusions
References
Index
- Edition: 1
- Published: October 22, 2013
- Imprint: Newnes
- Language: English
- eBook ISBN: 9781483292212
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