Biotechnological Production of Bioactive Phytochemicals of Medicinal Value

A Comprehensive Treatise

1st Edition - July 11, 2024
Editors: Anabela Romano, P. B. Kavi Kishor, Penna Suprasanna, T. Pullaiah, A. Ranga Rao
Language: English
Paperback ISBN:
9 7 8 - 0 - 4 4 3 - 2 1 8 1 8 - 7
eBook ISBN:
9 7 8 - 0 - 4 4 3 - 2 1 8 1 9 - 4

Biotechnological Production of Bioactive Phytochemicals of Medicinal Value: A Comprehensive Treatise covers a broad variety of methods for secondary metabolites productio… Read more

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Biotechnological Production of Bioactive Phytochemicals of Medicinal Value: A Comprehensive Treatise covers a broad variety of methods for secondary metabolites production (both pharmaceuticals and cosmeceuticals), compiling state-of-the-art material about the current knowledge of in vitro production for a large number of bioactive phytochemicals. Plants are a source of bioactive compounds and specialty chemicals such as ginsenosides; paclitaxel, artemisinin, veregen and nutraceuticals. Biopharmaceuticals are important in human healthcare, and herbal actives are gaining importance all over the world. With natural resources dwindling, in vitro production of secondary compounds on a commercial scale is being more and more required.

Besides providing an alternative technology to bypass difficulties, the plant tissue culture (used in a broad sense to include cell, tissue, and organ culture) offers many advantages. In vitro technology also facilitates novel means of conserving the genetic diversity of the germplasm of medicinal plants through cryopreservation, production of novel compounds through biotransformation, somatic hybridization, and selective gene transfer through recombinant DNA technology for enhancing metabolite production.

Cover image
Title page
Table of Contents
Copyright
List of contributors
Preface
Chapter 1. Plants to pharmacy: recapitulation of natural compounds transmuting human health
Abstract
1.1 Introduction
1.2 Plants as a source for diverse pharmaceutically important compounds
1.3 Production of bioactive compounds on an industrial scale
1.4 Factors affecting the accumulation of secondary plant products in vitro
1.5 Nanoparticles and secondary plant product accumulation
1.6 Future focus
Acknowledgments
Author contribution
Conflict of interest
References
Chapter 2. Production of bioactive metabolites in in vitro cultures of saffron (Crocus sativus L.)
Abstract
2.1 Introduction
2.2 Phytochemistry of Crocus sativus
2.3 Chemical and genetic diversity in Crocus sativus
2.4 Production of saffron metabolites through biotechnology techniques
2.5 Biological activity of in vitro cultures of Crocus sativus
2.6 Conclusions and future perspectives
Acknowledgments
Conflict of interest
References
Chapter 3. In vitro production of industrial valued bioactive secondary metabolites from selected medicinal plants of Sri Lanka
Abstract
3.1 Introduction
3.2 The plant of immortality: Aloe vera
3.3 Munronia pinnata (Wall.) Theob
3.4 Curcuma longa L. (Turmeric)
3.5 Gyrinops walla Gaertn. for agarwood production
Acknowledgments
Author contributions
References
Chapter 4. In vitro production of secondary metabolites by Thymus plants
Abstract
4.1 Introduction
4.2 Secondary metabolites of Thymus plants
4.3 Micropropagation of Thymus plants
4.4 In vitro production of secondary metabolites by Thymus plants
4.5 Concluding remarks
Acknowledgments
References
Chapter 5. In vitro production of anthocyanins
Abstract
Abbreviations
5.1 Introduction
5.2 Natural anthocyanin production
5.3 Demand
5.4 In vitro anthocyanin production methods
5.5 Scaling up in vitro anthocyanin production
5.6 Conclusion and future perspectives
References
Chapter 6. Prospecting phytochemical and biological of seedling extract of Libidibia ferrea (Fabaceae) produced from in vitro propagation
Abstract
6.1 Introduction
6.2 Material and Methods
6.3 Results
6.4 Discussion
6.5 Conclusion
Acknowledgment
Conflict of interest
References
Chapter 7. Properties, biotechnological production, and applications of rhinacanthins
Abstract
7.1 Introduction
7.2 Sources of Rhinacanthins
7.3 Chemistry and biosynthesis of rhinacanthins
7.4 Biotechnological production of rhinacanthins
7.5 Pharmaceutical applications of rhinacanthins
7.6 Conclusion
References
Chapter 8. Nanoparticle-mediated elicitation of plant secondary metabolites, in vitro and in vivo
Abstract
Abbreviation
8.1 Introduction
8.2 Secondary metabolite elicitation using nanomaterials
8.3 Nanoparticle exposure, absorption, and transport mechanisms in plant cells and tissues
8.4 Conclusion
References
Chapter 9. Recent trends in in vitro production of alkaloids from Rauvolfia serpentina
Abstract
List of abbreviations
9.1 Introduction
9.2 Rauvolfia serpentina
9.3 Bioactive compounds from Rauvolfia serpentina
9.4 Biosynthesis of terpenoid indole alkaloids in Rauvolfia serpentina
9.5 Alkaloid production in in vitro cultures of Rauvolfia serpentina
9.6 Conclusions and future prospects
Acknowledgment
References
Chapter 10. Experimental strategies to enhance in vitro production of plant secondary metabolites
Abstract
10.1 Introduction
10.2 Production of secondary metabolites in organ cultures
10.3 Synthesis of secondary metabolites using callus cultures
10.4 Synthesis of secondary metabolites through cell suspension culture
10.5 Elicitation of secondary metabolites in hairy root cultures
10.6 Agrobacterium and Ri transferred DNA genes
10.7 Scaling up of hairy roots and bioreactors
10.8 Conclusion
References
Chapter 11. An overview of the in vitro synthesis of anthraquinones in plant cell and organ cultures and elicitation strategies
Abstract
11.1 Introduction
11.2 Biosynthesis of anthraquinones
11.3 Chemistry of anthraquinones
11.4 Classification of anthraquinones
11.5 Occurrence of anthraquinones
11.6 Anthraquinone synthesis in plants through in vitro cultures
11.7 Elicitation of anthraquinone synthesis
11.8 Conclusions
References
Chapter 12. Secondary metabolite production in transgenic cultures: an overview over the last decade
Abstract
12.1 Introduction
12.2 SM production in transgenic cultures and transgenic plants expressing rol gene(s)
12.3 SM production in hairy root cultures (HRCs)
12.4 SM production in transgenic cultures via. metabolic engineering (ME)
12.5 Challenges and opportunities associated with SM production in transgenic cultures
12.6 Conclusions
Acknowledgments
References
Chapter 13. In vitro production of phytoecdysteroids in plants
Abstract
13.1 Introduction to phytoecdysteroids
13.2 Phytoecdysteroids diversity in different plant species
13.3 Biosynthetic pathways of phytoecdysteroids
13.4 In vitro production of phytoecdysteroids
13.5 Enhancement of phytoecdysteroids using different strategies
13.6 Conclusions
References
Chapter 14. Bacosides neurotropic molecules production by tissue cultures of Bacopa monnieri (L.) Wettst.
Abstract
Abbreviations
14.1 Introduction
14.2 Biochemical spectrum of B. monnieri
14.3 Biosynthesis of bacosides
14.4 Bacosides: potent neurotropic molecules
14.5 In vitro production of bacosides
14.6 Conclusion and future prospects
Acknowledgments
References
Chapter 15. Application of different tissue culture techniques for in vitro production of gymnemic acid: factors, approaches, and challenges to achieve higher yield
Abstract
15.1 Introduction
15.2 Biosynthesis of gymnemic acid from acetyl CoA
15.3 Mechanism of action of gymnemic acid
15.4 Enhanced production of gymnemic acid through plant tissue culture techniques
15.5 Factors affecting the production of gymnemic acid
15.6 Conclusions
Acknowledgments
Author contributions
Funding
References
Chapter 16. Adoption of biotechnologies for Withania somnifera (L.) Dunal for sustainable utilization
Abstract
16.1 Introduction
16.2 New varieties of Withania developed by CSIR-CIMAP
16.3 Importance of W. somnifera in traditional medicine
16.4 Importance of W. somnifera in modern medicine
16.5 Phytochemical composition of Withania
16.6 Biosynthesis pathway of withanolide
16.7 Metabolic engineering approaches for the production of withanolide
16.8 In vitro production of withanolides
16.9 Conclusion and future prospects
Acknowledgments
References
Chapter 17. In vitro cultures of some Asclepiadaceae members: a source for the production of secondary metabolites
Abstract
Abbreviations
17.1 Introduction
17.2 Role of tissue culture in synthesis of secondary metabolites
17.3 In vitro studies of medicinally important members of Asclepiadaceae
17.4 Synthesis of secondary metabolites through in vitro cultures
17.5 Important secondary metabolites of the selected asclepiadaceae members
17.6 Conclusions
References
Chapter 18. In vitro studies and hairy root development with reference to secondary metabolite production in medicinally important Solanum erianthum D. Don
Abstract
18.1 Introduction
18.2 Phytochemical composition of S. erianthum
18.3 In vitro production of solasodine
18.4 Hairy root culture and solasodine production
18.5 Conclusion and future prospects
Acknowledgments
References
Chapter 19. A nonsteroidal antiinflammatory drug boswellic acid from Boswellia serrata Roxb.
Abstract
19.1 Introduction
19.2 Oleo-gum resin
19.3 Strategies for augmented production of active metabolites
19.4 Synthesis of boswellic acid isomers and their activity
19.5 Genes used to synthesize boswellic acids in suitable host
19.6 Prospects
Acknowledgment
References
Chapter 20. Accumulation of anticancer compounds in cultured cells and hairy roots
Abstract
20.1 Introduction
20.2 Chemical and natural compounds that have antitumor activity
20.3 Anticancer compounds from callus and suspension cultures
20.4 Conclusions and perspectives
Acknowledgments
Author contributions
Funding
Data availability
References
Chapter 21. Applications of radiations and mutagenesis for the enhancement of plant secondary metabolites
Abstract
21.1 Introduction
21.2 Radiations and chemical mutagens
21.3 Secondary metabolites
21.4 Enhancement of secondary metabolites using elicitation and mutagenesis
21.5 Conclusions and future prospective
References
Chapter 22. In vitro hepatoprotective lignan production from Phyllanthus species
Abstract
22.1 Introduction
22.2 Lignans
22.3 Lignan biosynthetic pathway
22.4 In vitro propagation
22.5 Callus cultures
22.6 Lignan production in in vitro grown cultures
22.7 Genetic manipulation in Phyllanthus
22.8 Conclusions and future prospects
Acknowledgments
References
Chapter 23. Cardiotonic glycosides production in Digitalis: application of in vitro culture and transgenic approaches
Abstract
23.1 Introduction
23.2 In vitro culture and cardenolide production
23.3 Polyploidization, gamma irradiation, and in vitro cardenolides production
23.4 Cardenolide biosynthesis pathway
23.5 Hairy root cultures
23.6 Transgenic plants and cardenolide synthesis
23.7 Conclusion and future prospects
References
Chapter 24. Production of bioactive compounds from cell and organ cultures of Centella asiatica
Abstract
24.1 Introduction
24.2 Biosynthesis of centellosides from Centella asiatica
24.3 In vitro production of centellosides and other important secondary metabolites
24.4 Genetic transformation studies in Centella asiatica
24.5 Bioreactor scale studies for the production of centellosides
24.6 Conclusions and prospects
Acknowledgments
References
Index

Anabela Romano

Professor Anabela Romano is the leader of the Plant Biotechnology Lab since 1995, the year she joined the faculty at the University of Algarve, is the Director of the Department of Biological Sciences and Bioengineering at the Faculty of Science and Technology of the University of Algarve and director of the MED/Ualg - Mediterranean Institute for Agriculture, Environment and Development, and was previously Vice-Rector of the University of Algarve. Her current research focuses on the use of biotechnological approaches toward the propagation, conservation, and sustainable use of plant genetic resources and on the biological and chemical characterization of natural compounds. She is actively engaged in training graduate students and postdoctoral fellows (supervised 122 students) and teaches graduate and undergraduate-level courses in Plant Biotechnology, Genetic Engineering and Biotechnology, Natural Product Biotechnology, Medicinal Plants, and Phytotherapy, among others. She is the principal investigator of several research projects and evaluator of national and international research projects and grants. She has published 227 original papers, book chapters, and books, and has been invited to present lectures at specialized national and international congresses and other scientific meetings. She is currently an associate editor of the South African Journal of Botany (Elsevier).

Affiliations and expertise

Professor, Department of Biotechnology, MED–Mediterranean Institute for Agriculture, Environment and Development, Universidade do Algarve, Portugal

P. B. Kavi Kishor

Professor P.B.Kavi Kishor joined the Department of Genetics in the year 1981 and initiated the work on Plant Biotechnology. Worked at the Biotechnology Center, Ohio State University, Columbus, Ohio, USA (1992-1994) under the Rockefeller Foundation program. Visiting Professor at the Emory University, Atlanta, Georgia, USA; Visiting Professor at the Linkoping University, Linkoping, Sweden; and a Visiting Scientist at the Leibniz Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany. Published 286 papers including book chapters, edited/written seven books. Supervised 51 Ph.D.s as guide/co-guide. He has one patent to his credit.

Affiliations and expertise

Vignan’s Foundation for Science, Technology and Research, India

Penna Suprasanna

Professor Suprasanna Penna has worked as Head of Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Mumbai, and currently is a professor at the Homi Bhabha National Institute, Mumbai. He made significant contributions to plant science covering plant biotechnology, mutation breeding, stress tolerance and novel biopolymer. His work on technology development for radiation depolymerized oligo-chitosan has been successfully used in enhancing plant productivity in crop plants and vegetables. He has also contributed to molecular understanding of plant abiotic stress tolerance in crop plants and salinity-adaptive mechanisms in halophytes. He is on the editorial board of several national and international journals and published over 300 publications and has edited several books on salinity tolerance, genome editing and plant-metal interactions.

Affiliations and expertise

Professor, Homi Bhabha National Institute, Mumbai & Former-Head of the Nuclear Agriculture and Biotechnology Division in the Bhabha Atomic Research Centre, India

T. Pullaiah

Professor T. Pullaiah is a former Professor at the Department of Botany at Sri Krishnadevaraya University in Andhra Pradesh, India, where he has taught for more than 35 years. He was President of Indian Botanical Society (2014), President of the Indian Association for Angiosperm Taxonomy (2013). He was awarded the Panchanan Maheshwari Gold Medal, the Prof. P.C.Trivedi Medal, the Dr. G. Panigrahi Memorial Lecture award of the Indian Botanical Society and Prof. Y.D. Tyagi Gold Medal of the Indian Association for Angiosperm Taxonomy. Under his guidance 54 students obtained their doctoral degrees. He has authored 52 books, edited 23 books and published over 330 research papers, including reviews and book chapters. He was also a member of Species Survival Commission of the International Union for Conservation of Nature (IUCN). Professor Pullaiah received his PhD from Andhra University, India, attended Moscow State University, Russia, and worked as Post-Doctoral Fellow

Affiliations and expertise

Department of Botany, Sri Krishnadevaraya University, Anantapur, India

A. Ranga Rao

Dr Ranga Rao has carried out Postdoctoral Research work in Arizona State University, Arizona (USA). Dr. Ranga Rao has been working as Associate Professor and Senior Scientist in Department of Biotechnology, Vignan’s Foundation Science, Technology and Research University (Deemed to be University), India. He has 18 years of research experience. Previously Dr. Rang Rao worked as Visiting Senior Research Fellow, Visiting Assistant Dr. Ranga Rao published more than 50 papers in peer reviewed international and national journals, edited several books and has presented over 60 conferences/symposia/seminar in international conferences. and Global Biomass Production. He was selected for Junior Scientist of the Year Award (2015) by National Environmental Science Academy, New Delhi, India; honored TWAS-Young Affiliate (2014) by Regional Office of East South-East Asia and the Pacific Chinese Academy of Sciences (CAS), China; received Young Scientist Award (2014) at the World Food Congress by International Union of Food Science and Technology (IUFoST), Canada; Carl Storm International Diversity Fellowship Award (2010) by Gordon Research Conferences, USA. He is an associate fellow of Andhra Pradesh Akademi of Sciences (2019) Government of Andhra Pradesh, India and also fellow of the Society of Applied Biotechnology (2013), India. He has received research grants and travel grant fellowship as both international and national awards, under Young Scientist schemes. He is also serving as editorial board member and reviewer for reputed international and national journals.

Affiliations and expertise

Department of Biotechnology, Vignan’s Foundation for Science, Technology and Research (Deemed to be University), Vadlamudi, Guntur, Andhra Pradesh, India

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Biotechnological Production of Bioactive Phytochemicals of Medicinal Value

A Comprehensive Treatise

Purchase options

Innovate. Sustain. Transform.

Institutional subscription on ScienceDirect

Anabela Romano

P. B. Kavi Kishor

Penna Suprasanna

T. Pullaiah

A. Ranga Rao