Biotechnology Engineering
A Practical Approach on Bioprocess Development from Lab to Industrial Scale
- 1st Edition - August 1, 2025
- Imprint: Elsevier
- Editors: Mohammad Hadi Dehghani, Vinayaka B. Shet, Sandesh Kanthakere, Nabisab Mujawar Mubarak, Rama Rao Karri
- Language: English
- Paperback ISBN:9 7 8 - 0 - 4 4 3 - 3 1 4 7 6 - 6
- eBook ISBN:9 7 8 - 0 - 4 4 3 - 3 1 4 7 7 - 3
Biotechnology Engineering: A Practical Approach on Bioprocess Development from Lab to Industrial Scale comprehensively provides consolidated content related to biotechnology engine… Read more
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Request a sales quoteBiotechnology Engineering: A Practical Approach on Bioprocess Development from Lab to Industrial Scale comprehensively provides consolidated content related to biotechnology engineering that supports R&D to manufacture commercial products on an industrial scale to meet the market's requirements. The book's structured chapters, practical approach, and real-world case studies effectively fulfil knowledge and information needs in the bioprocessing and biotechnology field. The chapters cover a broad diversity of topics ranging from the basics of bioprocessing, scalable solutions, bioprocess optimization, collaboration opportunities with experts, and crucial information on regulatory compliance. As such the book bridges the gap between theory and real-world applications in the bioprocessing field. Biotechnology Engineering: A Practical Approach on Bioprocess Development from Lab to Industrial Scale serves as a desktop reference for academics, scientist, engineers and professionals working on bioprocess development, discussing bioprocess development with calculations and case studies, and highlighting modern bioreactors and software tools adopted in the bioprocess industry.
- Helps to understand the practical approach involved in bioprocess developments
- Includes calculations and case studies essential for scaling up bioprocesses independently from Erlenmeyer flask studies to bioreactors
- Provides insight into auditing requirements of bioprocess industry
Researchers in academia and industry and graduate students in bioprocess scale-up, bioreactor designing and biotechnology engineering
PART A: Cell culture and Kinetic study
1: Culturing of microbial, animal and plant cells in lab scale
1.1 Introduction to Cell Culturing Techniques
1.2 Microbial Cell Culture: Techniques and Methods
1.3 Animal Cell Culture: Techniques and Methods
1.4 Plant Cell Culture: Techniques and Methods
1.5 Growth Kinetics and Cell Counting: Calculations for Assessment and Analysis
1.6 Conclusions and References
2: Microbial growth and cell kinetics
2.1 Introduction
2.2 General mass balance, elemental balance, and heat balance calculations with respect to fermenter/bioreactor
2.3 Microbial growth and production (linear growth equation, yield values), Enzymatic conversion
2.4 Kinetic Calculations: Growth, Substrate Utilization, and Product Formation
2.5 Application of Kinetic Models in Bioprocess Development
2.6 Case Studies and Examples of Material Balance and Kinetic Calculations
2.7 Conclusions and References
3: Design, optimization, and Inoculum development for culturing the cells
3.1 Basics of Media Design for Cell Culturing
3.2 Optimization Techniques for Media Formulation
3.3 Inoculum Development Strategies
3.4 Calculations and Considerations for Inoculum Scaling
3.5 Case Studies and Examples of Media Design and Inoculum Development
3.6 Conclusions and References
4: Lab scale studies on Submerged Liquid Fermentation (SLF) and Solid State Fermentation (SSF)
4.1 Introduction to Submerged Liquid Fermentation (SLF)
4.2 Lab Scale Studies and Experimental Setup for SLF
4.3 Key Parameters and Calculations in SLF
4.4 Introduction to Solid State Fermentation (SSF)
4.5 Lab Scale Studies and Experimental Setup for SSF
4.6 Comparative Analysis of SLF and SSF: Advantages and Limitations
4.7 Conclusions and References
PART B: Bioreactor handling and operation
5: Fundamental of Bioreactors and accessories
5.1 Introduction to Bioreactors: Types and Classification
5.2 Components and Functioning of Bioreactors
5.3 Measurement and Control Instruments in Bioreactors
5.4 Sensors and Probes for Monitoring Bioreactor Parameters
5.5 Auxiliary Equipment and Accessories in Bioreactor Systems
5.6 Conclusions and References
6: Design Aspects and Aseptic Maintenance of Bioreactors
6.1 Introduction
6.2 Bioreactor Design Considerations: Vessel Geometry and Configuration
6.3 Aseptic Design Principles for Bioreactors
6.4 Sterility Assurance and Maintenance in Bioreactor Systems
6.5 Cleaning and Sterilization of Bioreactor Components
6.6 Validation and Documentation of Aseptic Conditions in Bioreactors
6.7 Conclusions and References
7: Cleaning Techniques for Bioreactors and Regulatory Standards
7.1 Introduction
7.2 Importance of Cleaning in Bioreactor Operations
7.3 Cleaning Agents and Disinfectants for Bioreactors
7.4 Cleaning Validation Protocols and Procedures
7.5 Regulatory Standards for Bioreactor Cleaning
7.6 Case Studies and Best Practices for Effective Bioreactor Cleaning
7.7 Conclusions and References
8: Bioreactor sterilization techniques and equipment
8.1 Introduction
8.2 Principles and Importance of Bioreactor Sterilization
8.3 Sterilization Methods for Bioreactors: Heat, Chemical, and Radiation
8.4 Equipment and Tools for Bioreactor Sterilization
8.5 Sterility Testing and Monitoring in Bioreactors
8.6 Case Studies and Troubleshooting in Bioreactor Sterilization
8.7 Conclusions and References
9: Standard Operating Procedures and Auditing in Bioprocess Industries
9.1 Introduction
9.2 Importance of Standard Operating Procedures (SOPs)
9.3 Development and Implementation of SOPs in Bioprocess Industries
9.4 Auditing and Compliance with Good Manufacturing Practices (GMP)
9.5 Documentation and Record-Keeping in Bioprocess Operations
9.6 Continuous Improvement and Quality Management Systems in Bioprocessing
9.7 Conclusions and References
10: Operation and control of Submerged Liquid Fermenters
10.1 Introduction
10.2 Overview of Submerged Liquid Fermenters (SLFs)
10.3 Operational Parameters and Control Strategies in SLFs
10.4 Monitoring and Adjustment of Bioreactor Conditions
10.5 Strategies for Scaling Up SLF Processes
10.6 Case Studies and Troubleshooting in SLF Operation and Control
10.7 Conclusions and References
11: Operation and control of submerged solid fermenters
11.1 Introduction
11.2 Overview of Submerged Solid Fermenters (SSFs)
11.3 Operational Parameters and Control Strategies in SSFs
11.4 Monitoring and Adjustment of Bioreactor Conditions
11.5 Strategies for Scaling Up SSF Processes
11.6 Case Studies and Troubleshooting in SSF Operation and Control
11.7 Conclusions and References
12: Equipment for downstream Product Recovery and Purification
12.1 Introduction to Product Recovery and Purification Processes
12.2 Types of Equipment for Product Recovery
12.3 Equipment for Product Purification: Separation and Purification Techniques
12.4 Criteria for Equipment Selection in Recovery and Purification Processes
12.5 Case Studies and Examples of Equipment Selection for Product Recovery and Purification
12.6 Conclusions and References
13: Modern Reactors and Software Tools in Bioprocess Development
13.1 Introduction
13.2 Evolution of Bioreactors: From Conventional to Modern Reactors
13.3 Single-Use Reactors: Advantages and Applications
13.4 Variable Volume Reactors: Benefits and Design Considerations
13.5 Software Tools for Bioprocess Development and Optimization
13.6 Integration of Modern Reactors and Software Tools: Case Studies and Future Trends
13.7 Conclusions and References
PART C: Bioreactor Scale up aspects and case studies
14: Scale-Up Rules and Reactor Accessories
14.1 Introduction to Scale-Up in Bioprocess Engineering
14.2 Principles and Considerations for Scale-Up
14.3 Rules and Guidelines for Successful Scale-Up
14.4 Reactor Accessories and Upgrades for Large-Scale Operations
14.5 Calculation of Scale-Up Parameters and Scaling Factors
14.6 Conclusions and References
15: Scale-Up Strategies for Submerged Liquid Fermentation
15.1 Overview of Scale-Up in SLF Processes
15.2 Scale-Up Considerations and Challenges in SLF
15.3 Strategies for Lab-Scale to Large-Scale SLF
15.4 Calculation of Key Parameters in SLF Scale-Up
15.5 Case Studies and Examples of SLF Scale-Up with Detailed Calculations
15.6 Conclusions and References
16: Scale-Up Strategies for Solid State Fermentation
16.1 Overview of Scale-Up in SSF Processes
16.2 Scale-Up Considerations and Challenges in SSF
16.3 Strategies for Lab-Scale to Large-Scale SSF
16.4 Calculation of Key Parameters in SSF Scale-Up
16.5 Case Studies and Examples of SSF Scale-Up with Detailed Calculations
16.6 Conclusions and References
17: Scale-Up Constraints from Lab Scale to Industrial Scale
17.1 Limitations and Challenges in Scaling Up from Lab to Industrial Scale
17.2 Factors Affecting Scale-Up Constraints in Bioprocesses
17.3 Process Validation and Optimization for Industrial-Scale Operations
17.4 Strategies to Address Scale-Up Constraints and Mitigate Risks
17.5 Case Studies and Lessons Learned in Lab-to-Industrial Scale-Up
17.6 Conclusions and References
PART D: Bioproducts
18: Commercial Bioproducts from Bioreactors
18.1 Introduction to Commercial Bioproducts
18.2 Biopharmaceuticals: Vaccines, Antibodies, and Therapeutic Proteins
18.3 Industrial Enzymes: Applications and Market Impact
18.4 Biofuels and Biochemicals: Production and Market Trends
18.5 Specialty Chemicals and Consumer Goods: Bioplastics and Biopolymers
18.6 Case Studies and Success Stories of Commercial Bioproducts
18.7 Conclusions and References
19: Bioproducts from Fermentation and their Economic Impact
19.1 Overview of Bioproducts from Fermentation Processes
19.2 Bioproducts in the Global Economy: Market Size and Growth
19.3 Economic Impact of Bioproducts in Various Industries
19.4 Sustainability and Green Bioproducts: Environmental Benefits
19.5 Future Prospects and Emerging Trends in Bioproduct Development
19.6 Conclusions and References
20: Manufacturing Techniques for Bioproducts
20.1 Introduction to Manufacturing Processes for Bioproducts
20.2 Upstream Processing: Fermentation and Cell Culturing
20.3 Downstream Processing: Separation, Purification, and Formulation
20.4 Process Optimization and Scale-Up in Bioproduct Manufacturing
20.5 Emerging Technologies and Innovative Manufacturing Approaches
20.6 Quality Control and Regulatory Considerations in Bioproduct Manufacturing
20.7 Conclusions and References
1: Culturing of microbial, animal and plant cells in lab scale
1.1 Introduction to Cell Culturing Techniques
1.2 Microbial Cell Culture: Techniques and Methods
1.3 Animal Cell Culture: Techniques and Methods
1.4 Plant Cell Culture: Techniques and Methods
1.5 Growth Kinetics and Cell Counting: Calculations for Assessment and Analysis
1.6 Conclusions and References
2: Microbial growth and cell kinetics
2.1 Introduction
2.2 General mass balance, elemental balance, and heat balance calculations with respect to fermenter/bioreactor
2.3 Microbial growth and production (linear growth equation, yield values), Enzymatic conversion
2.4 Kinetic Calculations: Growth, Substrate Utilization, and Product Formation
2.5 Application of Kinetic Models in Bioprocess Development
2.6 Case Studies and Examples of Material Balance and Kinetic Calculations
2.7 Conclusions and References
3: Design, optimization, and Inoculum development for culturing the cells
3.1 Basics of Media Design for Cell Culturing
3.2 Optimization Techniques for Media Formulation
3.3 Inoculum Development Strategies
3.4 Calculations and Considerations for Inoculum Scaling
3.5 Case Studies and Examples of Media Design and Inoculum Development
3.6 Conclusions and References
4: Lab scale studies on Submerged Liquid Fermentation (SLF) and Solid State Fermentation (SSF)
4.1 Introduction to Submerged Liquid Fermentation (SLF)
4.2 Lab Scale Studies and Experimental Setup for SLF
4.3 Key Parameters and Calculations in SLF
4.4 Introduction to Solid State Fermentation (SSF)
4.5 Lab Scale Studies and Experimental Setup for SSF
4.6 Comparative Analysis of SLF and SSF: Advantages and Limitations
4.7 Conclusions and References
PART B: Bioreactor handling and operation
5: Fundamental of Bioreactors and accessories
5.1 Introduction to Bioreactors: Types and Classification
5.2 Components and Functioning of Bioreactors
5.3 Measurement and Control Instruments in Bioreactors
5.4 Sensors and Probes for Monitoring Bioreactor Parameters
5.5 Auxiliary Equipment and Accessories in Bioreactor Systems
5.6 Conclusions and References
6: Design Aspects and Aseptic Maintenance of Bioreactors
6.1 Introduction
6.2 Bioreactor Design Considerations: Vessel Geometry and Configuration
6.3 Aseptic Design Principles for Bioreactors
6.4 Sterility Assurance and Maintenance in Bioreactor Systems
6.5 Cleaning and Sterilization of Bioreactor Components
6.6 Validation and Documentation of Aseptic Conditions in Bioreactors
6.7 Conclusions and References
7: Cleaning Techniques for Bioreactors and Regulatory Standards
7.1 Introduction
7.2 Importance of Cleaning in Bioreactor Operations
7.3 Cleaning Agents and Disinfectants for Bioreactors
7.4 Cleaning Validation Protocols and Procedures
7.5 Regulatory Standards for Bioreactor Cleaning
7.6 Case Studies and Best Practices for Effective Bioreactor Cleaning
7.7 Conclusions and References
8: Bioreactor sterilization techniques and equipment
8.1 Introduction
8.2 Principles and Importance of Bioreactor Sterilization
8.3 Sterilization Methods for Bioreactors: Heat, Chemical, and Radiation
8.4 Equipment and Tools for Bioreactor Sterilization
8.5 Sterility Testing and Monitoring in Bioreactors
8.6 Case Studies and Troubleshooting in Bioreactor Sterilization
8.7 Conclusions and References
9: Standard Operating Procedures and Auditing in Bioprocess Industries
9.1 Introduction
9.2 Importance of Standard Operating Procedures (SOPs)
9.3 Development and Implementation of SOPs in Bioprocess Industries
9.4 Auditing and Compliance with Good Manufacturing Practices (GMP)
9.5 Documentation and Record-Keeping in Bioprocess Operations
9.6 Continuous Improvement and Quality Management Systems in Bioprocessing
9.7 Conclusions and References
10: Operation and control of Submerged Liquid Fermenters
10.1 Introduction
10.2 Overview of Submerged Liquid Fermenters (SLFs)
10.3 Operational Parameters and Control Strategies in SLFs
10.4 Monitoring and Adjustment of Bioreactor Conditions
10.5 Strategies for Scaling Up SLF Processes
10.6 Case Studies and Troubleshooting in SLF Operation and Control
10.7 Conclusions and References
11: Operation and control of submerged solid fermenters
11.1 Introduction
11.2 Overview of Submerged Solid Fermenters (SSFs)
11.3 Operational Parameters and Control Strategies in SSFs
11.4 Monitoring and Adjustment of Bioreactor Conditions
11.5 Strategies for Scaling Up SSF Processes
11.6 Case Studies and Troubleshooting in SSF Operation and Control
11.7 Conclusions and References
12: Equipment for downstream Product Recovery and Purification
12.1 Introduction to Product Recovery and Purification Processes
12.2 Types of Equipment for Product Recovery
12.3 Equipment for Product Purification: Separation and Purification Techniques
12.4 Criteria for Equipment Selection in Recovery and Purification Processes
12.5 Case Studies and Examples of Equipment Selection for Product Recovery and Purification
12.6 Conclusions and References
13: Modern Reactors and Software Tools in Bioprocess Development
13.1 Introduction
13.2 Evolution of Bioreactors: From Conventional to Modern Reactors
13.3 Single-Use Reactors: Advantages and Applications
13.4 Variable Volume Reactors: Benefits and Design Considerations
13.5 Software Tools for Bioprocess Development and Optimization
13.6 Integration of Modern Reactors and Software Tools: Case Studies and Future Trends
13.7 Conclusions and References
PART C: Bioreactor Scale up aspects and case studies
14: Scale-Up Rules and Reactor Accessories
14.1 Introduction to Scale-Up in Bioprocess Engineering
14.2 Principles and Considerations for Scale-Up
14.3 Rules and Guidelines for Successful Scale-Up
14.4 Reactor Accessories and Upgrades for Large-Scale Operations
14.5 Calculation of Scale-Up Parameters and Scaling Factors
14.6 Conclusions and References
15: Scale-Up Strategies for Submerged Liquid Fermentation
15.1 Overview of Scale-Up in SLF Processes
15.2 Scale-Up Considerations and Challenges in SLF
15.3 Strategies for Lab-Scale to Large-Scale SLF
15.4 Calculation of Key Parameters in SLF Scale-Up
15.5 Case Studies and Examples of SLF Scale-Up with Detailed Calculations
15.6 Conclusions and References
16: Scale-Up Strategies for Solid State Fermentation
16.1 Overview of Scale-Up in SSF Processes
16.2 Scale-Up Considerations and Challenges in SSF
16.3 Strategies for Lab-Scale to Large-Scale SSF
16.4 Calculation of Key Parameters in SSF Scale-Up
16.5 Case Studies and Examples of SSF Scale-Up with Detailed Calculations
16.6 Conclusions and References
17: Scale-Up Constraints from Lab Scale to Industrial Scale
17.1 Limitations and Challenges in Scaling Up from Lab to Industrial Scale
17.2 Factors Affecting Scale-Up Constraints in Bioprocesses
17.3 Process Validation and Optimization for Industrial-Scale Operations
17.4 Strategies to Address Scale-Up Constraints and Mitigate Risks
17.5 Case Studies and Lessons Learned in Lab-to-Industrial Scale-Up
17.6 Conclusions and References
PART D: Bioproducts
18: Commercial Bioproducts from Bioreactors
18.1 Introduction to Commercial Bioproducts
18.2 Biopharmaceuticals: Vaccines, Antibodies, and Therapeutic Proteins
18.3 Industrial Enzymes: Applications and Market Impact
18.4 Biofuels and Biochemicals: Production and Market Trends
18.5 Specialty Chemicals and Consumer Goods: Bioplastics and Biopolymers
18.6 Case Studies and Success Stories of Commercial Bioproducts
18.7 Conclusions and References
19: Bioproducts from Fermentation and their Economic Impact
19.1 Overview of Bioproducts from Fermentation Processes
19.2 Bioproducts in the Global Economy: Market Size and Growth
19.3 Economic Impact of Bioproducts in Various Industries
19.4 Sustainability and Green Bioproducts: Environmental Benefits
19.5 Future Prospects and Emerging Trends in Bioproduct Development
19.6 Conclusions and References
20: Manufacturing Techniques for Bioproducts
20.1 Introduction to Manufacturing Processes for Bioproducts
20.2 Upstream Processing: Fermentation and Cell Culturing
20.3 Downstream Processing: Separation, Purification, and Formulation
20.4 Process Optimization and Scale-Up in Bioproduct Manufacturing
20.5 Emerging Technologies and Innovative Manufacturing Approaches
20.6 Quality Control and Regulatory Considerations in Bioproduct Manufacturing
20.7 Conclusions and References
- Edition: 1
- Published: August 1, 2025
- Imprint: Elsevier
- No. of pages: 520
- Language: English
- Paperback ISBN: 9780443314766
- eBook ISBN: 9780443314773
MH
Mohammad Hadi Dehghani
Prof. Dr. Mohammad Hadi Dehghani is a Full Professor at the Tehran University of Medical Sciences (TUMS), School of Public Health, Department of Environmental Health Engineering, Tehran, Islamic Republic of Iran. His scientific research interest includes environmental science. He is an editorial board member, guest editor, and reviewer in many internal and international journals and is a member of several international science committees around the world. He has authored or edited 16 books and more than 230 full papers published in peer-reviewed journals.
Affiliations and expertise
Professor, Tehran University of Medical Sciences (TUMS), School of Public Health, Department of Environmental Health Engineering, Tehran, IranVS
Vinayaka B. Shet
Dr Vinayaka B Shet is an Assistant Professor in the Department of Biotechnology Engineering, NMAMIT, Nitte (Deemed to be university), Nitte, Karnataka, India He received a Ph.D in Biotechnology from Visvesvaraya Technological University, India He has over 15 years of experience in Academics, 10 years in Research and 1 year in Industry His research interest includes the biomass pretreatment, fermentation, oenology, optimization, synthesis of nanoparticles and its applications He received young researcher award He has published over 30 papers, 7 book chapters and holding two Indian patent He is reviewer of various journals.
Affiliations and expertise
Assistant Professor, Department of Biotechnology Engineering, NMAMIT, Nitte, Karnataka, IndiaSK
Sandesh Kanthakere
Dr Sandesh K is an Assistant Professor in the Department of Biotechnology Engineering, NMAMIT, Nitte (Deemed to be university), Nitte, Karnataka, India He received Ph.D from Visvesvaraya Technological University, India and masters in chemical plant design from National Institute of Technology, Surathkal, Karnataka, India He has more than 12 years of academic experience and 10 years of research experience He has 13 publications in reputed journals, 3 book chapters and holding one Indian patent His research area includes Bioprocess modeling and optimization, fermentation and kinetic study and ABE fermentation He is reviewer of journals Energy Engineering and management and biomass conversion and biorefinery.
Affiliations and expertise
Assistant Professor, Department of Biotechnology Engineering, NMAMIT, Nitte, Karnataka, IndiaNM
Nabisab Mujawar Mubarak
Dr Mubarak Mujawar is currently an Associate Professor in the Department of Chemical Engineering at Curtin University, Sarawak Campus, Malaysia. His main research areas are carbon nanotube/nanofiber synthesis using microwave heating, synthesis of magnetic biochar and activated carbon using microwave technology, synthesis of biofuel using microwave heating, advanced materials syntheses such as hydro char, and graphene using microwave technology, functionalization of carbon nanotube for sensor application, application of CNTs and CNFs for removal liquids and gases pollutant, protein purification using carbon nanomaterials, immobilization of enzyme on carbon nanotubes and advanced material and reaction engineering etc.
Affiliations and expertise
Associate Professor, Department of Chemical Engineering, Curtin University, Sarawak Campus, Miri, Sarawak, MalaysiaRK
Rama Rao Karri
Dr. Rama Rao Karri is a professor (senior assistant) in the Faculty of Engineering at Universiti Teknologi Brunei. He has over 18 years of experience in academia, industry, and research. He has published 200+ research articles in reputed journals, book chapters, and conference proceedings.
Affiliations and expertise
Professor, Faculty of Engineering, University of Technology of Brunei, India